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1.
We have developed an experimental system in which the irradiation with a red light pulse induces stomatal disorientation in
the hypocotyl epidermis ofCucumis sativus L. In this system, the orientation of the division plane in guard mother cells was not defined correctly. Preprophase bands
formed in these cells but their orientation was abnormal. 相似文献
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3.
Yoshinobu Hoashi 《Mycoscience》2008,49(6):385-387
Psathyrella turcosomarginata is described and illustrated as a new species from Japan (Fukuoka Prefecture, Kyushu). This species is assigned to section
Spintrigerae because of its lack of pleurocystidia and basidiome habit. It is characterized by having fairly large basidiomes, a distinct
sulcate-striate pileal margin, and cheilocystidia with mucoid deposits that stain bluish green in ammonia solution. 相似文献
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Bacteriorhodopsin (bR) is the prototype of an integral membrane protein with seven membrane-spanning α-helices and serves as a model of the G-protein-coupled drug receptors. This study is aimed at reaching a greater understanding of the role of amine local anesthetic cations on the proton transport in the bR protein, and furthermore, the functional role of “the cation” in the proton pumping mechanism. The effect of the amine anesthetic cations on the proton pump in the bR blue membrane was compared with those by divalent (Ca2+, Mg2+ and Mn2+) and monovalent metal cations (Li+, Na+, K+ and Cs+), which are essential for the correct functioning of the proton pumping of the bR protein. The results suggest that the interacting site of the divalent cation to the bR membrane may differ from that of the monovalent metal cation. The electric current profile of the bR blue membrane in the presence of the amine anesthetic cations was biphasic, involving the generation and inhibition of the proton pumping activity in a concentration-dependent manner. The extent of the regeneration of the proton pump by the additives increased in the order of monovalent metal cation<monovalent amine anesthetic cation<divalent metal cation. We found that organic cations such as the amine anesthetics can also regenerate the proton pump in the bR protein. The inhibition of proton transport in the bR protein by the anesthetic cations was elucidated using the wild type, the E204Q and the D96N mutated bRs. The hydrophobic interaction of the amine anesthetics with the bR protein plays an important part in inhibiting the bR proton pump. 相似文献
6.
Naohiro Sakata Yoshinobu Hoshii Tomomi Nakamura Makiko Kiyama Hirofumi Arai Masatoshi Omoto Mitsunori Morimatsu Tokuhiro Ishihara 《The journal of histochemistry and cytochemistry》2005,53(2):237-242
Apolipoprotein AI (apoAI), a major component of high-density lipoproteins, is one of the major amyloid fibril proteins and a minor constituent of the senile plaques observed in Alzheimer's disease. We examined colocalization of apoAI in various kinds of systemic amyloidosis in this study. Forty-three of 48 formalin-fixed paraffin-embedded heart specimens with various forms of systemic amyloidosis reacted immunohistochemically with anti-human apoAI antibody. ApoAI was also detected in water-extracted amyloid material by immunoblotting. In addition, we observed colocalization of apoAI and murine amyloid A (AA) amyloidosis in human apoAI transgenic mice. This is the first report of colocalization of apoAI with amyloid deposits in various forms of human systemic amyloidosis and murine AA amyloidosis in human apoAI transgenic mice. ApoAI may not always be a major component of amyloid fibrils, even when it is present in systemic amyloid deposits. 相似文献
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Tadahiko Kajiwara Yoshinobu Odake Akikazu Hatanaka 《Bioscience, biotechnology, and biochemistry》2013,77(8):1617-1621
3Z-Nonenal and 3Z, 6Z-nonadienal, potential biosynthetic precursors of 2E-nonenal and 2E, 6Z-nonadienal, were for the first time synthesized stereoseleclively. 相似文献
9.
Effect of Materials Released from Myofibrils by CAF (Ca2+-activated Factor) on Z-Disk Reconstitution
Atsushi Suzuki Makoto Saito Hiroshi Sato Yoshinobu Nonami 《Bioscience, biotechnology, and biochemistry》2013,77(11):2111-2116
To investigate the constituents of Z-disk, reconstitution of Z-disk by incubating some proteins released from myofibrils by CAF(Ca2+-activated factor) with Z-disk-extracted fiber bundles was carried out and examined with electron microscope. The materials released from myofibrils by CAF have been bound in Z-disk region, and Z-disk extracted from myofibrils with a low ionic strength solution has been reconstituted. On the other hand, Z-disk removed from myofibrils by CAF has not been reconstituted by the same way. 相似文献
10.
Miyagawa Eiji Junko Yano Toshinari Hamakado Yasuji Kido Keiji Nishimoto Yoshinobu Motoki 《Bioscience, biotechnology, and biochemistry》2013,77(10):2881-2886
N-Benzoylgiycine amidohydrolase (hippurate hydrolase EC 3.5.1.32), which catalyzes the hydrolysis of hippuric acid to benzoic acid and glycine, was found in a cell-free extract of Pseudomonas putida C692-3 grown on a medium containing hippuric acid. The enzyme was purified from the extract by ammonium sulfate fractionation and column chromatographies on DEAE-cellulose, DEAE-Sephadex A-50, hydroxyapatite, and Sepharose CL-6B. The enzyme was finally crystallized. The crystalline enzyme was almost homogeneous on electrophoresis. The enzyme had a molecular weight of about 170,000 and consisted of four subunits identical in molecular weight (approximately 42,000). The enzyme hydrolyzed N-benzoylglycine most rapidly, and N-benzoyl-l-alanine and N-benzoyl-l-aminobutyric acid. The Km value for these substrates were 0.72 mm, 0.87 mm, and 0.87mm, respectively. The optimum pH of the enzyme reaction was 7.0 to 8.0 and the enzyme was stable from pH 6.0 to 8.0. 相似文献