Screen-grid mammography]

A device and the results of a 3-year performance of cellular grids for mammography were described. An increase in the informative value and a decrease in radiation exposure of patients were shown during mammography with a cellular grid.     

The 4-dimensional spherical color space in the monkey]

Discrimination of colours by macaque rhesus was studied by the method of elaboration of differentiation inhibition of instrumental conditioned responses to colour stimuli. Matrix of probabilities of instrumental reactions to presentation of colour stimuli, the columns of which corresponded to the colours applied, and the lines--to series of experiments with definite reinforced colour--was processed by the method of factor analysis. Four factors describing the used colours in four-dimensional Euclidean space were singled out. Spatial structure of the seven used colours satisfies the equation of four-dimensional sphere. Two first factors are interpreted as colour-opponent red-green and yellow-blue and the third and fourth ones as achromatic light and dark neuronal channels. Perceptive space of colour stimuli based on the data of instrumental behaviour of the monkey corresponds to analogous results obtained by the method of multidimensional scaling of subjective evaluations of super-threshold colour differences for the man.     

The effect of x-ray radiation and hypoxia on the proteolytic activity of the normal rat spleen and during tumor growth

The influence of X-radiation on activity of lysosomal enzymes (D, L, H cathepsins) in rat spleen tissue and in inoculated rat sarcoma 45 has been investigated. Intact rats and rats with tumors were subjected to whole-body and sarcoma 45 to local irradiation with doses of 0.155 C/kg and 0.31 C/kg in conditions of breathing gas hypoxic mixture containing 90% of nitrogen and 10% of oxygen (GHM-10). The combined exposure to radiation and GHM-10 was shown to produce a certain protective action (e.g. normalized cathepsin activity) in the spleen. In the tumor tissue the protective effect of GHM-10 was absent.     

Soluble and membrane glycoproteins of the rat brain in radiation injury

The content of total protein, glycoproteins, and N-acetylneuraminic acid in different membrane fractions of rat brain tissues was studied 60 min and 24 h following X-irradiation with a dose of 0.31 C/kg. A slight increase in the amount of glycoproteins was noted upon electrophoresis due to the occurrence of low molecular weight carbohydrate-containing proteins. The derangement of the membrane glycoprotein structure is supposed to be one on the causes of radiation destruction of membranes.     

Role of methyl groups in dynamics and evolution of biomolecules

Recent studies have discovered strong differences between the dynamics of nucleic acids (RNA and DNA) and proteins, especially at low hydration and low temperatures. This difference is caused primarily by dynamics of methyl groups that are abundant in proteins, but are absent or very rare in RNA and DNA. In this paper, we present a hypothesis regarding the role of methyl groups as intrinsic plasticizers in proteins and their evolutionary selection to facilitate protein dynamics and activity. We demonstrate the profound effect methyl groups have on protein dynamics relative to nucleic acid dynamics, and note the apparent correlation of methyl group content in protein classes and their need for molecular flexibility. Moreover, we note the fastest methyl groups of some enzymes appear around dynamical centers such as hinges or active sites. Methyl groups are also of tremendous importance from a hydrophobicity/folding/entropy perspective. These significant roles, however, complement our hypothesis rather than preclude the recognition of methyl groups in the dynamics and evolution of biomolecules.     

The effect of synthetic proteinase inhibitors of the level of human recombinant proinsulin production, secreted by a genetically engineered strain of Bacillus subtilis

Influence of O,O,-diethyl-1-(N-alpha-hydrohexafluoroisobutyryl)amino-1- methylpropylphosphonate and O,O-diisobutyl-1-[2-(ethoxycarbonyl)aminoperfluoroprop-2-yl] -1- methylpropylphosphonate on the level of production of human proinsulin secreted by a genetically engineered culture Bacillus subtilis AJ 73 (pBINS1.0.) has been studied. The above phosphonates, being non-toxic for microorganisms, reduced degradation of proinsulin by serine proteinases.     

Isolation of high molecular weight eukaryotic DNA with the use of potassium acetate

The simple technique for isolation of high-molecular eucaryotic DNA has been proposed. It includes cell or nuclei lysates by sodium dodecylsulfate in the presence of pronase, proteins precipitation by potassium acetate, DNA precipitation by ethanol. The DNA isolated by this technique is easily cleaved by restriction endonucleases and can be used for analysis of the unique genes by blot-hybridization. The yield of DNA is similar or somewhat higher than that in case of using the standard methods including phenol extraction or phenol-chloroform extraction.