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Irisappan Ganesh Sambandam Ravikumar Ik-keun Yoo Soon Ho Hong 《Bioprocess and biosystems engineering》2015,38(4):797-804
In an attempt to develop a high-throughput screening system for screening microorganisms which produce high amounts of malate, a MalKZ chimeric HK-based biosensor was constructed. Considering the sequence similarity among Escherichia coli (E. coli) MalK with Bacillus subtilis MalK and E. coli DcuS, the putative sensor domain of MalK was fused with the catalytic domain of EnvZ. The chimeric MalK/EnvZ TCS induced the ompC promoter through the cognate response regulator, OmpR, in response to extracellular malate. Real-time quantitative PCR and GFP fluorescence studies showed increased ompC gene expression and GFP fluorescence as malate concentration increased. By using this strategy, various chimeric TCS-based bacteria biosensors can be constructed, which may be used for the development of biochemical-producing recombinant microorganisms. 相似文献
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Interaction of four luminescent rhenium(I) diimine complexes, [Re(CO)3(N-N)L]PF6 ((N-N = 2,2-bipyridine, L = py-3-COOH) 1a, (N-N = 2,2-bipyridine, L = py-3-CONH2) 1b, (N-N = 1,10-phenanthroline, L = py-3-COOH) 2a, (N-N = 1,10-phenanthroline, L = py-3-CONH2) 2b with bovine serum albumin (BSA) at physiological pH has been examined using UV-Vis absorption and luminescence spectroscopy, excited state lifetime measurement and circular dichroism (CD). In the presence of BSA, the luminescence of Re(I) complexes is quenched due to the locking-in of the probe into the protein environment. Interestingly the probe is released from the protein environment in the presence of sodium dodecyl sulfate (SDS) resulting in the restoration of the original luminescence along with a red shift in the emission maximum. These observations are explained in terms of binding constants (Ka) of probe with protein and surfactant and the nature of the binding has been investigated from Scatchard plot and Hill’s coefficient (n) value. These studies point out that the interaction between Re(I) complexes and BSA is cooperative in nature. 相似文献
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Regulation of the PDK4 isozyme by the Rb-E2F1 complex 总被引:1,自引:0,他引:1
Hsieh MC Das D Sambandam N Zhang MQ Nahlé Z 《The Journal of biological chemistry》2008,283(41):27410-27417
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Effect of sesame oil on diuretics or Beta-blockers in the modulation of blood pressure, anthropometry, lipid profile, and redox status 下载免费PDF全文
The study was undertaken to investigate the effect of sesame oil in hypertensive patients who were on antihypertensive therapy either with diuretics (hydrochlorothiazide) or ß-blockers (atenolol). Thirty-two male and 18 female patients aged 35 to 60 years old were supplied sesame oil (Idhayam gingelly oil) and instructed to use it as the only edible oil for 45 days. Blood pressure, anthropometry, lipid profile, lipid peroxidation, and enzymic and non-enzymic antioxidants were measured at baseline and after 45 days of sesame oil substitution. Substitution of sesame oil brought down systolic and diastolic blood pressure to normal. The same patients were asked to withdraw sesame oil consumption for another 45 days, and the measurements were repeated at the end of withdrawal period. Withdrawal of sesame oil substitution brought back the initial blood pressure values. A significant reduction was noted in body weight and body mass index (BMI) upon sesame oil substitution. No significant alterations were observed in lipid profile except triglycerides. Plasma levels of sodium reduced while potassium elevated upon the substitution of sesame oil. Lipid peroxidation (thiobarbituric acid reactive substances [TBARS]) decreased while the activities of superoxide dismutase (SOD), catalase (CAT), and the levels of vitamin C, vitamin E, ß-carotene, and reduced glutathione (GSH) were increased. The results suggested that sesame oil as edible oil lowered blood pressure, decreased lipid peroxidation, and increased antioxidant status in hypertensive patients. 相似文献
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Irisappan Ganesh Sambandam Ravikumar Soon Ho Hong 《Biotechnology and Bioprocess Engineering》2012,17(4):671-678
Currently, a variety of feedstock is utilized by metabolically engineered bacteria for the production of bioenergy and biochemicals. Recent studies have shown that glycerol can be used as an alternative feedstock for glucose, considering its higher availability, lower price, and high degree of reduction. Hence, this review focuses on recent developments in the bioconversion of glycerol to bioenergy (ethanol and hydrogen) and biochemicals (1,3-propanediol, 1,2-propanediol, 3-hydroxypropionic acid, succinic acid, lactic acid, polyhydroxyalkanoates and Lphenyl alanine) using metabolically engineered Escherichia coli. 相似文献
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Ravikumar S Pham VD Lee SH Yoo IK Hong SH 《Journal of industrial microbiology & biotechnology》2012,39(6):861-868
The CusSR two-component system (TCS) is a copper-sensing apparatus of E. coli that is responsible for regulating the copper-related homeostatic system. The dynamic characteristics of the CusSR network were modified by the introduction of a positive feedback loop. To construct the feedback loop, the CusR, which is activated by the cusC promoter, was cloned downstream of the cusC promoter and reporter protein. The feedback loop system, once activated by environmental copper, triggers the activation of the cusC promoter, which results in the amplification of a reporter protein and CusR expression. The threshold copper concentration for the activation of the modified CusSR TCS network was lowered from 2,476.5?μg/l to 247.7?μg/l, which indicates a tenfold increase in sensitivity. The intensity of the output signal was increased twofold, and was maintained for 16?h. The strategy proposed in this study can also be applied to modify the dynamic characteristics of other TCSs. 相似文献
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Senthil Kumar P. Anandan Sambandam Subramanian N. 《International journal of peptide research and therapeutics》2019,25(4):1451-1458
International Journal of Peptide Research and Therapeutics - Mammary gland overexpresses cathepsin D aspartic enzymes during mastitis. Dendrimer (dend)-methoxy poly(ethylene glycol)... 相似文献