全文获取类型
收费全文 | 42155篇 |
免费 | 7014篇 |
国内免费 | 20677篇 |
出版年
2024年 | 156篇 |
2023年 | 1268篇 |
2022年 | 2134篇 |
2021年 | 2455篇 |
2020年 | 2486篇 |
2019年 | 2837篇 |
2018年 | 2009篇 |
2017年 | 1849篇 |
2016年 | 2041篇 |
2015年 | 2713篇 |
2014年 | 3698篇 |
2013年 | 3270篇 |
2012年 | 4445篇 |
2011年 | 4432篇 |
2010年 | 3580篇 |
2009年 | 3568篇 |
2008年 | 3860篇 |
2007年 | 3592篇 |
2006年 | 3385篇 |
2005年 | 2805篇 |
2004年 | 2243篇 |
2003年 | 1935篇 |
2002年 | 1748篇 |
2001年 | 1648篇 |
2000年 | 1447篇 |
1999年 | 947篇 |
1998年 | 532篇 |
1997年 | 361篇 |
1996年 | 302篇 |
1995年 | 261篇 |
1994年 | 190篇 |
1993年 | 182篇 |
1992年 | 176篇 |
1991年 | 165篇 |
1990年 | 132篇 |
1989年 | 112篇 |
1988年 | 121篇 |
1987年 | 91篇 |
1986年 | 86篇 |
1985年 | 78篇 |
1984年 | 65篇 |
1983年 | 53篇 |
1982年 | 89篇 |
1981年 | 45篇 |
1979年 | 20篇 |
1957年 | 22篇 |
1956年 | 12篇 |
1954年 | 12篇 |
1953年 | 13篇 |
1950年 | 21篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
针对抗虫耐除草剂大豆转基因品系MON89788,从转基因植物基因组DNA的提取、核酸模板的质量和浓度控制、引物探针的筛选、PCR反应过程的建立等方面建立了一套完整的转基因大豆芯片式dPCR定量检测方法。本实验也对该方法的重复性和定量检测限进行考察。10组5%转基因品系大豆MON89788样品定量重复性RSD在1.17%-9.97%之间,均满足国际上转基因定量结果RSD小于25%的要求。用该方法对转基因含量为5%、1%、0.1%的大豆MON89788进行定量检测,其定量结果为5.20%、0.94%和0.11%,RSD分别为6.2%、3.6%和15.2%。该检测方法的定量限达到0.1%,能满足欧盟对转基因定量标识0.9%的要求。将本实验建立的方法用于转基因大豆的定量检测,能为规范我国转基因监管工作的实施提供强有力的技术支撑。 相似文献
4.
<正>There is no experiencing without an experiencer,the self.Whether the experiencer knows it is"the self"that is experiencing is a different and tricky philosophical issue.Dating back to Ivan Pavlov’s dogs,the"experience"of animals has 相似文献
5.
Brucella cell surface protein (BCSP31) is potentially useful for diagnosing brucellosis. We aimed to establish a monoclonal antibody
(MAb) against Brucella melitensis BCSP31 and to investigate its distribution in diagnosis. Soluble recombinant BCSP31 was successfully expressed and purified.
Two MAbs (1F1 and 1E5) against B. melitensis BCSP31, effective in detecting both recombinant and cellular proteins, were obtained and characterized. The MAbs did not
react with Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Mycobacterium tuberculosis, or Bacillus aeruginosus, but strongly reacted with BCSP31 and B. melitensis by ELISA and Western blot analysis. We also tested different Brucella species and brucellosis using the prepared anti-BCSP31 MAbs. BCSP31 and anti-BCSP31 MAbs may play important roles in future
research in diagnosing brucellosis. 相似文献
6.
Kimberly R. Holloway Vidya C. Sinha Michael J. Toneff Wen Bu Susan G. Hilsenbeck Yi Li 《PloS one》2015,10(1)
While most breast cancers are thought to arise from the luminal layer of the breast tissue, it remains unclear which specific cells in the luminal layer are the cells of origin of breast cancer. We have previously reported that WAP-positive luminal epithelial cells are at increased susceptibility to tumor initiation by ErbB2 compared to the bulk population, while the mammary cells with canonical Wnt signaling activity fail to evolve into tumors upon ErbB2 activation. Here, we used retrovirus to introduce ErbB2 into the Krt6a-positive mammary progenitor subset of the luminal epithelium and, for comparison, into the mammary luminal epithelium indiscriminately. Tumors developed from both groups of cells with a similar latency. These data indicate that the Krt6a-positive subset of mammary epithelial cells can be induced to form cancer by ErbB2 but it is not more susceptible to tumorigenesis initiated by ErbB2 than the bulk population of the luminal epithelium. 相似文献
7.
Wen-Ching Lin Chih-Chun Wen Yung-Hsiang Chen Pei-Wen Hsiao Jiunn-Wang Liao Ching-I Peng Ning-Sun Yang 《PloS one》2015,10(6)
For the development of “medical foods” and/or botanical drugs as defined USA FDA, clear and systemic characterizations of the taxonomy, index phytochemical components, and the functional or medicinal bioactivities of the reputed or candidate medicinal plant are needed. In this study, we used an integrative approach, including macroscopic and microscopic examination, marker gene analysis, and chemical fingerprinting, to authenticate and validate various species/varieties of Wedelia, a reputed medicinal plant that grows naturally and commonly used in Asian countries. The anti-inflammatory bioactivities of Wedelia extracts were then evaluated in a DSS-induced murine colitis model. Different species/varieties of Wedelia exhibited distinguishable morphology and histological structures. Analysis of the ribosomal DNA internal transcribed spacer (ITS) region revealed significant differences among these plants. Chemical profiling of test Wedelia species demonstrated candidate index compounds and distinguishable secondary metabolites, such as caffeic acid derivatives, which may serve as phytochemical markers or index for quality control and identification of specific Wedelia species. In assessing their effect on treating DSS induced-murine colitis, we observed that only the phytoextract from W. chinensis species exhibited significant anti-inflammatory bioactivity on DSS-induced murine colitis among the various Wedelia species commonly found in Taiwan. Our results provide a translational research approach that may serve as a useful reference platform for biotechnological applications of traditional phytomedicines. Our findings indicate that specific Wedelia species warrant further investigation for potential treatment of human inflammatory bowel disease. 相似文献
8.
9.
Cytosine–guanine (CpG) containing oligodeoxynucleotides (ODN) have significant clinical potential as immunotherapeutics. However, limitations exist due to their transient biological stability in vivo, lack of specificity for target cells, and poor cellular uptake. To address these issues, we prepared amine magnetic mesoporous silica nanoparticles (M-MSN-A) then further modified with polyethylene glycol (PEG) for use as CpG delivery vectors. The PEG modified M-MSN-A (M-MSN-P) had notable CpG ODN loading capacity, negligible cytotoxicity, and were easily internalized into cells where they released the loaded CpG into the cytoplasm. As a result, such complexes were effective in activating macrophages and inhibiting tumor cells when combined with chemotherapeutics in vitro. Furthermore, these complexes had excellent immuno-stimulating activity in vivo, compared to the free CpG therapeutics. We report here a highly effective MSNs-based delivery system with great potential as a therapeutic CpG formulation in cancer immunotherapy. 相似文献
10.