Photosynthetic properties of leaves of a yellow green mutant of wheat compared to its wild type

We investigated several photosynthetic parameters of a virescent mutant of durum wheat and of its wild-type. Electron transport rate to ferricyanide was the same in the two genotypes when expressed on leaf area basis while O₂ evolution of the leaf tissue in saturating light and CO₂ was slightly higher in the yellow genotype. RuBPCase was also slightly higher. Quantum yield per absorbed light was similar in the two genotypes. P700 and Cyt f were less concentrated in the mutant while PS II was only marginally lower. The light response curve of CO₂ assimilation indicated higher level of photosynthesis of the mutant in high light, which corresponded to a lower non-photochemical quenching compared to the wild-type. It is concluded that the reaction centres, cyt f and chlorophyll are not limiting factors of electron transport in wheat seedlings and that electron transport capacity is in excess with respect to that needed for driving photosynthesis. Since the differences in photosynthesis reflect differences in RuBPCase activity, it is suggested that this enzyme limits photosynthesis in wheat seedlings also at high light intensities.Abbreviations cyt f cytochrome f - chl chlorophyll - PS II photosystem II - Pn_max maximum photosynthesis - RuBCase Ribulose, 1-5,bisphosphate carboxylase     

Influence of training background on jumping height

The aim of this study was to compare the pattern of force production and center of mass kinematics in maximal vertical jump performance between power athletes, recreational bodybuilders, and physically active subjects. Twenty-seven healthy male subjects (age: 24.5 +/- 4.3 years, height: 178.7 +/- 15.2 cm, and weight: 81.9 +/- 12.7 kg) with distinct training backgrounds were divided into 3 groups: power track athletes (PT, n = 10) with international experience, recreational bodybuilders (BB, n = 7) with at least 2 years of training experience, and physically active subjects (PA, n = 10). Subjects performed a 1 repetition maximum (1RM) leg press test and 5 countermovement jumps with no instructions regarding jumping technique. The power-trained group jumped significantly higher (p < 0.05) than the BB and PA groups (0.40 +/- 0.05, 0.31 +/- 0.04, and 0.30 +/- 0.05, respectively). The difference in jumping height was not produced by higher rates of force development (RFD) and shorter center of mass (CM) displacement. Instead, the PT group had greater CM excursion (p < 0.05) than the other groups. The PT and BB groups had a high correlation between jumping height and 1RM test (r = 0.93 and r = 0.89, p < 0.05, respectively). In conclusion, maximum strength seems to be important for jumping height, but RFD does not seem relevant to achieve maximum jumping heights. High RFD jumps should be performed during training only when sport skills have a time constraint for force application.     

Intermittent exercise as a conditioning activity to induce postactivation potentiation

Postactivation potentiation (PAP) is defined as a short-term increase in voluntary muscle activation following a previous conditioning activity (CA). Controversy about PAP is mostly attributed to the characteristics of the CA and the training status of the subjects. While some studies have found that PAP can be induced by series of 5-10 second maximal voluntary isometric contractions or near maximal dynamic contractions (e.g., 3-5 repetition maximum), others have failed to do so. On the other hand, some studies suggest that intermittent contractions can also induce PAP. However, even though PAP was observed, its duration was not taken into account, leaving ground for further investigations. The purpose of this study was threefold: (a) to verify if PAP can progressively enhance performance of voluntary actions throughout a set of intermittent contractions; (b) to verify PAP duration when induced by an intermittent contractions protocol; and (c) to verify if PAP effects were reproducible in different sessions when induced by intermittent contractions. Ten physically active men, not engaged in strength training, underwent 5 randomized experimental sessions, during which they performed a set of 10 unilateral knee extensions (KE) (1 every 30 seconds) at 60 degrees x s(-1) in an isokinetic dynamometer. Peak torque was evaluated over the 10 unilateral KE and at the randomized intervals of 4, 6, 8, 10, and 12 minutes post CA. Peak torque was potentiated 1.3 (+/-0.79) N x m per unilateral KE, and the potentiation effect persisted for 12 minutes after the last contraction. These findings were reproduced in all 5 experimental sessions. Thus, intermittent conditioning activities seem to be an effective way to produce PAP. However, these activities should be tested in a more real world situation to verify the applicability as a warm-up routine.     

The gene for human transforming growth factor alpha is on the short arm of chromosome 2

Transforming growth factor alpha is a polypeptide growth factor that participates in the reversible transformation of cells in vitro and is secreted by many transformed cell lines. It also shares sequence and functional homologies with epidermal growth factor. Working with a cloned cDNA probe (lambda hTGF1-10) and derivatives, we have mapped this gene (TGFA) to 2p13 with the use of somatic cell hybrids and in situ hybridization. This is the same region involved in the 2;8 translocations of Burkitt lymphoma. Such a rearrangement could orient c-myc (8q24) adjacent to TGFA, resulting in activation of one or both of these genes.     

Assignment of the human fibronectin structural gene to chromosome 2

A cloned human cDNA probe for fibronectin (FN) containing 1.3 kb of the human FN coding region has been used to determine the chromosome that encodes the structural gene in human-mouse somatic cell hybrids. The results show that human chromosome 2 encodes the FN structural gene.     

