全文获取类型
收费全文 | 2898篇 |
免费 | 189篇 |
国内免费 | 1篇 |
出版年
2023年 | 5篇 |
2022年 | 8篇 |
2021年 | 30篇 |
2020年 | 14篇 |
2019年 | 37篇 |
2018年 | 52篇 |
2017年 | 37篇 |
2016年 | 55篇 |
2015年 | 90篇 |
2014年 | 123篇 |
2013年 | 234篇 |
2012年 | 175篇 |
2011年 | 209篇 |
2010年 | 123篇 |
2009年 | 124篇 |
2008年 | 207篇 |
2007年 | 200篇 |
2006年 | 197篇 |
2005年 | 169篇 |
2004年 | 179篇 |
2003年 | 167篇 |
2002年 | 163篇 |
2001年 | 23篇 |
2000年 | 31篇 |
1999年 | 43篇 |
1998年 | 40篇 |
1997年 | 31篇 |
1996年 | 15篇 |
1995年 | 20篇 |
1994年 | 25篇 |
1993年 | 20篇 |
1992年 | 29篇 |
1991年 | 19篇 |
1990年 | 21篇 |
1989年 | 21篇 |
1988年 | 20篇 |
1987年 | 14篇 |
1986年 | 8篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1982年 | 9篇 |
1981年 | 10篇 |
1980年 | 12篇 |
1979年 | 5篇 |
1976年 | 10篇 |
1975年 | 4篇 |
1974年 | 7篇 |
1972年 | 5篇 |
1971年 | 4篇 |
排序方式: 共有3088条查询结果,搜索用时 46 毫秒
1.
Kenji Tsuji Shinji KitamuraHirofumi Makino 《Biochemical and biophysical research communications》2014
The kidneys are exposed to hypoxic conditions during development. Hypoxia-inducible factor (HIF), an important mediator of the response to hypoxia, is believed to have an important role in development. However, the relationship between HIF and branching morphogenesis has not been elucidated clearly. 相似文献
2.
3.
Masaru Manabe Tetsuhisa Goto Shinji Matsuura 《Bioscience, biotechnology, and biochemistry》2013,77(11):2003-2007
Aflatoxins Bl, B2, G1 and G2 were quantitatively detected by high-performance liquid chromatography on a 12 µl flow-cell in the fluorometric detector using the mobile phase of toluene system instead of chloroform, dichloromethane or methanol system. Various kinds of columns and mobile phases were tested, and fine mutual separation of all the four aflatoxins without quenching their fluorescence was achieved by using sHica gel column and toluene- ethyl acetate-formic acid-methanol (89.0: 7.5: 2.0: 1.5 v/v/v/v). The relationship between the fluorescence peak area and the amount injected was linear in the range of 0.3 ng to 120 ng. This method, as applied to food and feed extracts, is sensitive at the 10~20 ppb levels of the four kinds of aflatoxins. 相似文献
4.
Immunological memory for T and B cells was studied in an in vitro culture system with spleen cells from mice primed with bovine serum albumin (BSA). Spleen cells taken from mice immunized at various times previously with a single intravenous injection of alum-precipitated (AP) BSA and bacterial endotoxin (ET) were cultured in Marbrook's system with dinitrophenylated (DNP) BSA as the in vitro antigen. In the cultures of spleen cells obtained from mice primed more than 14 days previously, an IgG-predominant anti-BSA response was generated. However, no anti-BSA response was observed in the culture of spleen cells taken from mice primed 7 days previously (day 7 spleen cells). The failure of day 7 spleen cells to generate an antibody response in vitro was shown to be attributable to both the lack of B memory cells and the effect of “suppressive” macrophages induced by ET. On the other hand, anti-BSA memory in the spleen of mice primed with AP-BSA plus ET and 2 months later challenged with AP-BSA matured within 7 days and declined rather quickly by 30 days after the challenge. The difference in the time course of the generation of memory between the spleen cells from primary and from secondary immunized mice might be attributable to the difference in the maturation of memory B cells, since the time course of the development of memory T cells after the secondary immunization was similar to that observed after primary immunization. 相似文献
5.
Hideaki Naoe Tatsuyuki Chiyoda Jo Ishizawa Kenta Masuda Hideyuki Saya Shinji Kuninaka 《Biochemical and biophysical research communications》2013,430(2):757-762
Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation. 相似文献
6.
Formation of 3',5'-O-(dialkylsilanediyl)deoxyribonucleosides was studied. Treatment of deoxythymidine in DMF with bifunctional silylating reagent such as di-t-butyldichlorosilane and diisopropyldichlorosilane in the presence of imidazole gave the expected silanediyl derivatives. The structure was confirmed mainly by NMR spectroscopy. The stability of these cyclic silyl derivatives toward hydrolysis is also described. 相似文献
7.
8.
Misato Suzuki Fumiya Nakamura Emi Taguchi Maho Nakata Fumi Wada Momoka Takihi Tomoyo Inoue Shinji Ohta Hiroyuki Kawachi 《Molecular and cellular biochemistry》2018,438(1-2):35-45
Human calcium/calmodulin-dependent protein kinase IV (CAMKIV) is a member of Ser/Thr kinase family, and is associated with different types of cancer and neurodegenerative diseases. Vanillin is a natural compound, a primary component of the extract of the vanilla bean which possesses varieties of pharmacological features including anti-oxidant, anti-inflammatory, anti-bacterial and anti-tumor. Here, we have investigated the binding mechanism and affinity of vanillin to the CAMKIV which is being considered as a potential drug target for cancer and neurodegenerative diseases. We found that vanillin binds strongly to the active site cavity of CAMKIV and stabilized by a large number of non-covalent interactions. We explored the utility of vanillin as anti-cancer agent and found that it inhibits the proliferation of human hepatocyte carcinoma (HepG2) and neuroblastoma (SH-SY5Y) cells in a dose-dependent manner. Furthermore, vanillin treatment resulted into the significant reduction in the mitochondrial membrane depolarization and ROS production that eventually leads to apoptosis in HepG2 and SH-SY5Y cancer cells. These findings may offer a novel therapeutic approach by targeting the CAMKIV using natural product and its derivative with a minimal side effect. 相似文献
9.
10.
Katsura Kogure Noriyoshi Sueda Kunio Nakagawa Hideaki Fukawa 《Bioscience, biotechnology, and biochemistry》2013,77(2):435-436
The relationships between dietary levels of the essential amino acids and hepatic polysome profiles of rats were investigated with special attention to the amino acid requirement pattern for the maximum rat growth as determined by other investigators. The basal diet contained a 7% essential amino acid mixture and a 3% non-essential amino acid mixture, with appropriate amounts of other nutrients. Rats were fed test diet for 5 hours and then the polysome profile was determined. The amounts of essential amino acids needed for maximum aggregation of polysome were low for methionine-cystine, leucine and tryptophan as compared with requirements for maximum growth. But in other essential amino acids, the amounts were in almost the same range as those reported for maximum growth by others. The differences between the amino acid requirement patterns for maximum aggregation of hepatic ribosomes and for maximum growth of rats might be due to a difference in amino acid requirements of the liver and whole body. Therefore, the hepatic polysome profile might be used to measure the effect of amino acid supplementation on dietary proteins. The requirement pattern of essential amino acids in other organs may be studied by polysome profile determination. 相似文献