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排序方式: 共有307条查询结果,搜索用时 15 毫秒
1.
Toshihide Hirao Masashi Murakami Jiro Iwamoto Hino Takafumi Hiroyuki Oguma 《Ecological Research》2008,23(1):189-196
The effect of disturbance on local communities may operate within the context of the spatial landscape. We examined the scale-dependent
effects of windthrow disturbance caused by a large typhoon on three arthropod communities in a temperate forest of Japan.
Canopy arthropods were collected by beating foliage, forest-floor arthropods were collected by sweeping the vegetation, and
flying arthropods were collected in Malaise traps. To assess the “functional spatial scale” at which arthropods responded
to tree-fall disturbance, the gap rate was quantified at different spatial scales by sequentially enlarging the radius of
a circular landscape sector in 10-m increments from 10 to 500 m. We then analyzed the responses of order richness and abundance
to the gap rate for each arthropod community. The spatial scale of the significant best-fitting model, which was selected
from the models fitted to the gap rate at stepwise spatial scales, was regarded as the arthropod-specific functional spatial
scale. Arthropod order richness was not dependent on the gap rate. In contrast, arthropod order abundance depended significantly
on the gap rate in many orders, but varied in the response direction and functional spatial scale. These order-specific, scale-dependent
responses to tree-fall gaps could complicate interactions among organisms, leading to complex community organization. An understanding
of the spatial processes that link the use of space by organisms with the spatial scale at which ecological processes are
experienced is required to elucidate the responses of populations, communities, and biotic interactions to disturbances in
a spatial landscape context. 相似文献
2.
A triple helical polysaccharide schizophyllan in aqueous solution exhibited a highly cooperative transition between ordered and disordered states associated with the conformation of its side chains and nearby water molecules. The transition was followed by optical rotation and calorimetry using water containing additives such as NaOH and DMSO as solvents. The ordered state was stabilized or destabilized depending on the kind and amount of the additive employed; in particular, the addition of DMSO had a remarkable stabilizing effect. This effect was analyzed by means of a statistical mechanical theory of linear cooperative transitions, where DMSO was assumed to interact favorably with the ordered side chains. A small amount of NaOH in a solvent mixture stabilized the ordered state and made the transition curve very gradual. No molecular mechanism was elucidated to account for the role of NaOH. 相似文献
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5.
Synthetic oligodeoxyribonucleotides showing abnormal mobilities on polyacrylamide gel electrophoresis 总被引:3,自引:0,他引:3
We found that synthetic DNA fragments containing a GCGAAAGC sequence showed higher mobilities than oligonucleotides without the sequence on denaturing polyacrylamide gel electrophoresis. For example, the fragment, GCGAAAGCT (9mer), showed higher mobility than the corresponding 8mer (CGAAAGCT). In addition, on Maxam-Gilbert sequencing, a 21mer containing the GCGAAAGC sequence showed an abnormal pattern, which were similar to those due to compression observed on sequencing of DNAs with high GC contents, as recently reported. It was suggested that this compression was due to the increased mobilities of the specific fragments with the GCGAAAGC sequence and that these fragments took on abnormal conformations. 相似文献
6.
Parasporin 1Ac2, a Novel Cytotoxic Crystal Protein Isolated from Bacillus thuringiensis B0462 Strain
Shouta Kuroda Anowara Begum Mizue Saga Akina Hirao Eiichi Mizuki Hiroshi Sakai Tohru Hayakawa 《Current microbiology》2013,66(5):475-480
Two novel parasporin (PS) genes were cloned from Bacillus thuringiensis B0462 strain. One was 100 % identical even in nucleotide sequence level with that of parasporin-1Aa (PS1Aa1) from B. thuringiensis A1190 strain. The other (PS1Ac2) showed significant homology (99 % identity) to that of PS1Ac1 from B. thuringiensis 87-29 strain. The 15 kDa (S113–R250) and 60 kDa (I251–S777) fragments consisting of an active form of PS1Ac2 were expressed as His-tag fusion. Upon purification under denaturing condition and refolding, the recombinant polypeptides were applied to cancer cells to analyze their cytotoxicities. 3-(4,5-Dimethyl-2-thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay revealed that either of 15 or 60 kDa polypeptide exhibited no cytotoxicity to HeLa cells, but they became cytotoxic upon mixed together. Our results suggested that PS1Ac2 was responsible for the cytotoxicity of B. thuringiensis B0462 strain, and that the formation of hetero-dimer of 15 and 60 kDa polypeptide was required for their cytotoxicity. 相似文献
7.
