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排序方式: 共有183条查询结果,搜索用时 15 毫秒
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N Tanaka H Nakada N Itoh Y Mizuno M Takanishi T Kawasaki S Tate F Inagaki I Yamashina 《Journal of biochemistry》1992,112(1):68-74
The structure of the Asn-linked carbohydrate chain of batroxobin, a thrombin-like enzyme from Bothrops atrox moojeni snake venom, has been determined. The sugar chain was isolated from batroxobin by hydrazinolysis followed by pyridylamination (PA). The PA-oligosaccharide chain was purified by HPLC on an anion exchange or reverse phase columns, and its structure was examined by sequential exoglycosidase digestion, 600 MHZ 1H NMR spectroscopy and methylation analysis. The results indicate that the oligosaccharide chain has the following structure involving a novel linkage, NeuAc alpha 2----3GalNAc. 相似文献
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Structural studies on phosphorylated oligosaccharides derived from yeast mannan by 1H(31P) relayed spin-echo difference spectroscopy (RESED) 总被引:1,自引:0,他引:1
P de Waard J F Vliegenthart Y Kozutsumi T Kawasaki I Yamashina 《The Journal of biological chemistry》1989,264(21):12141-12144
Phosphorylated and nonphosphorylated tri- and tetrasaccharides were isolated from yeast mannan and analyzed by 500-MHz 1H NMR spectroscopy. Relayed spin-echo difference spectroscopy (RESED) was used to reveal subspectra of the phosphorylated residues. In this way the attachment position of the phosphate group could be established. The structures of the phosphorylated compounds turned out to be (Formula: see text) and (Formula: see text) RESED spectroscopy has proved to be a suitable method in analyzing low amounts of phosphorylated oligosaccharides. 相似文献
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The structures of two glycoasparagines composed of one mole each of N-acetylneuraminic acid, galactose, N-acetylglucosamine, and asparagine were determined by periodate oxidation, enzymatic degradation, and methylation analysis. The structures were NANAalpha2 leads to 3Galbeta1 leads to 4GlcNAcbeta leads to Asn and NANAalpha2 leads to 6Galbeta1 leads to 4GlcNAcbeta leads to Asn, respectively. 相似文献
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Enzymatic cleavage of glycopeptides 总被引:14,自引:0,他引:14
M Makino T Kojima I Yamashina 《Biochemical and biophysical research communications》1966,24(6):961-966
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