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Ha Na Seo Bo Young Jeon Hung Thuan Tran Dae Hee Ahn Doo Hyun Park 《Biotechnology and Bioprocess Engineering》2008,13(2):224-231
Saccharomyces sp. SK0704 (further defined as SK0704) isolated from long-term-ripening kimchi was identified by a biochemical method with
an API kit; its physiology was found to be very similar to that of S. cerevisiae ATCC 26603 (further defined as ATCC 26603), except in terms of starch utilization. SK0704 did not excrete extracellular glucoamylase,
but utilized starch as a sole carbon source under only aerobic conditions. Crude enzyme excreted from SK0704 catalyzed the
saccharification of starch to glucose, but ATCC 26603 did not. The PCR product obtained using the chromosomal DNA of SK0704
and the primers designed on the basis of the extracellular glucoamylase-coding gene of S. diastaticus was homologous with the intracellular sporulation-specific glucoamylase of S. cerevisiae. SDS-PAGE pattern of soluble protein extracted from yeast cells grown on glucose was greatly different from that on starch.
From these results, we proposed that the SK0704 may have a specific physiological function for starch catabolism such as membrane
transport system and intracellular sac-charification of starch. 相似文献
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Abstract The syntheses of three classes of adenosine analogues involving cyclosubstitution at the 6-position and functionalization at the 2-position are reported. The target molecules synthesized are stable with respect to hydrolytic deamination by mammalian adenosine deaminase, and, because of major structural changes at the 2- and 6-positions, these compounds are expected to be poor phosphorylation substrates for the kinases. Adenosine receptor binding data reveal that several of the compounds synthesized show excellent A1 receptor affinity and A2/A1 selectivity. 相似文献
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Hair is often used as an index of environmental and industrial exposure to different metals. The interpretation of metal levels
in hair is difficult because of the risk of external contamination. The aim of this study was to define the degree of external
contamination of hair exposed in vitro to mercury vapor. Specimens of hair were exposed to concentration: 0.026, 0.21, and
2.7 mg Hg/m3 for 2–28 d. Mercury levels in hair increased during 28 d of exposure 2, 3 and 13, times, respectively, when compared to initial
values. Mercury levels in hair exposed to the first and second (but not third) concentration of mercury vapor attained a steady
state on the 21st d of exposure. The contamination of hair with mercury could not be removed by washing with water, solvent,
and detergent.
Hair may be used as an index of internal uptake of mercury provided that it was not externally exposed to mercury vapor. In
cases of occupational exposure to mercury vapor, hair could become a useful tool for monitoring exposures. 相似文献