全文获取类型
收费全文 | 881篇 |
免费 | 36篇 |
出版年
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 17篇 |
2014年 | 10篇 |
2013年 | 125篇 |
2012年 | 35篇 |
2011年 | 34篇 |
2010年 | 23篇 |
2009年 | 26篇 |
2008年 | 34篇 |
2007年 | 25篇 |
2006年 | 35篇 |
2005年 | 47篇 |
2004年 | 59篇 |
2003年 | 54篇 |
2002年 | 44篇 |
2001年 | 17篇 |
2000年 | 19篇 |
1999年 | 9篇 |
1998年 | 15篇 |
1997年 | 15篇 |
1996年 | 14篇 |
1995年 | 20篇 |
1994年 | 16篇 |
1993年 | 18篇 |
1992年 | 21篇 |
1991年 | 9篇 |
1990年 | 9篇 |
1989年 | 8篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1982年 | 13篇 |
1981年 | 6篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1976年 | 6篇 |
1975年 | 3篇 |
1974年 | 6篇 |
1973年 | 8篇 |
1972年 | 5篇 |
1970年 | 6篇 |
1960年 | 4篇 |
1959年 | 3篇 |
排序方式: 共有917条查询结果,搜索用时 187 毫秒
1.
Sumiko Hara Chisato Kaneko Hiroatsu Matsumoto Takeshi Nishino Tadao Takeuchi Takeo Mori 《Nucleosides, nucleotides & nucleic acids》2013,32(2-4):571-582
Abstract Novel β-D-ribofuranosides having a 5-substituted imidazo [4,5-d] [1,3]thiazine ring, including the S6-congener 3 of oxanosine 2, were synthesized for screening their anticancer and antiviral activities. 相似文献
2.
Kunisuke Nagamatsu Yasumasa Kido Tadao Terao Takashi Ishida Satoshi Toki 《Life sciences》1982,30(13):1121-1127
The ability of sulfhydryl compounds to provide protection against the acute toxicity of morphinone was investigated in mice. Subcutaneous administration of morphinone produced a reduction of hepatic non-protein sulfhydryl concentration. Pretreatments of mice with glutathione or cysteine significantly increased the survival rate of mice given a lethal dose of morphinone, whereas morphinone lethality was markedly potentiated by diethyl maleate. On the other hand, the administration of morphine produced a dose dependent reduction of hepatic non-protein sulfhydryl contents. However, neither glutathione nor cysteine protected mice from the acute toxicity of morphine. A possible explanation for these observations was proposed as follows: morphine is oxidized by morphine 6-dehydrogenase to morphinone, and the morphinone thus produced decreases the sulfhydryl contents in the liver. This mechanism is supported by the fact that morphinone reacts easily with glutathione and cysteine . 相似文献
3.
4.
5.
Z. Kato Seiji Fukuda Shunji Tomatsu Hugo Vega Teruo Yasunaga Atsushi Yamagishi Naoto Yamada A. Valencia Luis Alejandro Barrera Kazuko Sukegawa Tadao Orii Naomi Kondo 《Human genetics》1997,101(1):97-101
Mucopolysaccharidosis IVA (MPS IVA) is an autosomal recessive lysosomal storage disorder caused by a genetic defect in N-acetylgalactosamine-6-sulfate
sulfatase (GALNS). In previous studies, we have found two common mutations in Caucasians and Japanese, respectively. To characterize
the mutational spectrum in various ethnic groups, mutations in the GALNS gene in Colombian MPS IVA patients were investigated,
and genetic backgrounds were extensively analyzed to identify racial origin, based on mitochondrial DNA (mtDNA) lineages.
Three novel missense mutations never identified previously in other populations and found in 16 out of 19 Colombian MPS IVA
unrelated alleles account for 84.2% of the alleles in this study. The G301C and S162F mutations account for 68.4% and 10.5%
of mutations, respectively, whereas the remaining F69V is limited to a single allele. The skewed prevalence of G301C in only
Colombian patients and haplotype analysis by restriction fragment length polymorphisms in the GALNS gene suggest that G301C
originated from a common ancestor. Investigation of the genetic background by means of mtDNA lineages indicate that all our
patients are probably of native American descent.
Received: 2 January 1997 / Accepted: 10 June 1997 相似文献
6.
Tadao Kunihiro Bart Veuger Diana Vasquez-Cardenas Lara Pozzato Marie Le Guitton Kazuyoshi Moriya Michinobu Kuwae Koji Omori Henricus T. S. Boschker Dick van Oevelen 《PloS one》2014,9(4)
Phospholipid-derived fatty acids (PLFA) and respiratory quinones (RQ) are microbial compounds that have been utilized as biomarkers to quantify bacterial biomass and to characterize microbial community structure in sediments, waters, and soils. While PLFAs have been widely used as quantitative bacterial biomarkers in marine sediments, applications of quinone analysis in marine sediments are very limited. In this study, we investigated the relation between both groups of bacterial biomarkers in a broad range of marine sediments from the intertidal zone to the deep sea. We found a good log-log correlation between concentrations of bacterial PLFA and RQ over several orders of magnitude. This relationship is probably due to metabolic variation in quinone concentrations in bacterial cells in different environments, whereas PLFA concentrations are relatively stable under different conditions. We also found a good agreement in the community structure classifications based on the bacterial PLFAs and RQs. These results strengthen the application of both compounds as quantitative bacterial biomarkers. Moreover, the bacterial PLFA- and RQ profiles revealed a comparable dissimilarity pattern of the sampled sediments, but with a higher level of dissimilarity for the RQs. This means that the quinone method has a higher resolution for resolving differences in bacterial community composition. Combining PLFA and quinone analysis as a complementary method is a good strategy to yield higher resolving power in bacterial community structure. 相似文献
7.
8.
9.
Tadashi Ogawa Takahiko Hlgasa Tadao Hata 《Bioscience, biotechnology, and biochemistry》2013,77(5):712-723
The proteinase inhibitors I (R-I) and III (R-III) isolated from Japanese radish seed were characterized in terms of their N-terminal amino acids, amino acid composition and reacting groups. The amino acid composition of two proteins differed from each other, while histidine, methionine and tryptophan contents were all low. N-Terminal amino acids of these inhibitors determined by Edman degradation were the same; valine.By modifying free amino groups in the inhibitors with trinitrobenzenesulfonic acid, R-III was greatly inactivated in proportion to the modification of amino groups, but the activity of R-I was not affected.However, modification of arginyl residues of R-I by cyclohexanedione reduced its activity. These results indicate that R-I is an arginine-type and R-III is a lysine-type inhibitor. 相似文献
10.
Takayoshi Yamagishi Toshihiko Serikawa Ryoko Morita Shinichi Nakamura Shoki Nishida 《Microbiology and immunology》1976,20(5):397-403
TSN agar was applicable for enumeration of Clostridium perfringens in fecal samples of adults but not in those of infants. It was demonstrated using TSN agar that some healthy aged adults had persistently carried C. perfringens at levels ranging from 107 to 109, while some others ranged from 103 to 106 per ml volume of fecal sample although all of these adults had the same diets. In the test for agglutinability of isolates of C. perfringens collected from two elderly adults, a younger adult and a baby, it was demonstrated that most of the isolates obtained from an aged adult of high levels for 19 months belonged the same serotype, while rapid alteration of serotypes could be observed in three other persons with high or low levels. In spite of as many as 109 C. perfringens per ml of feces, no trace of α-toxin could be detected in the fecal samples. In in vitro tests, fecal suspension suppressed the production of α-toxin although it allowed the organism to grow sufficiently. 相似文献