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C Balbi R Bordone A Gelardi F Marchesini M Piccardo D S Sarma M Taningher S Parodi 《Cancer biochemistry biophysics》1990,11(2):135-144
One percent orotic acid supplemented diet is a promoting treatment in the rat model of liver carcinogenesis. After treatment with this type of diet, DNA alterations were observed using alkaline sucrose gradients and alkaline elution methods. In this work we have utilized two unwinding methods for the detection of DNA fragmentation. One method is a viscosimetric method in which the rate of increase in DNA viscosity with time is related to the rate of alkaline DNA unwinding. The second method measures fluorimetrically the amount of renatured and denatured DNA after different times allowed for alkaline DNA unwinding. These two methods are very sensitive in detecting DNA breaks induced by typical alkylating agents, X-rays and H2O2. The two unwinding methods were clearly negative for the orotic acid supplemented diet. We suggest that the DNA alterations detected with alkaline sucrose gradients and alkaline elution methods, after promoting treatment with orotic acid, are probably different from the DNA breaks induced by typical alkylating agents, X-rays and H2O2. 相似文献
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A survey of cats and humans for prevalence of feline leukemia-sarcoma virus neutralizing serum antibodies 总被引:4,自引:0,他引:4
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Ankit Verma Dhirendra Kumar Sharma Rituparna Sarma Hasnahana Chetia Juri Saikia 《Bioinformation》2013,9(15):755-758
BDNF (Brain derived neurotrophic factor) is a secretion protein and a member of the neurotrophin family of growth factors.
Structural and functional characterization of BDNF Varanus komodoensis is of interest while its structure remains unknown. Thus, a
homology molecular model of BDNF was constructed for gleaning possible structural insights. The model was compared with the
structure of the homologous NGF (Nerve growth factor, another member of neuro-trophin family) from Naja atra. Comparative
structural analysis of the models showed structural similarities with their predicted cavities for the interpretation of potential
functional analogy. 相似文献
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