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Type 4 pili are dispensable for biofilm development in the cyanobacterium Synechococcus elongatus 下载免费PDF全文
Elad Nagar Shaul Zilberman Eleonora Sendersky Ryan Simkovsky Eyal Shimoni Diana Gershtein Moshe Herzberg Susan S. Golden Rakefet Schwarz 《Environmental microbiology》2017,19(7):2862-2872
The hair‐like cell appendages denoted as type IV pili are crucial for biofilm formation in diverse eubacteria. The protein complex responsible for type IV pilus assembly is homologous with the type II protein secretion complex. In the cyanobacterium Synechococcus elongatus PCC 7942, the gene Synpcc7942_2071 encodes an ATPase homologue of type II/type IV systems. Here, we report that inactivation of Synpcc7942_2071 strongly affected the suite of proteins present in the extracellular milieu (exo‐proteome) and eliminated pili observable by electron microscopy. These results support a role for this gene product in protein secretion as well as in pili formation. As we previously reported, inactivation of Synpcc7942_2071 enables biofilm formation and suppresses the planktonic growth of S. elongatus. Thus, pili are dispensable for biofilm development in this cyanobacterium, in contrast to their biofilm‐promoting function in type IV pili‐producing heterotrophic bacteria. Nevertheless, pili removal is not required for biofilm formation as evident by a piliated mutant of S. elongatus that develops biofilms. We show that adhesion and timing of biofilm development differ between the piliated and non‐piliated strains. The study demonstrates key differences in the process of biofilm formation between cyanobacteria and well‐studied type IV pili‐producing heterotrophic bacteria. 相似文献
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Nathan Stephenson Ryan Perroy Jesse Eiben Frederick Klasner 《Journal of Insect Conservation》2017,21(1):87-96
Determining potentially suitable habitat is critical for effective species conservation and management, but can be challenging in remote or sensitive areas. An approach that combines non-intrusive spatial data collection techniques and supporting field data can lead to a better understanding of landscape-scale species distributions. Here we present two habitat suitability models, at 1 and 10 m resolutions, for the endemic wēkiu bug Nysius wekiuicola, a poorly-understood resident scavenging arthropod species present on the summit of Maunakea in Hawai‘i. Our models reveal that the wēkiu bug, restricted almost entirely to portions of cinder cones above 3500 m elevation, has a high degree of habitat specificity and represents a classically rare species. Across the 55 km2 study area, 850 ha of potentially suitable habitat were identified at the 10 percentile training threshold, with the core area located at the true summit. Our results show that elevation and surficial mineralogy were the strongest predictors of suitable habitat, with lesser contributions from aspect and slope. Climatic variables also likely influence wēkiu bug distribution patterns, but were not included in our models due to the coarseness of available climate data and high correlation between variables. Relatively minor differences between the two models, in terms of identifying the locations and amount of suitable wēkiu bug habitat, and a higher measure of performance for the 10 m resolution model, suggest that coarser resolution input variables may characterize suitable habitat more efficiently than very fine 1 m resolution data. The suitability models generated as a result of this study will be directly incorporated into conservation management and restoration goals, and can easily be adapted for other arthropod species, leading to a more holistic understanding of metacommunity dynamics at the Maunakea summit. 相似文献
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Ryan J. Haasl Bret A. Payseur 《Evolution; international journal of organic evolution》2014,68(9):2737-2744
Long dinucleotide repeats found in exons present a substantial mutational hazard: mutations at these loci occur often and generate frameshifts. Here, we provide clear and compelling evidence that exonic dinucleotides experience strong selective constraint. In humans, only 18 exonic dinucleotides have repeat lengths greater than six, which contrasts sharply with the genome‐wide distribution of dinucleotides. We genotyped each of these dinucleotides in 200 humans from eight 1000 Genomes Project populations and found a near‐absence of polymorphism. More remarkably, divergence data demonstrate that repeat lengths have been conserved across the primate phylogeny in spite of what is likely considerable mutational pressure. Coalescent simulations show that even a very low mutation rate at these loci fails to explain the anomalous patterns of polymorphism and divergence. Our data support two related selective constraints on the evolution of exonic dinucleotides: a short‐term intolerance for any change to repeat length and a long‐term prevention of increases to repeat length. In general, our results implicate purifying selection as the force that eliminates new, deleterious mutants at exonic dinucleotides. We briefly discuss the evolution of the longest exonic dinucleotide in the human genome—a 10 x CA repeat in fibroblast growth factor receptor‐like 1 (FGFRL1)—that should possess a considerably greater mutation rate than any other exonic dinucleotide and therefore generate a large number of deleterious variants. 相似文献
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The Amazonian catfish, Panaque nigrolineatus, consume large amounts of wood in their diets. The nitrogen-fixing community within the gastrointestinal (GI) tract of these catfish was found to include nifH phylotypes that are closely related to Clostridium sp., Alpha and Gammaproteobacteria, and sequences associated with GI tracts of lower termites. Fish fed a diet of sterilized palm wood were found to contain nifH messenger RNA within their GI tracts, displaying high sequence similarity to the nitrogen-fixing Bradyrhizobium group. Nitrogenase activity, measured by acetylene reduction assays, could be detected in freshly dissected GI tract material and also from anaerobic enrichment cultures propagated in nitrogen-free enrichment media; nifH sequences retrieved from these cultures were dominated by Klebsiella- and Clostridium-like sequences. Microscopic examination using catalyzed reporter deposition-enhanced immunofluorescence revealed high densities of nitrogenase-containing cells colonizing the woody digesta within the GI tract, as well as cells residing within the intestinal mucous layer. Our findings suggest that the P. nigrolineatus GI tract provides a suitable environment for nitrogen fixation that may facilitate production of reduced nitrogen by the resident microbial population under nitrogen limiting conditions. Whether this community is providing reduced nitrogen to the host in an active or passive manner and whether it is present in a permanent or transient relationship remains to be determined. The intake of a cellulose rich diet and the presence of a suitable environment for nitrogen fixation suggest that the GI tract microbial community may allow a unique trophic niche for P. nigrolineatus among fish. 相似文献
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Even species within the same assemblage have varied responses to climate change, and there is a poor understanding for why some taxa are more sensitive to climate than others. In addition, multiple mechanisms can drive species' responses, and responses may be specific to certain life stages or times of year. To test how marine species respond to climate variability, we analyzed 73 diverse taxa off the southeast US coast in 26 years of scientific trawl survey data and determined how changes in distribution and biomass relate to temperature. We found that winter temperatures were particularly useful for explaining interannual variation in species' distribution and biomass, although the direction and magnitude of the response varied among species from strongly negative, to little response, to strongly positive. Across species, the response to winter temperature varied greatly, with much of this variation being explained by thermal preference. A separate analysis of annual commercial fishery landings revealed that winter temperatures may also impact several important fisheries in the southeast United States. Based on the life stages of the species surveyed, winter temperature appears to act through overwinter mortality of juveniles or as a cue for migration timing. We predict that this assemblage will be responsive to projected increases in temperature and that winter temperature may be broadly important for species relationships with climate on a global scale. 相似文献
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John F. E. Newman Patricia G. Piatti Martin D. Ryan Fred Brown 《Trends in microbiology》1994,2(12):494-498
Foot-and-mouth disease virus and poliovirus each contain several minor polypeptides, in addition to the four structural proteins. One of these, the viral RNA polymerase, can also act as a nuclease, hydrolysing the RNA and thus destroying viral infectivity. It is tightly bound to the RNA and may be the packaging signal for assembly of the particle. 相似文献