全文获取类型
收费全文 | 85747篇 |
免费 | 20442篇 |
国内免费 | 6081篇 |
出版年
2024年 | 40篇 |
2023年 | 764篇 |
2022年 | 1033篇 |
2021年 | 3224篇 |
2020年 | 4107篇 |
2019年 | 6111篇 |
2018年 | 5979篇 |
2017年 | 5763篇 |
2016年 | 6400篇 |
2015年 | 7601篇 |
2014年 | 8101篇 |
2013年 | 8958篇 |
2012年 | 7656篇 |
2011年 | 6809篇 |
2010年 | 6311篇 |
2009年 | 4712篇 |
2008年 | 4389篇 |
2007年 | 3501篇 |
2006年 | 3172篇 |
2005年 | 2751篇 |
2004年 | 2519篇 |
2003年 | 2308篇 |
2002年 | 2070篇 |
2001年 | 1352篇 |
2000年 | 1087篇 |
1999年 | 875篇 |
1998年 | 584篇 |
1997年 | 501篇 |
1996年 | 493篇 |
1995年 | 474篇 |
1994年 | 442篇 |
1993年 | 310篇 |
1992年 | 397篇 |
1991年 | 271篇 |
1990年 | 213篇 |
1989年 | 221篇 |
1988年 | 144篇 |
1987年 | 127篇 |
1986年 | 102篇 |
1985年 | 118篇 |
1984年 | 47篇 |
1983年 | 54篇 |
1982年 | 51篇 |
1981年 | 22篇 |
1980年 | 14篇 |
1979年 | 16篇 |
1977年 | 7篇 |
1973年 | 5篇 |
1971年 | 5篇 |
1962年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Single Cell Transcriptome Amplification with MALBAC 总被引:1,自引:0,他引:1
2.
3.
ObjectiveWe investigated whether glutamate, NMDA receptors, and eukaryote elongation factor-2 kinase (eEF-2K)/eEF-2 regulate P-glycoprotein expression, and the effects of the eEF-2K inhibitor NH125 on the expression of P-glycoprotein in rat brain microvessel endothelial cells (RBMECs).MethodsCortex was obtained from newborn Wistar rat brains. After surface vessels and meninges were removed, the pellet containing microvessels was resuspended and incubated at 37°C in culture medium. Cell viability was assessed by the MTT assay. RBMECs were identified by immunohistochemistry with anti-vWF. P-glycoprotein, phospho-eEF-2, and eEF-2 expression were determined by western blot analysis. Mdr1a gene expression was analyzed by RT-PCR.ResultsMdr1a mRNA, P-glycoprotein and phospho-eEF-2 expression increased in L-glutamate stimulated RBMECs. P-glycoprotein and phospho-eEF-2 expression were down-regulated after NH125 treatment in L-glutamate stimulated RBMECs.ConclusionseEF-2K/eEF-2 should have played an important role in the regulation of P-glycoprotein expression in RBMECs. eEF-2K inhibitor NH125 could serve as an efficacious anti-multidrug resistant agent. 相似文献
4.
Michael P. Gustafson Yi Lin Mary L. Maas Virginia P. Van Keulen Patrick B. Johnston Tobias Peikert Dennis A. Gastineau Allan B. Dietz 《PloS one》2015,10(3)
The development of flow cytometric biomarkers in human studies and clinical trials has been slowed by inconsistent sample processing, use of cell surface markers, and reporting of immunophenotypes. Additionally, the function(s) of distinct cell types as biomarkers cannot be accurately defined without the proper identification of homogeneous populations. As such, we developed a method for the identification and analysis of human leukocyte populations by the use of eight 10-color flow cytometric protocols in combination with novel software analyses. This method utilizes un-manipulated biological sample preparation that allows for the direct quantitation of leukocytes and non-overlapping immunophenotypes. We specifically designed myeloid protocols that enable us to define distinct phenotypes that include mature monocytes, granulocytes, circulating dendritic cells, immature myeloid cells, and myeloid derived suppressor cells (MDSCs). We also identified CD123 as an additional distinguishing marker for the phenotypic characterization of immature LIN-CD33+HLA-DR- MDSCs. Our approach permits the comprehensive analysis of all peripheral blood leukocytes and yields data that is highly amenable for standardization across inter-laboratory comparisons for human studies. 相似文献
5.
6.
Detection and genotyping of group A rotaviruses isolated from sewage samples in Monastir,Tunisia between April 2007 and April 2010 下载免费PDF全文
7.
8.
9.