Ethanol potentiation of GABAA receptors requires phosphorylation of the alternatively spliced variant of the gamma 2 subunit.

The mammalian GABAA receptor is a multisubunit protein containing a variety of binding sites for psychotropic agents. One of the most widely used of these drugs, ethanol, enhances the function of GABAA receptors in certain circumstances but not others. Previous studies have demonstrated that alternative splicing of the gamma 2L GABA subunit results in an ethanol sensitive and an ethanol-insensitive form, when combined with alpha and beta subunits. We have used in vitro mutagenesis and expression in Xenopus oocytes to show that the consensus site for phosphorylation by protein kinase C contained in the gamma 2L insert is critical for modulation by ethanol but not benzodiazepines, and manipulation of the phosphorylating enzymes in oocytes containing alpha 1 beta 1 gamma 2L can prevent ethanol enhancement. It is likely that phosphorylation or dephosphorylation of a specific site on the GABAA receptor protein can act as a control mechanism for neuronal responses to alcohol exposure.     

Hereditary in wasps. A study of heredity in a parthenogenetic insect, the parasite wasp, Hadrobraeon

Ohne Zusammenfassung

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Hereditary in wasps. A study of heredity in a parthenogenetic insect, the parasite wasp, Hadrobraeon

     

Sex-determination and biology of a parasitic wasp, Hadrobracon brevicornis (Wesmael)

Ohne Zusammenfassung

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Sex-determination and biology of a parasitic wasp, Hadrobracon brevicornis (Wesmael)

     

The GABA-A receptor gene family: new targets for therapeutic intervention.

Until 1987, when the first GABA-A receptor subunit cDNAs were cloned and sequenced, it was thought that there were perhaps two subtypes of receptor in the brain. These were defined by the fact that benzodiazepines, which act through the GABA-A receptor, had two binding sites with different affinities. By 1991 it was known that the GABA-A receptor family existed as a family of subunits which coassembled to form a family of receptor subtypes in the brain. More recently, two additional GABA-A receptor subunits have been identified, epsilon and theta. The identification of these new members of the gene family, and the characterisation of the receptor subtypes into which they are incorporated, is reviewed.