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1.
The nervous systems of the turbellarians Microstomum lineare and Polycelis nigra and of the cestodes Diphyllobothrium dendriticum and Schistocephalus solidus were studied by means of the peroxidase-antiperoxidase (PAP) immunocytochemical method, with the use of antisera to the neuropeptides FMRF-amide, vasotocin, leu-enkephalin, met-enkephalin, neurotensin, somatostatin, and VIP, and to the bioamine serotonin. Anti-FMRF-amide positive perikarya and fibers occurred in all species, while the occurrence of the vertebrate brain-gut peptides and serotonin varied between the species. Anti-somatostatin and anti-VIP gave a negative result. Anti-FMRF-amide and anti-vasotocin positive immunoreactivity was found in the brain and gut of M. lineare, and in the CNS and the peripheral nerve net of the cestodes. We suggest that the brain-gut peptides of free-living flatworms act on the subtegumental region in the cestodes, which lack a gut but absorb their nutrients directly through the tegument. 相似文献
2.
We have examined actin cDNA of the flatworm Diphyllobothrium dendriticum (Cestoda). Actin is a contractile protein that has been implicated in a variety of developmental and cellular processes. It is highly conserved and present in all eukaryotic cells. It is of particular interest to analyze evolutionary preserved genes in flatworms, because ancestral flatworms are regarded to play a central role in the evolution of the metazoans (Barnes et al., 1998). Screening a cDNA library of D. dendriticum (UniZap XR, Stratagene) with a human -actin probe resulted in several positive clones. One of the cDNA inserts, Didactl, consisting of 1392 bp was completely sequenced. The established nucleotide sequence revealed a 5 untranslated region of 33 bp, the entire open reading frame of 1128 bp and a 3 untranslated region of 231 bp which ends in a stretch of 21 A residues. The potential polyadenylation signal (AATAAA) is located 14 bp upstream of the poly (A) tail. The deduced amino acid sequence of Didactl is 376 amino acids long. It is a typical invertebrate actin (Fyrberg et al., 1981) resembling more the cytoplasmic than the muscular isoforms of vertebrate actins. Didactl is for example 96% homologous to human cytoplasmic -actin but only 92.6% identical with human smooth muscle -actin. The actin proteins are generally encoded by a multigene family which differs in size from species to species. Most organisms have four to eight genes coding for actin in their genome, but the number of actin genes can also be over 20 (Hamelin et al., 1988). Sequence comparisons of Didactl and the partly sequenced cDNA clones indicate that D. dendriticum has at least four different genes coding for actin in its genome. 相似文献
3.
McVicar A. H. 1977. The bothridial hooks of Acanthobothrium quadripartitum Williams, 1968 (Cestoda: Tetraphyllidea): their growth and use in taxonomy. International Journal for Parasitology7: 439–442. Bothridial hooks are absent from juvenile Acanthobothrium quadripartitum in Raja naevus but develop to full size before there is much growth of the bothridia and strobila. There is a linear relationship between the lengths of the different components of the hooks during their development and it is suggested that the ratios of these lengths to the total hook length may be useful parameters in dstinguishing between species of Acanthobothrium. Principal component analysis of published hook dimensions gave good separation of most species of Acanthobothrium. 相似文献
4.
Peter W. Pappas 《Experimental parasitology》1982,54(1):80-86
The membrane-bound and solubilized (using Triton ×-100 or sodium dodecyl sulfate (SDS)) alkaline phosphohydrolase (APase) activities of the isolated brush border membrane of Hymenolepis diminuta require a divalent cation for maximum activity. Highest rates of substrate (p-nitrophenyl phosphate) hydrolysis are obtained with low concentrations of Mg2+ (1 mM), although low concentrations of Mn2+, Ca2+, or Zn2+ will also partially satisfy this requirement; higher concentrations of Mg2+ and Mn2+, and other divalent cations (Cu2+, Fe2+, and Pb2+), inhibit the membrane-bound APase activity. Solubilization of the membrane-bound enzyme in either Triton or SDS results in an increase in specific activity and Km, but has little effect on thermal stability of the APase activity. Phosphate, pyrophosphate, adenosine 5′-triphosphate, adenosine 5′-monophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate, and fructose 1,6-diphosphate inhibit substrate hydrolysis, and the relative affinities of these inhibitors for the APase enzyme are altered only slightly upon solubilization. Graphic analyses of data from inhibitor studies indicate that all eight inhibitors will inhibit membrane-bound and solubilized APase activities 100% at high inhibitonsubstrate ratios. Molybdate, F?, 2-mercaptoethanol, cysteine, and p-chloromercuribenzoate inhibit membrane-bound APase activity. Inhibitor data indicate that if more than one enzyme is responsible for the APase activity of the brush border membrane of H. diminuta, the enzymes cannot be differentiated on the basis of substrate specificity. 相似文献
5.
