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Maria D Mjaavatten Désirée van der Heijde Till Uhlig Anne J Haugen Halvor Nygaard Göran Sidenvall Knut Helgetveit Tore K Kvien 《Arthritis research & therapy》2010,12(3):R76
Introduction
We wanted to assess the importance of the levels of anti-citrullinated peptide antibody (anti-CCP) and immunoglobulin M (IgM) rheumatoid factor (RF) in predicting development of persistent arthritis from undifferentiated arthritis (UA), and to investigate whether there is an added predictive value for persistent arthritis in testing for both anti-CCP and IgM RF. 相似文献3.
Evidence that the peptide HLLVMKGAPER, which can be released from intact sodium and potassium ion activated adenosinetriphosphatase by tryptic digestion, is located on the cytoplasmic surface of the native enzyme has been obtained. An immunoadsorbent directed against the carboxy-terminal sequence of this tryptic peptide has been constructed. The peptide KGAPER was synthesized by solid-phase techniques. Antibodies against the sequence -GAPER were purified by immunoadsorption, using the synthetic peptide attached to agarose beads. These antibodies, in turn, were coupled to agarose beads to produce an immunoadsorbent. Sealed, right-side-out vesicles, prepared from canine kidneys, were labeled with pyridoxal phosphate and sodium [3H]borohydride in the absence or presence of saponin, respectively. A tryptic digest of these labeled vesicles was passed over the immunoadsorbent. Large increases in the incorporation of radioactivity into the peptides bound by the immunoadsorbent were observed in the digests obtained from the vesicles exposed to saponin. From the results of several control experiments examining the labeling reaction as applied to these vesicles, it could be concluded that this increase in incorporation resulted only from the access that the reagents gained to the inside of the vesicles in the presence of saponin and that the increase in the extent of modification was due to the cytoplasmic disposition of this segment in the native enzyme. 相似文献
4.
Topological disposition of the sequences -QRKIVE- and -KETYY in native (Na+ + K+)-ATPase 总被引:1,自引:0,他引:1
R Bayer 《Biochemistry》1990,29(9):2251-2256
The dispositions with respect to the plane of the membrane of lysine-905 in the internal sequence -EQRKIVE- and of lysine-1012 in the carboxy-terminal sequence -RRPGGWVEKETYY of the alpha-polypeptide of sodium and potassium ion activated adenosinetriphosphatase have been determined. These lysines are found in peptides released from the intact alpha-polypeptide by the extracellular protease from Staphylococcus aureus strain V8 and by trypsin, respectively. Synthetic peptides containing terminal sequences of these were used to prepare polyclonal antibodies, which were then used to prepare immunoadsorbents directed against the respective peptides. Sealed, right-side-out membrane vesicles containing native (Na+ + K+)-ATPase were labeled with pyridoxal phosphate and sodium [3H]borohydride in the absence or presence of saponin. The labeled alpha-polypeptide was isolated from these vesicles and digested with appropriate proteases. The incorporation of radioactivity into the peptides binding to the immunoadsorbent directed against the sequence pyrERXIVE increased 3-fold in the presence of saponin as a result of the increased accessibility of this portion of the protein to the reagent when the vesicles were breached by saponin; hence, this sequence is located on the cytoplasmic face of the membrane. It was inferred that the carboxy-terminal sequence -KETYY is on the extracytoplasmic face since the incorporation of radioactivity into peptides binding to the immunoadsorbent directed against the sequence -ETYY did not change when the vesicles were breached with saponin. 相似文献
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The hippocampus and behavioral maturation 总被引:3,自引:0,他引:3
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Raffaele Teperino Sabine Amann Martina Bayer Sean L. McGeeAndrea Loipetzberger Timothy ConnorCarsten Jaeger Bernd KammererLilli Winter Gerhard WicheKevin Dalgaard Madhan SelvarajMichael Gaster Robert S. Lee-YoungMark A. Febbraio Claude KnaufPatrice D. Cani Fritz AbergerJosef M. Penninger J. Andrew Pospisilik Harald Esterbauer 《Cell》2012,151(2):414-426
10.
K. Bayer 《Engineering in Life Science》1997,17(1):81-82