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Aquatic environmental impact associated with stream-crossing by a pipeline was monitored at Archibald Creek, B.C. for two years. Water chemistry and benthic macroinvertebrates were used as monitoring tools. Results indicated that impacts arising from stream-crossing were short-term and non-residual.Funded by a contract from Canadian Arctic Gas Study Limited, calgary, to Aquatic Environments Limited, Calgary.Aquatic Environments Limited, Calgary.Aquatic Environments Limited, Calgary. 相似文献
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Hui Wang Ping‐Kei Chan Si‐Yuan Pan Kwok‐Ho Kwon Yan Ye Jian‐Hong Chu Wang‐Fun Fong Wilson M.S. Tsui Zhi‐Ling Yu 《Journal of cellular biochemistry》2010,110(6):1447-1456
Pathogenesis of nonalcoholic fatty liver disease (NAFLD) is not clear. In this study we aimed to identify proteins involved in NAFLD development in free fatty acids (FFA)‐induced hepatosteatotic cells and in human liver biopsies. Steatosis was induced by incubating a normal human hepatocyte‐derived cell line L‐02 with FFA. Differentially expressed proteins in the steatotic cells were analyzed by two‐dimensional gel electrophoresis‐based proteomics. Involvement of one of the up‐regulated proteins in steatosis was characterized using the RNA interference approach with the steatotic cells. Protein expression levels in liver biopsies of patients with NAFLD were assessed by immunohistochemistry. Proteomic analysis of L‐02 steatotic cells revealed the up‐regulation of ERp57, a condition not previously implicated in NAFLD. Knockdown of ERp57 expression with siRNA significantly reduced fat accumulation in the steatotic cells. ERp57 expression was detected in 16 out of 17 patient biopsies and correlated with inflammation grades or fibrosis stages, while in 5 normal biopsies ERp57 expression was not detectable in hepatocytes. In conclusion, ERp57 was up‐regulated in FFA‐induced steatotic hepatic cells and in NAFLD patient livers and demonstrated steatotic properties in cultured cells. Further investigations are warranted to verify the involvement of ERp57 in NAFLD development. J. Cell. Biochem. 110: 1447–1456, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
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Chromium present in the forms of Cr(VI) or Cr(III) in soils. Since the toxicity and mobility of Cr(VI) are higher than those
of Cr(III), it would be important to estimate soil Cr(VI) accurately in order to assess the phytotoxicity of Cr. Soil redox
potential can influence the distribution of Cr between Cr(VI) and Cr(III) forms, and thus an in situ method which is not affected by the soil redox condition is needed for determining Cr(VI) availability in paddy fields. In
this study, the Cu-saturated selective ion exchange resin (DOWEX M4159), serving as an infinite sink, was embedded in soils
to extract available Cr(VI) from three representative saturated soils with different amounts of Cr(VI). The results suggested
that Cr(VI) reduction occurred in the flooded soils, and the acid environment favored the adsorption and reduction of Cr(VI).
There was a significant dose-response relationship between the soil resin-extractable Cr(VI) and the plant height of rice
seedlings for test soils. The experimental results suggested that the embedded selective ion exchange resin method could be
a suitable in situ method for assessing the phytotoxicity of Cr in flooded soils. 相似文献
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