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1.
Jian-bo Fan Wei LiuKun Yuan Xin-hui ZhuDa-wei Xu Jia-jia ChenZhi-ming Cui 《Biochemical and biophysical research communications》2014
Pleiotrophin (Ptn) plays an important role in bone growth through regulating osteoblasts’ functions. The underlying signaling mechanisms are not fully understood. In the current study, we found that Ptn induced heparin-binding epidermal growth factor (HB-EGF) release to trans-activate EGF-receptor (EGFR) in both primary osteoblasts and osteoblast-like MC3T3-E1 cells. Meanwhile, Ptn activated Akt and Erk signalings in cultured osteoblasts. The EGFR inhibitor AG1478 as well as the monoclonal antibody against HB-EGF (anti-HB-EGF) significantly inhibited Ptn-induced EGFR activation and Akt and Erk phosphorylations in MC3T3-E1 cells and primary osteoblasts. Further, EGFR siRNA depletion or dominant negative mutation suppressed also Akt and Erk activation in MC3T3-E1 cells. Finally, we observed that Ptn increased alkaline phosphatase (ALP) activity and inhibited dexamethasone (Dex)-induced cell death in both MC3T3-E1 cells and primary osteoblasts, such effects were alleviated by AG1478 or anti-HB-EGF. Together, these results suggest that Ptn-induced Akt/Erk activation and some of its pleiotropic functions are mediated by EGFR trans-activation in cultured osteoblasts. 相似文献
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The histone acetyltransferase MOF overexpression blunts cardiac hypertrophy by targeting ROS in mice
Weiwei Qiao Weili Zhang Yusheng GaiLan Zhao Juexin Fan 《Biochemical and biophysical research communications》2014
Imbalance between histone acetylation/deacetylation critically participates in the expression of hypertrophic fetal genes and development of cardiac hypertrophy. While histone deacetylases play dual roles in hypertrophy, current evidence reveals that histone acetyltransferase such as p300 and PCAF act as pro-hypertrophic factors. However, it remains elusive whether some histone acetyltransferases can prevent the development of hypertrophy. Males absent on the first (MOF) is a histone acetyltransferase belonging to the MYST (MOZ, Ybf2/Sas3, Sas2 and TIP60) family. Here in this study, we reported that MOF expression was down-regulated in failing human hearts and hypertrophic murine hearts at protein and mRNA levels. To evaluate the roles of MOF in cardiac hypertrophy, we generated cardiac-specific MOF transgenic mice. MOF transgenic mice did not show any differences from their wide-type littermates at baseline. However, cardiac-specific MOF overexpression protected mice from transverse aortic constriction (TAC)-induced cardiac hypertrophy, with reduced radios of heart weight (HW)/body weight (BW), lung weight/BW and HW/tibia length, decreased left ventricular wall thickness and increased fractional shortening. We also observed lower expression of hypertrophic fetal genes in TAC-challenged MOF transgenic mice compared with that of wide-type mice. Mechanically, MOF overexpression increased the expression of Catalase and MnSOD, which blocked TAC-induced ROS and ROS downstream c-Raf-MEK-ERK pathway that promotes hypertrophy. Taken together, our findings identify a novel anti-hypertrophic role of MOF, and MOF is the first reported anti-hypertrophic histone acetyltransferase. 相似文献
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目的探讨高糖(High glucose,HG)对小鼠海马神经元细胞HT22细胞的生长抑制作用,分析其是否通过HG诱导细胞衰老以及凋亡而实现。方法采用台盼蓝染色计数法检测细胞生长状况并绘制生长曲线,β-半乳糖苷酶(Senescence associated acidic-β-galactosidas,SA-β-Gal)染色法检测衰老的HT22细胞,Hoechst 33258染色法检测HT22细胞凋亡形态。结果(1)HG(13.5、27、40.5mg/ml,48h)能显著抑制HT22细胞生长(P0.05,P0.01);(2)HG(13.5、27、40.5mg/ml)处理48h可明显诱导SA-β-Gal染色阳性率增加(P0.001);(3)HG(13.5、27、40.5mg/ml,48h)能诱导HT22细胞发生凋亡(P0.001),且呈浓度依赖性。结论 HG可通过诱导HT22细胞衰老和凋亡而抑制HT22细胞生长。 相似文献
5.
Laura Goeser Ting-Jia Fan Sandrine Tchaptchet Nikolas Stasulli William E. Goldman R. Balfour Sartor Jonathan J. Hansen 《PloS one》2015,10(3)
Many intracellular bacterial pathogens possess virulence factors that prevent detection and killing by macrophages. However, similar virulence factors in non-pathogenic bacteria are less well-characterized and may contribute to the pathogenesis of chronic inflammatory conditions such as Crohn’s disease. We hypothesize that the small heat shock proteins IbpAB, which have previously been shown to reduce oxidative damage to proteins in vitro and be upregulated in luminal non-pathogenic Escherichia strain NC101 during experimental colitis in vivo, protect commensal E. coli from killing by macrophage-derived reactive oxygen species (ROS). Using real-time PCR, we measured ibpAB expression in commensal E. coli NC101 within wild-type (wt) and ROS-deficient (gp91phox-/-) macrophages and in NC101 treated with the ROS generator paraquat. We also quantified survival of NC101 and isogenic mutants in wt and gp91phox-/- macrophages using gentamicin protection assays. Similar assays were performed using a pathogenic E. coli strain O157:H7. We show that non-pathogenic E. coli NC101inside macrophages upregulate ibpAB within 2 hrs of phagocytosis in a ROS-dependent manner and that ibpAB protect E. coli from killing by macrophage-derived ROS. Moreover, we demonstrate that ROS-induced ibpAB expression is mediated by the small E. coli regulatory RNA, oxyS. IbpAB are not upregulated in pathogenic E. coli O157:H7 and do not affect its survival within macrophages. Together, these findings indicate that ibpAB may be novel virulence factors for certain non-pathogenic E. coli strains. 相似文献
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豫东平原聚落景观格局变化 总被引:4,自引:1,他引:3
中国的快速城镇化必然导致聚落景观格局的变化,但目前相关研究对平原地区关注相对较少。文中选取豫东平原地区的开封、商丘、周口市,运用GIS空间分析方法和ENVI遥感影像处理技术,对该地区1972、2015年市域中观尺度上的聚落规模、聚落景观空间变化特征进行分析。结果显示:(1)在聚落发展过程中,1972—2015年聚落斑块数量有所减少,聚落规模逐渐扩张,市域内最大聚落斑块扩张相对更为明显;对聚落斑块扩张强度的分析发现,城市市辖区及周边乡镇聚落扩张强度相对较高。(2)地区内聚落斑块空间分布表现出聚集分布特征,但该时期聚集程度有所减弱;两个年份聚落核密度分布格局大致相似,局部地区存在多核扩散现象,市域尺度上的核密度分布存在地区差异。(3)对斑块形状指数的分析发现,研究区狭长或曲折聚落斑块在空间上分散布局,整体上区域内聚落形状趋于规则。(4)随着距河流、道路距离的增加,聚落斑块总面积和数量有所减少,且道路对聚落分布仅在一定范围内存在较大影响,距中心城市0—6km范围内聚落受中心城区发展辐射影响较大。本文的分析可为平原地区聚落景观的优化布局和聚落用地的集约化发展提供一定的参考。 相似文献