全文获取类型
收费全文 | 1182篇 |
免费 | 105篇 |
国内免费 | 2篇 |
出版年
2022年 | 5篇 |
2021年 | 12篇 |
2020年 | 6篇 |
2019年 | 11篇 |
2018年 | 16篇 |
2017年 | 23篇 |
2016年 | 30篇 |
2015年 | 51篇 |
2014年 | 53篇 |
2013年 | 69篇 |
2012年 | 89篇 |
2011年 | 84篇 |
2010年 | 48篇 |
2009年 | 53篇 |
2008年 | 95篇 |
2007年 | 82篇 |
2006年 | 71篇 |
2005年 | 73篇 |
2004年 | 71篇 |
2003年 | 64篇 |
2002年 | 63篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 7篇 |
1998年 | 6篇 |
1997年 | 9篇 |
1996年 | 6篇 |
1995年 | 11篇 |
1994年 | 17篇 |
1993年 | 8篇 |
1992年 | 14篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 13篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 12篇 |
1985年 | 18篇 |
1984年 | 9篇 |
1983年 | 7篇 |
1982年 | 15篇 |
1981年 | 6篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1975年 | 4篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1966年 | 1篇 |
排序方式: 共有1289条查询结果,搜索用时 15 毫秒
1.
Tatsuya Matsunami Toshihiro Suzuki Yasuo Hisa Kuniaki Takata Tetsuro Takamatsu Masahito Oyamada 《Cell communication & adhesion》2006,13(1):93-102
To elucidate the role of the spiral limbus in glucose transport in the cochlea, we analyzed the expression and localization of GLUT1, connexin26, connexin30, and occludin in the spiral limbus of the rat cochlea. GLUT1 and occludin were detected in blood vessels. GLUT1, connexin26, connexin30, and occludin were also expressed in fibrocytes just basal to the supralimbal lining cells. Connexin26 and connexin30 were present among not only these GLUT1-positive fibrocytes but also GLUT1-negative fibrocytes. In vivo glucose imaging using 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG, MW 342) together with Evans Blue Albumin (EBA, MW 68,000) showed that 6-NBDG was rapidly distributed throughout the spiral limbus, whereas EBA was localized only in the vessels. Moreover, the gap junctional uncoupler heptanol inhibited the distribution of 6-NBDG. These findings suggest that gap junctions play an important role in glucose transport in the spiral limbus, i.e., that gap junctions mediate glucose transport from GLUT1-positive fibrocytes to GLUT1-negative fibrocytes in the spiral limbus. 相似文献
2.
Akira Mine Kiwamu Hyodo Yuri Tajima Kusumawaty Kusumanegara Takako Taniguchi Masanori Kaido Kazuyuki Mise Hisaaki Taniguchi Tetsuro Okuno 《Journal of virology》2012,86(22):12091-12104
Assembly of viral replicase complexes of eukaryotic positive-strand RNA viruses is a regulated process: multiple viral and host components must be assembled on intracellular membranes and ordered into quaternary complexes capable of synthesizing viral RNAs. However, the molecular mechanisms underlying this process are poorly understood. In this study, we used a model virus, Red clover necrotic mosaic virus (RCNMV), whose replicase complex can be detected readily as the 480-kDa functional protein complex. We found that host heat shock proteins Hsp70 and Hsp90 are required for RCNMV RNA replication and that they interact with p27, a virus-encoded component of the 480-kDa replicase complex, on the endoplasmic reticulum membrane. Using a cell-free viral translation/replication system in combination with specific inhibitors of Hsp70 and Hsp90, we found that inhibition of p27-Hsp70 interaction inhibits the formation of the 480-kDa complex but instead induces the accumulation of large complexes that are nonfunctional in viral RNA synthesis. In contrast, inhibition of p27-Hsp90 interaction did not induce such large complexes but rendered p27 incapable of binding to a specific viral RNA element, which is a critical step for the assembly of the 480-kDa replicase complex and viral RNA replication. Together, our results suggest that Hsp70 and Hsp90 regulate different steps in the assembly of the RCNMV replicase complex. 相似文献
3.
Cells of Nitella axilliformis were made tonoplast-free by intracellularperfusion of media containing ethyleneglycol-bis-(ß-aminoethylether)N,N'-tetraaceticacid (EGTA). When the perfusion medium contained ADP as wellas ATP, the membrane hyperpolarized in darkness in a mannersimilar to light-induced hyperpolarization. This light-independenthyperpolarization seems to be due to activation of the electrogenicion pump in the plasma membrane because the hyperpolarized valueof the membrane potential was more negative than the equilibriumpotential for K$, the most negative ion equilibrium potentialin Nitella. The hyperpolarization was inhibited by the respiratory chaininhibitors NaCN (1 mM), antimycin A (10 µM) and rotenone(10 µM). NaCN slightly decreased the ATP concentrationin the cell perfused with medium containing 1 mM ATP and 1 mMADP; but, even after treatment with NaCN, the cell had about80% of the ATP value for the control.
