全文获取类型
收费全文 | 549篇 |
免费 | 40篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 9篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 13篇 |
2015年 | 10篇 |
2014年 | 21篇 |
2013年 | 31篇 |
2012年 | 43篇 |
2011年 | 47篇 |
2010年 | 36篇 |
2009年 | 28篇 |
2008年 | 27篇 |
2007年 | 39篇 |
2006年 | 41篇 |
2005年 | 42篇 |
2004年 | 30篇 |
2003年 | 35篇 |
2002年 | 28篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 6篇 |
1997年 | 6篇 |
1996年 | 7篇 |
1995年 | 11篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1974年 | 2篇 |
1969年 | 1篇 |
1964年 | 1篇 |
1961年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有589条查询结果,搜索用时 15 毫秒
1.
Maxime Bruto Claire Prigent-Combaret Patricia Luis Yvan Mo?nne-Loccoz Daniel Muller 《Proceedings. Biological sciences / The Royal Society》2014,281(1789)
Even genetically distant prokaryotes can exchange genes between them, and these horizontal gene transfer events play a central role in adaptation and evolution. While this was long thought to be restricted to prokaryotes, certain eukaryotes have acquired genes of bacterial origin. However, gene acquisitions in eukaryotes are thought to be much less important in magnitude than in prokaryotes. Here, we describe the complex evolutionary history of a bacterial catabolic gene that has been transferred repeatedly from different bacterial phyla to stramenopiles and fungi. Indeed, phylogenomic analysis pointed to multiple acquisitions of the gene in these filamentous eukaryotes—as many as 15 different events for 65 microeukaryotes. Furthermore, once transferred, this gene acquired introns and was found expressed in mRNA databases for most recipients. Our results show that effective inter-domain transfers and subsequent adaptation of a prokaryotic gene in eukaryotic cells can happen at an unprecedented magnitude. 相似文献
2.
3.
Dimitra Gkika Loic Lemonnier George Shapovalov Dmitri Gordienko Céline Poux Michela Bernardini Alexandre Bokhobza Gabriel Bidaux Cindy Degerny Kathye Verreman Basma Guarmit Mohamed Benahmed Yvan de Launoit Rene J.M. Bindels Alessandra Fiorio Pla Natalia Prevarskaya 《The Journal of cell biology》2015,208(1):89-107
TRPM8 is a cold sensor that is highly expressed in the prostate as well as in other non-temperature-sensing organs, and is regulated by downstream receptor–activated signaling pathways. However, little is known about the intracellular proteins necessary for channel function. Here, we identify two previously unknown proteins, which we have named “TRP channel–associated factors” (TCAFs), as new TRPM8 partner proteins, and we demonstrate that they are necessary for channel function. TCAF1 and TCAF2 both bind to the TRPM8 channel and promote its trafficking to the cell surface. However, they exert opposing effects on TRPM8 gating properties. Functional interaction of TCAF1/TRPM8 also leads to a reduction in both the speed and directionality of migration of prostate cancer cells, which is consistent with an observed loss of expression of TCAF1 in metastatic human specimens, whereas TCAF2 promotes migration. The identification of TCAFs introduces a novel mechanism for modulation of TRPM8 channel activity. 相似文献
4.
Researchers have often assumed that scent marking serves a territorial function in callitrichines, although some controversy
exists. To fulfill such a function, scent marks should 1) prevent intrusions, 2) ensure access to feeding resources, 3) enable
avoidance of intergroup encounters, or 4) play an important role in the aggressive encounters between groups. We studied 13
saddleback tamarins (Saguinus fuscicollis) belonging to 3 free-ranging groups, which formed mixed-species troops with moustached tamarins (S. mystax) in the Amazonian rain forest of Peru. None of the predictions were confirmed. The tamarins used a border-marking strategy,
marking more on the periphery of their territory. However, feeding trees in overlap and encounter areas received more scent
marking but were still visited by neighboring groups. Intergroup encounters occurred more often than expected, and scent-marking
frequency was not higher during them than when no other group was present. It appears that instead of defending a territory
in the classic sense, the tamarins are optimizing signal transmission by depositing their scents where the probability of
detection by neighbors is higher. Saddleback tamarins may use shared areas of their home ranges to exchange information with
neighboring groups, perhaps regarding reproductive opportunities. 相似文献
5.
