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壳聚糖对黄瓜幼苗抗盐的协同生理作用研究   总被引:24,自引:2,他引:22  
在200 mm o l.L-1N aC l胁迫条件下,采用根部注射结合叶面喷施的方法,研究了不同浓度(0、50、100、150、200、250 m g.L-1)壳聚糖(Ch itosan,CTS)对黄瓜(Cucum is sa tivus L.)幼苗抗盐的生理作用。结果表明:CTS能够显著促进黄瓜幼苗的生长,降低植株盐害指数,最高幅度达36.2%(P<0.01);显著提高幼苗叶片游离脯氨酸(P ro)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等抗氧化酶活性(P<0.01);有效降低叶片细胞脂质过氧化产物丙二醛(M DA)含量和电解质渗透率(P<0.01);筛选出CTS最佳处理浓度为150 m g.L-1,持效期8 d以上。研究表明适宜浓度的CTS具有增强活性氧清除能力、保护生物膜功能、提高黄瓜幼苗抗盐的协同生理作用。  相似文献
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A histidine kinase-based signaling system has been proposed to function in ethylene signal transduction pathway of plants and one ethylene receptor has been found to possess His kinase activity. Here we demonstrate that a His kinase-like ethylene receptor homologue NTHK1 from tobacco has serine/threonine (Ser/Thr) kinase activity, but no His kinase activity. Evidence obtained by analyzing acid/base stability, phosphoamino acid and substrate specificity of the phosphorylated kinase domain, supports this conclusion. In addition, mutation of the presumptive phosphorylation site His (H378) to Gln did not affect the kinase activity whereas deletion of the ATP-binding domain eliminated it, indicating that the conserved His (H378) is not required for the kinase activity and this activity is intrinsic to the NTHK1-KD. Moreover, confocal analysis of NTHK1 expression in insect cells and plant cells suggested the plasma membrane localization of the NTHK1 protein. Thus, NTHK1 may represent a distinct Ser/Thr kinase-type ethylene receptor and function in an alternative mechanism for ethylene signal transduction.  相似文献
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Zhang ZG  Zhou HL  Chen T  Gong Y  Cao WH  Wang YJ  Zhang JS  Chen SY 《Plant physiology》2004,136(2):2971-2981
Ethylene plays important roles in plant growth, development, and stress responses. Two ethylene receptors, ETR1 from Arabidopsis and NTHK1 from tobacco (Nicotiana tabacum), have been found to have His kinase (HK) activity and Ser/Thr kinase activity, respectively, although both show similarity to bacterial two-component HK. Here, we report the characterization of another ethylene receptor homolog gene, NTHK2, from tobacco. This gene also encodes a HK-like protein and is induced by dehydration and CaCl(2) but not significantly affected by NaCl and abscisic acid treatments. The biochemical properties of the yeast (Schizosaccharomyces pombe)-expressed NTHK2 domains were further characterized. We found that NTHK2 possessed Ser/Thr kinase activity in the presence of Mn(2+) and had HK activity in the presence of Ca(2+). Several lines of evidence supported this conclusion, including hydrolytic stability, phosphoamino acid analysis, mutation, deletion, and substrate analysis. These properties have implications in elucidation of the complexity of the ethylene signal transduction pathway and understanding of ethylene functions in plants.  相似文献
5.
A putative ethylene receptor gene NTHK1 encodes a protein with a putative signal peptide, three transmembrane segments, a putative histidine kinase domain and a putative receiver domain. The receiver domain was expressed in an Escherichia coli expression system, purified and used to generate polyclonal antibodies for immunohistochemistry analysis. The spatial expression of the NTHK1 protein was then investigated. We found that NTHK1 was abundant during flower and ovule development. It was also expressed in glandular hairs, stem, and in leaves that had been wounded. The NTHK1 gene was further introduced into the tobacco plant and we found that, in different transgenic lines, the NTHK1 gene was transcribed to various degrees. Upon ACC treatment, the etiolated transgenic seedlings showed reduced ethylene sensitivity when compared with the control, indicating that NTHK1 is a functional ethylene receptor in plants.  相似文献
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改良提取水稻胚乳和拟南芥花柱中DNA和RNA的SDS法   总被引:6,自引:0,他引:6  
在冷酚法的基础上,经多次实践改进,得出一种简单、快速的SDS法,可以从富含多糖的水稻胚乳和富含多酚的拟南芥花柱中同时提取总DNA和总RNA,所得DNA和RNA样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性,并可进一步满足PCR和RT-PCR的实验需要。  相似文献
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土壤动物研究综述   总被引:3,自引:0,他引:3  
土壤动物是当今生态学研究的热点,其对环境的指示作用已经受到越来越多生态学家的重视.对土壤动物学的发展历史,以及我国土壤动物的研究现状和现代土壤动物学研究的一些热点领域进行了综述.  相似文献
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精子肽的固相合成及应用初探   总被引:3,自引:1,他引:2       下载免费PDF全文
目的 :探讨将多肽固相合成技术用于检测抗精子抗体的ELISA试剂盒制备。方法 :以多肽固相合成法合成特异性精子肽 ,并经高效液相纯化分析及质谱分析。以此合成精子肽包板制备检测抗精子抗体的ELISA试剂盒 ,检测血清标本的AsAb。结果 :HPLC结果显示 ,合成的精子肽纯度达 98.26%;质谱分析结果主峰分子质量与理论值一致。采用合成多肽抗原建立了检测抗精子抗体的酶联免疫吸附测定方法 ;不明原因不育患者组与对照组间AsAb发生率呈非常显著差异(P <0,005 )。结论 :本固相合成法可获得高纯度特异性精子肽 ;该精子肽包板的ELISA试剂盒可靠简便。  相似文献
10.
张巍  张志罡  付秀芹  刘立军  颜亨梅 《昆虫学报》2008,51(10):1022-1027
 用转Cry1Ab/Cry1Ac基因水稻的叶片饲喂稻纵卷叶螟Cnaphalocrocis medinalis (Guenée)幼虫,采用酶活力测定方法研究了转基因水稻对稻纵卷叶螟幼虫体内3种保护酶(SOD,CAT和POD)活性的影响。结果表明:取食转基因水稻叶片4 h后,幼虫体内SOD酶活性比对照提高了91.5%,与对照有显著差异;36 h后活性达到最大值,但与对照差异不显著。取食转基因水稻叶片24 h后,幼虫体内CAT酶活性达到最大值且高于对照,但与对照差异不显著;48 h后酶活性受到显著抑制,与对照差异显著。取食转基因水稻叶片12 h后,幼虫体内POD酶活性达到最大值,与对照差异不显著;48 h后酶活性逐渐下降达到最小值,比对照下降68.05%。实验同时测定了幼虫体内及其粪便中Bt毒蛋白含量的变化,结果表明取食转基因水稻叶片12 h后,随着大量取食进入体内的毒蛋白随粪便排出,幼虫体内的毒蛋白含量一直低于粪便中的含量,且在24 h时两者差异达到最大。由于Bt毒蛋白在幼虫体内的积累,扰乱了稻纵卷叶螟幼虫体内SOD,CAT和POD 3种保护酶的动态平衡,使虫体内自由基的清除遇到障碍,从而对其产生毒害作用。  相似文献
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