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1.
Richard Campen Julia Kowalski W. Berry Lyons Slawek Tulaczyk Bernd Dachwald Erin Pettit Kathleen A. Welch Jill A. Mikucki 《Environmental microbiology》2019,21(7):2290-2306
Antarctic subglacial environments host microbial ecosystems and are proving to be geochemically and biologically diverse. The Taylor Glacier, Antarctica, periodically expels iron-rich brine through a conduit sourced from a deep subglacial aquifer, creating a dramatic red surface feature known as Blood Falls. We used Illumina MiSeq sequencing to describe the core microbiome of this subglacial brine and identified previously undetected but abundant groups including the candidate bacterial phylum Atribacteria and archaeal phylum Pacearchaeota. Our work represents the first microbial characterization of samples collected from within a glacier using a melt probe, and the only Antarctic subglacial aquatic environment that, to date, has been sampled twice. A comparative analysis showed the brine community to be stable at the operational taxonomic unit level of 99% identity over a decade. Higher resolution sequencing enabled deconvolution of the microbiome of subglacial brine from mixtures of materials collected at the glacier surface. Diversity patterns between this brine and samples from the surrounding landscape provide insight into the hydrological connectivity of subglacial fluids to the surface polar desert environment. Understanding subice brines collected on the surfaces of thick ice covers has implications for analyses of expelled materials that may be sampled on icy extraterrestrial worlds. 相似文献
2.
P element-mediated transformation has been usedto investigate the regulation of expression of thesn-glycerol-3-phosphate dehydrogenase gene ofDrosophila melanogaster. A 13-kb constructcontaining the eight exons and associated introns, 5 kb of the5′ region, and 3 kb downstream from the structuralgene produced normal levels of enzyme activity andrescued the poor viability of flies lacking the enzyme. All the regulatory elements essential fornormal enzyme expression were located in a fragment thatincluded the exons and introns and 1-kb upstreamnoncoding sequence. Deletions of the 1.6-kb secondintron reduced activity to 25%. Transformants withfusion constructs between the sn-glycerol-3-phosphatedehydrogenase gene and the beta-galactosidase gene fromE. coli revealed three elements that affectedexpression. A (CT)9 repeat element at the5′ end of the second intron increased expressionin both larvae and adults, particularly at emergence. Asecond regulatory element, which includes a(CT)7 repeat, was located 5′ to the TATA box and had similareffects on the gene's expression. A third, undefined,enhancer was located in the second intron, between 0.5and 1.8 kb downstream of the translation initiationcodon. This element increases enzyme activity to asimilar extent in larvae and adults but has littleeffect when the enhancer at the 5′ end of theintron is present. 相似文献
3.
The distribution and diversity of copepods of the genus Calanus were investigated in Hornsund Fjord (on the southwest coast of Spitsbergen) in summer 2001. The Bhattacharya method was used
to sort individuals by species based on their prosome length. The established prosome length boundary values for the Calanus copepodid stages coincided with those defined for the Calanus species from Kongsfjorden (on the northwest coast of Spitsbergen). The predominant species in the main and inner fjord basins
was Calanus glacialis, whereas Calanus finmarchicus was the prevailing species outside Hornsund. Younger copepodid stages (CI–CIII) of both species concentrated in the surface
water layers (0–50∼70 m), while older copepodids (CIV–CVI females) that were ready for wintering stayed in deep layers (50∼70 m
to bottom). Calanus hyperboreus was present in low numbers, predominantly as CIV, and in Hornsund deep water layers. The distribution and diversity of Calanus species complied with the notion that the marine fauna in Hornsund is of a more Arctic character than in Kongsfjorden, a
fjord 260 km to the north on the west coast of Spitsbergen. 相似文献
4.
The west coast of Spitsbergen is influenced by water masses of Atlantic and Arctic origin. During the winter of January–April
2006, water temperatures on the West Spitsbergen Shelf were ∼3°C warmer than typical winter conditions, leading to a coastal
sea ice cover of reduced thickness, extent and duration. A sediment trap deployed from September 2005 to May 2006 in the outer
basin of Kongsfjorden (NW Spitsbergen) at a depth of 115 m has provided a continuous winter time-series of zooplankton during
a period of rapid increase in water temperatures. Prior to an anomalous and prolonged influx of warm Atlantic water (AW) starting
at the end of January, the trap samples were dominated by the boreo-Arctic copepod Metridia longa. Species that increased in abundance during the influx included late stages of Calanus finmarchicus, C. glacialis, C. hyperboreus and Paraeuchaeta norvegica. The early introduction of shelf populations into the fjord, and thus increased copepod biomass relative to typical winter
conditions with little advection, has implications for the marine pelagic food web and pelagic-benthic coupling. 相似文献
5.
6.
Blachowiak-Samolyk Katarzyna; Kwasniewski Slawek; Hop Haakon; Falk-Petersen Stig 《Journal of plankton research》2008,30(3):311-323
Zooplankton was studied on eight stations in the marginal icezone (MIZ) of the Barents Sea, in May 1999, along two transectsacross the ice edge. On each station, physical background measurementsand zooplankton samples were taken every 6 h over a 24 h periodat five discrete depth intervals. Cluster analysis revealedseparation of open water stations from all ice stations as wellas high similarity level among replicates belonging to particularstation. Based on five replicates per station, analysis of variance(ANOVA) confirmed significant differences (P < 0.05) in abundancesof the main mesozooplankton taxa among stations. Relations betweenthe zooplankton community and environmental parameters wereestablished using redundancy analysis (CANOCO). In total, 55%of mesozooplankton variability within studied area was explainedby eight variables with significant conditional effects: depthstratum, fluorescence, temperature, salinity, bottom depth,latitude, bloom situation, and ice concentration. GLM modelssupported supposition about clear and negative relationshipbetween concentration of Oithona similis, and overall mesozooplanktondiversity. The analyses showed a dynamic relationship betweenmesozooplankton distribution and hydrological conditions onshort-term scale. Furthermore, our study demonstrated that variabilityin the physical environment of dynamic MIZ of the Barents Seahas measurable effect on the Arctic pelagic ecosystem. 相似文献
7.
