全文获取类型
收费全文 | 1463篇 |
免费 | 192篇 |
国内免费 | 2篇 |
出版年
2023年 | 7篇 |
2021年 | 27篇 |
2020年 | 15篇 |
2019年 | 21篇 |
2018年 | 32篇 |
2017年 | 33篇 |
2016年 | 42篇 |
2015年 | 80篇 |
2014年 | 86篇 |
2013年 | 104篇 |
2012年 | 121篇 |
2011年 | 117篇 |
2010年 | 70篇 |
2009年 | 52篇 |
2008年 | 71篇 |
2007年 | 68篇 |
2006年 | 72篇 |
2005年 | 68篇 |
2004年 | 62篇 |
2003年 | 58篇 |
2002年 | 46篇 |
2001年 | 25篇 |
2000年 | 41篇 |
1999年 | 22篇 |
1998年 | 13篇 |
1997年 | 15篇 |
1996年 | 12篇 |
1995年 | 12篇 |
1994年 | 11篇 |
1993年 | 13篇 |
1992年 | 17篇 |
1991年 | 23篇 |
1990年 | 9篇 |
1989年 | 23篇 |
1988年 | 19篇 |
1987年 | 14篇 |
1986年 | 10篇 |
1985年 | 16篇 |
1984年 | 10篇 |
1983年 | 12篇 |
1982年 | 9篇 |
1981年 | 10篇 |
1980年 | 6篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1976年 | 5篇 |
1974年 | 4篇 |
1973年 | 6篇 |
1971年 | 4篇 |
1968年 | 4篇 |
排序方式: 共有1657条查询结果,搜索用时 0 毫秒
1.
2.
Myeong Hee Kim Yee Hyung Kim So Young Kang Woo In Lee 《Indian journal of microbiology》2015,55(4):464-468
Non-tuberculous mycobacterium (NTM) lung disease is increasing in prevalence. We analyzed the frequency of NTM lung disease among patients who are suspected of tuberculosis. NTM was isolated from about one-fourth of the mycobacterium culture-positive patients and about half of these had NTM lung disease. Therefore, NTM isolates should be routinely identified at the species level for adequate treatment. 相似文献
3.
4.
5.
The binding of chlorpromazine · HCl at the human erythrocyte surface has been detected through its effect on cellular electrophoretic mobility. Incubation of erythrocytes (approx. 5 · 106/ml) in 23 μM chlorpromazine · HCl resulted in a reduction of negative electrophoretic mobility from the control value of (μm · s?1)/(V · cm?1) to (μm · s?1)/(V · cm?1) (pH 7.2, ionic strength 0.155). This mobility change was completely reversed when chlorpromazine · HCl was removed by centrifugal washing. Increasing the drug concentration to 70μM did not affect the mobility change, indicating saturation of the electrophoretically detectable drug binding sites over chlorpromazine · HCl concentration range studied here. The effect of the 23 μM chlorpromazine · HCl on electrophoretic mobility was also measured in isotonic media of reduced ionic strength. The drug-induced reduction in negative surface charge density was found to be independent of ionic strength over the range 0.155 (Debye length, 0.8 nm) to 0.00310 (Debye length, 5.7 nm).Fixation of erythrocytes with glutaraldehyde affected neither the normal electrophoretic mobility of discocytes nor the reduced electrophoretic mobility of chlorpromazine · HCl-induced stomatocytes. When these stomatocytes were first fixed with glutaraldehyde, then washed free of chlorpromazine · HCl, they retained the stomatocyte form while regaining a normal control electrophoretic mobility. Conversely, when discocytes fixed in that form were treated with chlorpromazine · HCl, they showed the same mobility change as did fixed or unfixed stomatocytes. The drug-induced mobility change is therefore independent of the shape change, but reflects a contribution to cellular surface charge density from the membrane-bound chlorpromazine · HCl molecules. From the charge reduction, it is estimated that about 106 chlorpromazine · HCl molecules are bound at the electrokinetic cell surface and occupy approximately 0.4% of the total surface area. 相似文献
6.
Alistair G. S. Philip Ann B. Yee Moothedan Rosy Nergesh Surti A. Tsamtsouris David Ingall 《BMJ (Clinical research ed.)》1969,2(5648):11
The details of the deliveries of 10 infants whose cords were clamped before the onset of respiration and within one minute of delivery of the chin but whose residual placental volumes were unexpectedly low are compared with 20 control infants whose cords were clamped under similar conditions but who had the expected residual placental volumes. The only statistically significant difference between these groups was in the high number of patients with foetal distress and low Apgar scores in the former group. It is concluded that placental transfusion occurred before delivery in these patients and that foetal asphyxia facilitated this transfusion, which may be the underlying mechanism of neonatal erythrocythaemia or transient tachypnoea of the newborn. 相似文献
7.
8.
9.
The distribution of d(CT)-rich pyrimidine tracts in the karyotypes of a variety of vertebrates was studied by in situ hybridization. The probe for these studies was a 56 bp homopyrimidine/homopurine sequence obtained from a mouse genomic library constructed with DNA prepared from a restriction enzyme digestion of metaphase chromosomes. Single-stranded DNA nuclease digestions and two-dimensional gel analysis of topoisomers of this sequence indicated that it is capable of adopting a triplex conformation in vitro. In situ hybridization with this probe to the karyotypes of ten different vertebrate species revealed a highly conserved chromosomal distribution of d(CT)-rich tracts. These tracts are found throughout the chromosomal arms and in some karyotypes they are clustered, producing a banding pattern. However, at the resolution of the light microscope these tracts appeared to be absent from the centromeric regions of all chromosomes examined except those of chicken. The non-random distribution of these tracts to the chromosomal arm regions implies an organizational or functional role for this repeat class. It is unlikely that the 56 bp sequence type contributed to the formation of the triplex DNA structure previously detected in centromeric domains of mouse. 相似文献
10.
HemoHIM, an herbal preparation of three edible herbs (Angelica gigas Nakai, Cnidium officinale Makino, Paeonia japonica Miyabe) is known to increase the Th1 immune response as well as reduce the allergic response in human mast cells. Here, our goal was to determine whether or not HemoHIM could induce Th1 cell differentiation as well as inhibit the development of airway inflammation. To study Th1/Th2 cell differentiation, naive CD4+ T cells isolated from C57BL/6 mouse spleens were cultured with or without HemoHIM. To examine airway inflammation, C57BL/6 mice were fed HemoHIM for 4 weeks before sensitization and provocation with ovalbumin (OVA). In an in vitro experiment, naive CD4+ T cells displayed increased Th1 (IFN-γ+ cell) as well as decreased Th2 (IL-4+ cell) differentiation in a HemoHIM concentration-dependent manner. Furthermore, in an airway inflammation mice model, eosinophil numbers in BALF, serum levels of OVA-specific IgE and IgG1, and cytokine (IL-4, IL-5, and IL-13) levels in BALF and the supernatant of splenocytes all decreased upon HemoHIM (100 mg/kg body weight) pretreatment (4 weeks). These results show that HemoHIM attenuated allergic airway inflammation in the mouse model through regulation of the Th1/Th2 balance. 相似文献