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排序方式: 共有218条查询结果,搜索用时 15 毫秒
1.
T B Krokhina T G Tsvetkova I V Gar'kavtsev 《Biulleten' eksperimental'no? biologii i meditsiny》1992,113(1):72-74
We have obtained the alphoid DNA clones, pK1 and pK2, from the extrachromosomal DNA of Hela cells treated by cycloheximide (30 micrograms/ml). Nucleotide sequences of the clones were aligned. The sides of the pK1 and pK2 are 390 and 184 bp, respectively. The marked RELP for the clones was not observed. The results of in situ hybridization have shown an approximately equal distribution of Ag-grains over major part of human chromosomes, with a slight preference for chromosomes 1, 5 and 19 (the 1-st group of alpha-satellite DNA). Therefore, the obtained alphoid sequences seem to be rather conservative and non-chromosome-specific. We suppose that increase of the alphoid DNA content in the fraction of the extrachromosomal DNA under the cycloheximide treatment is a result of the sporadic statistical processes rather then consequence of the specific excision. 相似文献
2.
Omar JM Hamza Mecky IN Matee Mainen J Moshi Elison NM Simon Ferdinand Mugusi Frans HM Mikx Wim H van Palenstein Helderman Antonius JMM Rijs André JAM van der Ven Paul E Verweij 《BMC microbiology》2008,8(1):135
Background
In Tanzania, little is known on the species distribution and antifungal susceptibility profiles of yeast isolates from HIV-infected patients with primary and recurrent oropharyngeal candidiasis. 相似文献3.
M. A. Abdurakhmanov I. D. Stolyarov A. G. Il’vesa T. L. Tsvetkova V. V. Lebedev 《Human physiology》2006,32(2):154-156
The relationship between the parameters of the distribution of plantar pressure and the state of a patient with multiple sclerosis has been studied with repeated measurements, before and after (or during) treatment. The possibility of using this method for study of walking function impairment in a chronic demyelinating process is considered. 相似文献
4.
R D Gavriliaka N A Semina L I Fiks S V Shabalina N V Tsvetkova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(1):45-48
ELISA is used for detecting the soluble staphylococcal antigen in patients with purulent septic infections. The optimum conditions for the assay have been established: the dose of staphylococcal gamma globulin for plate sensitization should be 5.0-10.0 micrograms/ml, the pH of the buffer solution 9.6-10.0, the time and temperature of incubation 18-20 hours at 4 degrees C or 5 hours at 37 degrees C. The possibility of using plates manufactured in the USSR has been shown. The sensitivity of the above diagnostic test system is 0.005 microgram/ml. 相似文献
5.
L N Padiukov I V Ershov N V Tsvetkova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(1):40-45
Complex protein preparations obtained from pneumococci have been used for the serological examination of patients with pneumococcal infection. The proposed system of ELISA permits the detection of positive results in about 70% of cases. The specificity of the method is 95%. Special attention is paid to the determination of the criterion indicating the presence of positive results. 相似文献
6.
The work deals with the search for rational schemes of the purification of endonucleases, suitable for the isolation of different enzymes. The scheme using the combination of Blue Sepharose and phosphocellulose has proved to be most universal. The expediency of starting the purification of Haemophilus enzymes on biogel A-0.5m has been established. 相似文献
7.
N P Vaneeva N E Iastrebova N V Tsvetkova I S Khurges L A Bol'shukhina 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(1):33-35
The comparative analysis of the titers of antibodies to the preparations of S. aureus teichoic acids and DNA in the sera of healthy donors and patients with infectious endocarditis and rheumatic carditis was made by means of ELISA. The sera of patients with infectious endocarditis and rheumatic carditis, in contrast to the sera of healthy donors, showed the presence of antibodies to DNA in 23.5-76.2% of cases. The correlation between the presence of antibodies to S. aureus teichoic acids and DNA in the sera of the patients was weakly pronounced. 相似文献
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9.
S. V. Malyshev T. V. Dolmatovich A. V. Voylokov S. P. Sosnikhina N. V. Tsvetkova A. V. Lovtsus N. A. Kartel’ 《Russian Journal of Genetics》2009,45(12):1444-1449
Studies of phenotypical expression of synaptic mutations in combination with the localization of corresponding genes on a
genetic map permit individual stages of the meiotic process to be differentiated. Two rye asynaptic genes, sy1 and sy9, were mapped with the use of microsatellite markers (SSR) in the pericentromeric regions of the long chromosome arms 7R and
2R, respectively. The sy9 gene cosegregated with two SSR markers Xscm43 and Xgwm132. The asynaptic gene sy1 was mapped within the interval between the isozyme locus Aat2 and two cosegregating loci Xrems1188 and Xrems1135 that are located at a distance of 0.4 cM proximally and 0.1 cM distally with respect to the gene lous. Possible evolutionary
relationships of the mapped genes with homeological loci of the Triticeae species and more distant cereal species, such as maize and rice, are discussed. 相似文献
10.