The uptake of leucine through the tegument of Cotugnia digonopora, a cestode found in the fowl intestine, occurs by a process of active transport. The Kt of transport is 0.87 mM and the Vmax is 0.223 μmol/min/g. Uptake of the amino acid is competitively inhibited by valine (Kt = 1.30 mM). Potassium cyanide and 2,4-dinitrophenol do not completely block the entry of leucine into the parasite. 相似文献
6.
Studies of regulatory metabolism in Moniezia expansa: the role of phosphoenolpyruvate carboxykinase.
Phosphoenolpyruvate carboxykinase (PEPCK) from M. expansa has been partially purified and its behaviour in a range of different assay conditions has been determined. Different PEPCK's were found in the cytosol and mitochondria. Some kinetic parameters for each are presented. Both enzymes are activated by Mn2+; cytosolic PEPCK is also activated by Mg2+. The enzymes have pH optima in the range 6·4–7·0. They do not differ with respect to their apparent affinities for inosine and guanosine diphosphates, but the latter allows higher maximal activity. Little activity is observed with adenosine diphosphate. Adenosine and inosine triphosphates exert weak inhibitory effects on the Mn2+ activated enzymes; a much strongsr inhibition is exerted on the cytosolic enzyme when activated by Mg2+. A number of non-nucleotide compounds were tested for possible inhibitory effects with no success. The forward and back reactions catalyzed by PEPCK proceed at similar rates, suggesting that the enzyme may be readily raversible in vivo. 相似文献
7.
8.
N. M. Biserova 《Cell and Tissue Biology》2008,2(3):253-264
Until now, there has been no answer to the question of whether specialized glial cells exist in the nervous system of platyhelminths. The identification of these cells in parasitic flatworms is difficult due to their organization as parenchymal animals. The goal of this study was to reveal and describe structural elements corresponding to the term glia in the CNS of the parasitic flatworm Grillotia erinaceus (Cestoda: Trypanorhyncha). Three types of glial cells are revealed. The first type consists of fibroblast-like cells located in the cerebral ganglia that contain fibrils and excrete onto the surface fibrillar material and possess desmosomes; the presumable function of fibroblast-like glial cells is the isolation and support of ganglionar neurons. Glial cells of the second type form a myelin-like envelope of giant axons and bulbar nerves of the scolex and have laminar cytoplasm; they are numerous and exceed the number of neurons in the composition of nerves. Glial cells of the third type form multilayer envelopes in the main nerve cords and make contacts with the excretory epithelium; however, specialized junctions with neurons were not found. The existence of glia in other free living and parasitic flatworms is discussed. 相似文献
9.
A F Grammeltvedt 《International journal for parasitology》1973,3(3):321-327
Grammeltvedt Anne-François 1973. Differentiation of the tegument and associated structures in Diphyllobothrium dendriticum Nitsch 1824 (Cestoda : Pseudophyllidea). An electron microscopical study. International Journal for Parasitology3: 321–327. The differentiation of the tegument and associated structures of the coracidium, procercoid, plerocercoid and adult is described. The embryophore is composed of four zones and is covered by a fibrous layer resembling a glycocalyx. The oncospheral plasma membrane is extensively folded. A typical cestode tegument, with a distal and perinuclear cytoplasm, is probably already existing in the coracidium. The formation of the microvilli starts after about three days in the copepod host. In young procercoids ribosomes and Golgi complexes were observed in the distal cytoplasm. These organelles disappear at later stages. The infective procercoid has a typical tegument. The microvilli are shaped like a thorn compressed from the sides. They have an electron dense tip and a less dense base in which microfilaments are seen. Bodies, called disc-shaped and lamellated bodies, are described. The microvilli of the plerocercoid are characterized by a great variation in shape. The villi are bounded by two unit membranes. The lamellated bodies are especially well developed. The adult microvilli are uniform in shape. The lamellated bodies are few in young adults and disappear in mature worms. 相似文献