Francisco J Azuaje Haiying Wang Huiru Zheng Frédérique Léonard Magali Rolland-Turner Lu Zhang Yvan Devaux Daniel R Wagner 《BMC systems biology》2011,5(1):46
Background
Endothelial progenitor cells (EPCs) have been implicated in different processes crucial to vasculature repair, which may offer the basis for new therapeutic strategies in cardiovascular disease. Despite advances facilitated by functional genomics, there is a lack of systems-level understanding of treatment response mechanisms of EPCs. In this research we aimed to characterize the EPCs response to adenosine (Ado), a cardioprotective factor, based on the systems-level integration of gene expression data and prior functional knowledge. Specifically, we set out to identify novel biosignatures of Ado-treatment response in EPCs. 相似文献6.
Sophie Reuse Miriam Calao Kabamba Kabeya Allan Guiguen Jean-Stéphane Gatot Vincent Quivy Caroline Vanhulle Aurélia Lamine Dolores Vaira Dominique Demonte Valérie Martinelli Emmanuelle Veithen Thomas Cherrier Véronique Avettand Solène Poutrel Jacques Piette Yvan de Launoit Michel Moutschen Arsène Burny Christine Rouzioux Stéphane De Wit Georges Herbein Olivier Rohr Yves Collette Olivier Lambotte Nathan Clumeck Carine Van Lint 《PloS one》2009,4(6)
7.
Yvan Zivanovic Jean Armengaud Arnaud Lagorce Christophe Leplat Philippe Guérin Murielle Dutertre Véronique Anthouard Patrick Forterre Patrick Wincker Fabrice Confalonieri 《Genome biology》2009,10(6):R70-23
Background
Thermococcus gammatolerans was isolated from samples collected from hydrothermal chimneys. It is one of the most radioresistant organisms known amongst the Archaea. We report the determination and annotation of its complete genome sequence, its comparison with other Thermococcales genomes, and a proteomic analysis. 相似文献8.
Arnaud Augert Christine Payré Yvan de Launoit Jesus Gil Gérard Lambeau David Bernard 《EMBO reports》2009,10(3):271-277
Senescence is a stable proliferative arrest induced by various stresses such as telomere erosion, oncogenic or oxidative stress. Compelling evidence suggests that it acts as a barrier against tumour development. Describing new mechanisms that favour an escape from senescence can thus reveal new insights into tumorigenesis. To identify new genes controlling the senescence programme, we performed a loss‐of‐function genetic screen in primary human fibroblasts. We report that knockdown of the M‐type receptor PLA2R (phospholipase A2 receptor) prevents the onset of replicative senescence and diminishes stress‐induced senescence. Interestingly, expression of PLA2R increases during replicative senescence, and its ectopic expression results in premature senescence. We show that PLA2R regulates senescence in a reactive oxygen species–DNA damage–p53‐dependent manner. Taken together, our study identifies PLA2R as a potential new tumour suppressor gene crucial in the induction of cellular senescence through the activation of the p53 pathway. 相似文献
9.
The extremely important use of mobile phones in the world, at all ages of life, including children and adolescents, leads to significant exposure of these populations to electromagnetic waves of radiofrequency. The question, therefore, arises as to whether exposure to these radiofrequencies (RFs) could lead to deleterious effects on the body's biological systems and health. In the current article, we review the effects, in laboratory animals and humans, of exposure to RF on two hormones considered as endocrine markers: melatonin, a neurohormone produced by the pineal gland and cortisol, a glucocorticosteroid synthesized by the adrenal glands. These two hormones are also considered as markers of the circadian system. The literature search was performed using PubMed, Medline, Web of Sciences (ISI Web of Knowledge), Google Scholar, and EMF Portal. From this review on RF effects on cortisol and melatonin, it appears that scientific papers in the literature are conflicting, showing effects, no effects, or inconclusive data. This implies the need for additional research on higher numbers of subjects and with protocols perfectly controlled with follow‐up studies to better determine whether the chronic effect of RF on the biological functioning and health of users exists (or not). Bioelectromagnetics. 2021;42:5–17. © 2020 Bioelectromagnetics Society 相似文献
10.
Roque d’orbcastel Emmanuelle Bettarel Yvan Dellinger Marion Sadoul Bastien Bouvier Thierry Amandé Justin Monin Dagorn Laurent Geffroy Benjamin 《Environmental Biology of Fishes》2021,104(6):725-732
Environmental Biology of Fishes - Cortisol is recognized as a physiological indicator of stress in fish. However, this hormone is typically measured in plasma samples. In this study, cortisol... 相似文献