全文获取类型
收费全文 | 458篇 |
免费 | 34篇 |
国内免费 | 1篇 |
出版年
2022年 | 2篇 |
2021年 | 5篇 |
2019年 | 3篇 |
2018年 | 7篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 11篇 |
2014年 | 14篇 |
2013年 | 16篇 |
2012年 | 19篇 |
2011年 | 30篇 |
2010年 | 13篇 |
2009年 | 13篇 |
2008年 | 11篇 |
2007年 | 16篇 |
2006年 | 28篇 |
2005年 | 28篇 |
2004年 | 26篇 |
2003年 | 35篇 |
2002年 | 14篇 |
2001年 | 11篇 |
2000年 | 14篇 |
1999年 | 20篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 6篇 |
1991年 | 9篇 |
1990年 | 11篇 |
1989年 | 7篇 |
1988年 | 11篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1975年 | 5篇 |
1974年 | 6篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1961年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有493条查询结果,搜索用时 16 毫秒
1.
Summary The sequence of the synthesis and transport of the organic matrix of spicules has been elucidated in the gorgonian Leptogorgia virgulata by use of 3H-aspartic acid as the tracer in electron-microscopic autoradiography. The entire process of matrix synthesis and transport takes approximately 2 h. It seems that the protein moiety of the organic matrix is synthesized in the RER prior to 5 min following the initial 10 min incubation in the tracer. At the 5 min chase the label is moving from the RER to the Golgi complexes where the carbohydrate moiety of the matrix is presumed to be synthesized. At the 5 to 15 min chases the label is transported out of the Golgi complexes via Golgi vesicles. This phase continues for 30 min. From 60 to 120 min the 3H-aspartic acid moves to the spicules. After 120 min the majority of the label has moved into the spicules. Silver grain counts over both multivesicular and electron-dense bodies remain at relatively low and constant levels over 4 h indicating that neither organelle is involved in the synthesis and transport of the organic matrix.Contribution No 512; Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina, Columbia, South Carolina 29208, USA 相似文献
2.
3.
4.
5.
6.
Tadashi Watabe Tadashi Sawahata 《Biochemical and biophysical research communications》1978,83(4):1396-1403
Recently proposed 20,22-epoxycholesterol as the obligatory intermediate in the NADPH-dependent conversion of cholesterol to pregnenolone by bovine adrenal cortex mitochondria was isolated and found to be a misassigned metabolite. It was identified as 5α,6α-epoxycholestan-3β-ol by gas-liquid chromatography-mass spectrometry as well as by the reverse isotope dilution method. The thin-layer chromatographic behavior of the proposed 20,22-epoxide which had been reported to have a higher polarity than pregnenolone was in good accordance with that of the identified 5α,6α-epoxycholestan-3β-ol. All four diastereoisomers of 20,22-epoxycholesterol had much lower polarity than pregnenolone in thin-layer chromatograms obtained in various solvent systems. Endogeneous cholesterol, contained originally in the mitochondria, was also converted in the presence of NADPH to pregnenolone and 5α,6α-epoxycholestan-3β-ol as observed with radioactive cholesterol added as the exogeneous substrate. 相似文献
7.
S Watabe D J Hartshorne 《Comparative biochemistry and physiology. B, Comparative biochemistry》1990,96(4):639-646
1. Catch is a mechanism found in many molluscan smooth muscles in which tension is maintained at relatively low energy cost. 2. Paramyosin forms the core of thick filaments. In catch muscle paramyosin concentrations are high and the thick filaments are relatively long. 3. The mechanism of catch is not understood, but the consensus is that tension during catch is borne by slowly-cycling cross-bridge attachments to actin. 4. Stimulation by acetylcholine increases intracellular Ca2+ and initiates a contraction characterized by a relatively rapid cross-bridge cycling. Reduction of Ca2+ can lead to relaxation or catch. Relaxation occurs only when a second neurotransmitter, serotonin, is present. 5. The catch state is released by serotonin, via activation of adenylate cyclase, increased levels of cAMP and phosphorylation of one or more contractile proteins, possibly paramyosin. Other targets for phosphorylation are discussed. 6. The contractile cycle of catch muscles, therefore, is controlled by both Ca2+ and cAMP. 相似文献
8.
Hasan Sharmin Asakawa Shuichi Watabe Shugo Kinoshita Shigeharu 《Marine biotechnology (New York, N.Y.)》2021,23(5):821-835
Marine Biotechnology - The human sarcomeric myosin heavy chain gene MYH14 contains an intronic microRNA, miR-499. Our previous studies demonstrated divergent genomic organization and expression... 相似文献
9.
Kijima Yusuke Wantong Wang Igarashi Yoji Yoshitake Kazutoshi Asakawa Shuichi Suzuki Yutaka Watabe Shugo Kinoshita Shigeharu 《Marine biotechnology (New York, N.Y.)》2022,24(5):895-910
Marine Biotechnology - Most mammals, including humans, show obvious aging phenotypes, for example, loss of tissue plasticity and sarcopenia. In this regard, fish can be attractive models to study... 相似文献
10.