Body size is a key determinant of fitness in Daphnia. Bigger size means higher feeding efficiency and reproduction. However, filamentous cyanobacteria have a more detrimental effect on larger daphnids. Predicted global warming is expected to reduce size of Daphnia and facilitate frequent occurrence of cyanobacteria. Therefore, we asked two questions: (i) does elevated temperature cause reduced size of daphnids, and (ii) is temperature-dependent size reduction adaptive in an environment dominated by filamentous cyanobacteria? To address these issues, we obtained 8 clones of the Daphnia longispina complex from artificially heated lakes and 8 clones from control lakes, and exposed them to a favorable food Scenedesmus obliquus and a mixture of S. obliquus and the filamentous cyanobacterium Cylindrospermopsis raciborskii in life table experiments at 16, 20, and 24°C. Individuals from heated lakes attained larger body size than those from control lakes. Moreover, exposure to the filamentous cyanobacterium led to reduced fecundity of Daphnia from the non-heated lakes and did not affect reproduction of Daphnia from the heated lakes. We conclude that Daphnia display some evolutionary adaptations to cope with filamentous cyanobacteria, linked to long-term exposition to elevated temperature. Our results violate broadly accepted assumptions that ectotherms are smaller in warmer environments. 相似文献
8.
Groundwater is a major influence on the hydrological, chemical and thermal regime of chalk streams in the southern U.K. However, little is currently known about the nature of the sediment delivery system within these chalk stream systems, even though sediment-related problems have been increasingly cited as a cause of habitat degradation and of declining salmonid stocks. To address this knowledge gap, suspended sediment fluxes were monitored at 4 sites within the Hampshire Avon catchment between February 1999 and August 2000. Maximum suspended sediment concentrations ranged from nearly 45 mg l–1 to 260 mg l–1. Over the study period, annual suspended sediment loads ranged from 644 to 6215 t yr–1 and annual specific sediment yields ranged from 1.4 to 12.5 t km–2 yr–1. The results show that, relative to other U.K. rivers, the study chalk streams are characterised by low suspended sediment concentrations and loads and less episodic behaviour. 相似文献
9.
Submerged macrophytes are known to serve as refuge for zooplankton but also seem to suppress the zooplankton growth. Thus,
there is a conflict between the positive and negative role of macrophytes for zooplankton. We tested the influence of physical
structure using artificial macrophytes, chemicals released by a macrophyte (Myriophyllum verticillatum), as well as the combined effects of these two factors on the life history of Daphnia magna. Daphnids matured at a smaller size and produced fewer eggs but larger individual offspring in the presence of artificial
and real plants. Thus, under conditions with plants, we observed a trade off between number of eggs produced and the individual
size of the offspring. Daphnids grown in the presence of exudates without plants were larger at maturity and showed no reduction
in clutch size as compared with the control. We suggest that the macrophytes (real and artificial) negatively affected the
daphnids in two ways: (1) food particles were settling down faster on the plant structures and this reduced the available
food for Daphnia, (2) the plant structures were obstacles for the daphnids causing them to spent more energy during swimming. Both effects
resulted in a reduced somatic growth, whereas the increased individual offspring size was probably a response to the reduced
food level. Daphnia was not negatively affected by Myriophyllum exudates. 相似文献
10.
S Wray M Self;NINDS Parkinson's Disease iPSC Consortium;NINDS Huntington's Disease iPSC Consortium;NINDS ALS iPSC Consortium PA Lewis JW Taanman NS Ryan CJ Mahoney Y Liang MJ Devine UM Sheerin H Houlden HR Morris D Healy JF Marti-Masso E Preza S Barker M Sutherland RA Corriveau M D'Andrea AH Schapira RJ Uitti M Guttman G Opala B Jasinska-Myga A Puschmann C Nilsson AJ Espay J Slawek L Gutmann BF Boeve K Boylan AJ Stoessl OA Ross NJ Maragakis J Van Gerpen M Gerstenhaber K Gwinn TM Dawson 《PloS one》2012,7(8):e43099
Our understanding of the molecular mechanisms of many neurological disorders has been greatly enhanced by the discovery of mutations in genes linked to familial forms of these diseases. These have facilitated the generation of cell and animal models that can be used to understand the underlying molecular pathology. Recently, there has been a surge of interest in the use of patient-derived cells, due to the development of induced pluripotent stem cells and their subsequent differentiation into neurons and glia. Access to patient cell lines carrying the relevant mutations is a limiting factor for many centres wishing to pursue this research. We have therefore generated an open-access collection of fibroblast lines from patients carrying mutations linked to neurological disease. These cell lines have been deposited in the National Institute for Neurological Disorders and Stroke (NINDS) Repository at the Coriell Institute for Medical Research and can be requested by any research group for use in in vitro disease modelling. There are currently 71 mutation-defined cell lines available for request from a wide range of neurological disorders and this collection will be continually expanded. This represents a significant resource that will advance the use of patient cells as disease models by the scientific community. 相似文献