* This study is dedicated to the late Professor J. Ashida. (Received June 24, 1982; Accepted October 15, 1982) 相似文献
4.
Studies on bacteriophage fd DNA. II. Localization of RNA initiation sites on the cleavage map of the fd genome. 总被引:3,自引:0,他引:3
In an in vitro RNA synthesizing system, a single size of A-start RNA and three different sizes of G-start RNA are predominantly transcribed on the doubly closed replicative form (RFI) DNA of phage fd. When the RFI DNA was cleaved into three fragments (HinH-A, HinH-B and HinH-C) by a restriction endonuclease from Haemophilus influenzae H-I, the A-start RNA was predominantly initiated on HinH-B and the three G-start RNAs on HinH-A. RFI DNA was further cleaved into smaller pieces by two other restriction endonucleases from H. aphirophilus and H. gallinarum. Upon mixing the digests with RNA polymerase, two specific fragments derived from HinH-A were bound to the polymerase with GTP present. G-start RNA was efficiently initiated on the fragments isolated by this procedure. On the basis of these observations and estimates of the size of RNA formed on each fragment, the initiation sites for major RNA species were localized on the cleavage map of the phage fd genome previously constructed. 相似文献
5.
6.
7.
8.
Takeshi Hara Tatsunori Kobayashi Satoshi Ito Xiangrong Zhou Tetsuro Katafuchi Hiroshi Fujita 《PloS one》2015,10(5)
Understanding of standardized uptake value (SUV) of 2-deoxy-2-[18F]fluoro-d-glucose positron emission tomography (FDG-PET) depends on the background accumulations of glucose because the SUV often varies the status of patients. The purpose of this study was to develop a new method for quantitative analysis of SUV of FDG-PET scan images. The method included an anatomical standardization and a statistical comparison with normal cases by using Z-score that are often used in SPM or 3D-SSP approach for brain function analysis. Our scheme consisted of two approaches, which included the construction of a normal model and the determination of the SUV scores as Z-score index for measuring the abnormality of an FDG-PET scan image. To construct the normal torso model, all of the normal images were registered into one shape, which indicated the normal range of SUV at all voxels. The image deformation process consisted of a whole body rigid registration of shoulder to bladder region and liver registration and a non-linear registration of body surface by using the thin-plate spline technique. In order to validate usefulness of our method, we segment suspicious regions on FDG-PET images manually, and obtained the Z-scores of the regions based on the corresponding voxels that stores the mean and the standard deviations from the normal model. We collected 243 (143 males and 100 females) normal cases to construct the normal model. We also extracted 432 abnormal spots from 63 abnormal cases (73 cancer lesions) to validate the Z-scores. The Z-scores of 417 out of 432 abnormal spots were higher than 2.0, which statistically indicated the severity of the spots. In conclusions, the Z-scores obtained by our computerized scheme with anatomical standardization of torso region would be useful for visualization and detection of subtle lesions on FDG-PET scan images even when the SUV may not clearly show an abnormality. 相似文献
9.
Shusuke Numata Kazuo Ishii Atsushi Tajima Jun-ichi Iga Makoto Kinoshita Shinya Watanabe Hidehiro Umehara Manabu Fuchikami Satoshi Okada Shuken Boku Akitoyo Hishimoto Shinji Shimodera Issei Imoto Shigeru Morinobu Tetsuro Ohmori 《Epigenetics》2015,10(2):135-141
Aberrant DNA methylation in the blood of patients with major depressive disorder (MDD) has been reported in several previous studies. However, no comprehensive studies using medication-free subjects with MDD have been conducted. Furthermore, the majority of these previous studies has been limited to the analysis of the CpG sites in CpG islands (CGIs) in the gene promoter regions. The main aim of the present study is to identify DNA methylation markers that distinguish patients with MDD from non-psychiatric controls. Genome-wide DNA methylation profiling of peripheral leukocytes was conducted in two set of samples, a discovery set (20 medication-free patients with MDD and 19 controls) and a replication set (12 medication-free patients with MDD and 12 controls), using Infinium HumanMethylation450 BeadChips. Significant diagnostic differences in DNA methylation were observed at 363 CpG sites in the discovery set. All of these loci demonstrated lower DNA methylation in patients with MDD than in the controls, and most of them (85.7%) were located in the CGIs in the gene promoter regions. We were able to distinguish patients with MDD from the control subjects with high accuracy in the discriminant analysis using the top DNA methylation markers. We also validated these selected DNA methylation markers in the replication set. Our results indicate that multiplex DNA methylation markers may be useful for distinguishing patients with MDD from non-psychiatric controls. 相似文献
10.
Tsutomu Murakami Tsutomu Yoshikawa Yuichiro Maekawa Tetsuro Ueda Toshiaki Isogai Konomi Sakata Ken Nagao Takeshi Yamamoto Morimasa Takayama 《PloS one》2015,10(8)