Maximal strength, number of repetitions, and total volume are differently affected by static-, ballistic-, and proprioceptive neuromuscular facilitation stretching

ABSTRACT: Barroso, R, Tricoli, V, dos Santos Gil, S, Ugrinowitsch, C, and Roschel, H. Maximal strength, number of repetitions, and total volume are differently affected by static-, ballistic-, and proprioceptive neuromuscular facilitation stretching. J Strength Cond Res 26(9): 2432-2437, 2012-Stretching exercises have been traditionally incorporated into warm-up routines before training sessions and sport events. However, the effects of stretching on maximal strength and strength endurance performance seem to depend on the type of stretching employed. The objective of this study was to compare the effects of static stretching (SS), ballistic stretching (BS), and proprioceptive neuromuscular facilitation (PNF) stretching on maximal strength, number of repetitions at a submaximal load, and total volume (i.e., number of repetitions × external load) in a multiple-set resistance training bout. Twelve strength-trained men (20.4 ± 4.5 years, 67.9 ± 6.3 kg, 173.3 ± 8.5 cm) volunteered to participate in this study. All of the subjects completed 8 experimental sessions. Four experimental sessions were designed to test maximal strength in the leg press (i.e., 1 repetition maximum [1RM]) after each stretching condition (SS, BS, PNF, or no-stretching [NS]). During the other 4 sessions, the number of repetitions performed at 80% 1RM was assessed after each stretching condition. All of the stretching protocols significantly improved the range of motion in the sit-and-reach test when compared with NS. Further, PNF induced greater changes in the sit-and-reach test than BS did (4.7 ± 1.6, 2.9 ± 1.5, and 1.9 ± 1.4 cm for PNF, SS, and BS, respectively). Leg press 1RM values were decreased only after the PNF condition (5.5%, p < 0.001). All the stretching protocols significantly reduced the number of repetitions (SS: 20.8%, p < 0.001; BS: 17.8%, p = 0.01; PNF: 22.7%, p < 0.001) and total volume (SS: 20.4%, p < 0.001; BS: 17.9%, p = 0.01; PNF: 22.4%, p < 0.001) when compared with NS. The results from this study suggest that, to avoid a decrease in both the number of repetitions and total volume, stretching exercises should not be performed before a resistance training session. Additionally, strength-trained individuals may experience reduced maximal dynamic strength after PNF stretching.     

Comparison of homoserine dehydrogenase from different plant sources

Homoserine dehydrogenase (HSD) was partially purified from castor bean, pea and wheat seedlings. The enzyme from pea had a MW of 75 000 and no sensitivity to threonine when measured in the direction of homoserine formation (forward reaction). The enzyme purified from castor bean had a MW of 290 000–350 000 and exhibited an almost complete inhibition by 1 mM threonine. Furthermore, this enzyme exhibited a polymeric nature as shown by polyacrylamide electrophoresis of the desensitized preparation and by SDS electrophoresis of the native enzyme. In wheat two isoenzymes were separated by gel filtration on Sephadex G 200. The fast-moving fraction (HSD I) was completely inhibited by threonine and exhibited a MW of 280 000, while the slow-moving fraction (HSD II) was insensitive to threonine and had a MW of 75 000. The sensitive enzyme from wheat and castor bean showed an almost absolute requirement for K⁺. The enzyme from pea and the insensitive form from wheat did not show a requirement for K⁺. For the wheat enzyme the effect of several amino acids and the main kinetic constants were studied.     

Development of an Agrobacterium‐delivered CRISPR/Cas9 system for wheat genome editing

CRISPR/Cas9 has been widely used for genome editing in many organisms, including important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the application of this powerful genome editing tool also depends on DNA delivery methods. In wheat, the biolistics based transformation is the most used method for delivery of the CRISPR/Cas9 complex. Due to the high frequency of gene silencing associated with co‐transferred plasmid backbone and low edit rate in wheat, a large T₀ transgenic plant population are required for recovery of desired mutations, which poses a bottleneck for many genome editing projects. Here, we report an Agrobacterium‐delivered CRISPR/Cas9 system in wheat, which includes a wheat codon optimized Cas9 driven by a maize ubiquitin gene promoter and a guide RNA cassette driven by wheat U6 promoters in a single binary vector. Using this CRISPR/Cas9 system, we have developed 68 edit mutants for four grain‐regulatory genes, TaCKX2‐1, TaGLW7, TaGW2, and TaGW8, in T₀, T₁, and T₂ generation plants at an average edit rate of 10% without detecting off‐target mutations in the most Cas9‐active plants. Homozygous mutations can be recovered from a large population in a single generation. Different from most plant species, deletions over 10 bp are the dominant mutation types in wheat. Plants homozygous of 1160‐bp deletion in TaCKX2‐D1 significantly increased grain number per spikelet. In conclusion, our Agrobacterium‐delivered CRISPR/Cas9 system provides an alternative option for wheat genome editing, which requires a small number of transformation events because CRISPR/Cas9 remains active for novel mutations through generations.