A wood-destroying fungus, Trametes sanguinea, produced a potent acid protease in a submerged culture. Maximum proteolytic activity of the culture was attained after 140-hours cultivation in a medium containing dextrin and corn steep liquor. The acid protease was obtained in crystalline form from the mycelium-free culture filtrate by the following successive treatments: acetone precipitation, ionexchange column chromatography, ammonium sulfate fractionation, dialysis, and crystallization by acetone. Throughout the over-all process, the acid protease was purified approximately 30-fold with about 8% recovery of the original activity. 相似文献
8.
Chemical Conversion of Some Ribonucleosides into the Corresponding β-D-Arabinofuranosyl Derivatives1
Nobuo Sakairi Ichiro Hirao Yoshiaki Zama Yoshiharu Ishido 《Nucleosides, nucleotides & nucleic acids》2013,32(3):221-229
Abstract Five 3′,5′-di-O-acylribonucleosides were converted into the corresponding β-D-arabinofuranosyl derivatives through DMSO-oxidation followed by NaBH4-reduction and deacylation with NaOMe-MeOH. 相似文献
9.
Naoki Yokokawa Emi Kikuchi‐Uehara Eri Amasawa Hirokazu Sugiyama Masahiko Hirao 《Journal of Industrial Ecology》2019,23(5):1253-1263
This study analyzed the environmental impacts of packaging‐derived changes in food production and consumer behavior to assist packaging designers in making environmentally conscious decisions. Packaging can be functionalized to prevent food loss and waste (FLW), for example, extending the expiration date and apportioning the package size, but it can generate additional environmental impacts from changes in food and packaging production. Previous studies assessed additional impacts from packaging production; however, the effects of packaging functionalization are yet to be connected with food production and consumer behavior. To examine the effect of functionalization on these aspects, we analyzed packaging‐derived changes in food production for milk and cabbage products. The case study compared products with functionalized packaging that permits a longer expiration date or a smaller portion size to their base‐case products. Our results showed that the packaging‐derived changes increased the global warming potential (GWP) of food production more than other processes did. Thus, changes in food production weakened the effectiveness of the packaging functionalization to decrease the GWP. Moreover, the analysis of consumer behavior scenarios showed that consumers’ perception of the expiration date decisively influences the effectiveness of packaging functionalization. When consumers discarded food after the expiration date, provided they consumed in small quantities, the packaging functionalization reduced FLW. From the scenario analysis, we identified appropriate combinations of packaging functionalization and consumer behaviors to effectively decrease total GWP. With our expanded analysis, packaging designers can understand the effectiveness of their decisions on the product life cycle in reducing FLW and environmental impacts. 相似文献
10.
Shohei Takase Kota Kera Yuya Hirao Tsutomu Hosouchi Yuki Kotake Yoshiki Nagashima 《Bioscience, biotechnology, and biochemistry》2019,83(2):251-261
Cucurbitaceae plants contain characteristic triterpenoids. Momordica charantia, known as a bitter melon, contains cucurbitacins and multiflorane type triterpenes, which confer bitter tasting and exhibit pharmacological activities. Their carbon skeletons are biosynthesized from 2,3-oxidosqualene by responsible oxidosqualene cyclase (OSC). In order to identify OSCs in M. charantia, RNA-seq analysis was carried out from ten different tissues. The functional analysis of the resulting four OSC genes revealed that they were cucurbitadienol synthase (McCBS), isomultiflorenol synthase (McIMS), β-amyrin synthase (McBAS) and cycloartenol synthase (McCAS), respectively. Their distinct expression patterns based on RPKM values and quantitative RT-PCR suggested how the characteristic triterpenoids were biosynthesized in each tissue. Although cucurbitacins were finally accumulated in fruits, McCBS showed highest expression in leaves indicating that the early step of cucurbitacins biosynthesis takes place in leaves, but not in fruits.
Abbreviations: OSC: oxidosqualene cyclase; RPKM: reads perkilobase of exon per million mapped reads 相似文献