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排序方式: 共有259条查询结果,搜索用时 187 毫秒
1.
Sacramento Doralice Rodrigues Coelho Rosalie Reed Rodrigues Wigg Márcia Dutra Toledo Luna Linhares Luiz Fernando de Matos dos Santos Marta Gonçalves Azevedo Soares Semêdo Luzia Teixeira de Ribeiro da Silva Antonio Jorge 《World journal of microbiology & biotechnology》2004,20(3):225-229
A promising producer of bioactive compounds isolated from a Brazilian tropical soil was tested for its range of antimicrobial
activities. Strain 606, classified as Streptomyces sp., could not be identified up to species level, suggesting a possible new taxon. The supernatant and 10 extracts and fractions,
obtained by extraction and chromatographic techniques, presented antimicrobial activity using antibiograms. The methanolic
fraction was highly active against pathogenic bacteria, phytopathogenic fungi and the human pathogenic yeast Candida albicans. It also possessed high antiviral activity inhibiting the propagation of an acyclovir-resistant herpes simplex virus type
1 strain on HEp-2 cells at non-cytotoxic concentration. The strong cytotoxic effect suggests an antitumour action.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
2.
Naveen Parmar Kyle Burrows Pia M. Vornewald Hvard T. Lindholm Rosalie T. Zwiggelaar Alberto Díez-Snchez Mara Martín-Alonso Madeleine Fosslie Bruce A. Vallance John Arne Dahl Colby Zaph Menno J. Oudhoff 《PLoS pathogens》2021,17(3)
Infectious and inflammatory diseases in the intestine remain a serious threat for patients world-wide. Reprogramming of the intestinal epithelium towards a protective effector state is important to manage inflammation and immunity and can be therapeutically targeted. The role of epigenetic regulatory enzymes within these processes is not yet defined. Here, we use a mouse model that has an intestinal-epithelial specific deletion of the histone demethylase Lsd1 (cKO mice), which maintains the epithelium in a fixed reparative state. Challenge of cKO mice with bacteria-induced colitis or a helminth infection model both resulted in increased pathogenesis. Mechanistically, we discovered that LSD1 is important for goblet cell maturation and goblet-cell effector molecules such as RELMß. We propose that this may be in part mediated by directly controlling genes that facilitate cytoskeletal organization, which is important in goblet cell biology. This study therefore identifies intestinal-epithelial epigenetic regulation by LSD1 as a critical element in host protection from infection. 相似文献
3.
Respondents to a respiratory survey of Berlin, New Hampshire, residents in 1961 have been studied to assess the relationship between co-operation and respiratory disease prevalence. Two hundred and forty-three unco-operative subjects, interviewed at home, had significantly more morning phlegm and a lower vital capacity than carefully matched subjects who attended the central clinic. Fifty-one volunteers had the same prevalence of respiratory disease symptoms and physiological abnormalities as carefully matched subjects drawn from a probability sample of the city.It is concluded that respiratory disease prevalence will be underestimated if calculated from studies of co-operative subjects who attend a clinic. Case-finding by respiratory disease screening clinics will also miss many persons who suffer from chronic bronchitis. 相似文献
4.
Marcella Willemsen Rugile Linkut Rosalie M. Luiten Tiago R. Matos 《Pigment cell & melanoma research》2019,32(5):612-622
Tissue‐resident memory T (TRM) cells are abundant in the memory T cell pool and remain resident in peripheral tissues, such as the skin, where they act as alarm sensors or cytotoxic killers. TRM cells persist long after the pathogen is eliminated and can respond rapidly upon reinfection with the same antigen. When aberrantly activated, skin‐located TRM cells have a profound role in various skin disorders, including vitiligo and melanoma. Autoreactive TRM cells are present in human lesional vitiligo skin and mouse models of vitiligo, which suggests that targeting these cells could be effective as a durable treatment strategy for vitiligo. Furthermore, emerging evidence indicates that induction of melanoma‐reactive TRM cells is needed to achieve effective protection against tumor growth. This review highlights seminal reports about skin‐resident T cells, focusing mainly on their role in the context of vitiligo and melanoma, as well as their potential as therapeutic targets in both diseases. 相似文献
5.
Rishikesh Pandey Renjie Zhou Rosalie Bordett Ciera Hunter Kristine Glunde Ishan Barman Tulio Valdez Christine Finck 《Journal of biophotonics》2019,12(4)
Label‐free quantitative imaging is highly desirable for studying live cells by extracting pathophysiological information without perturbing cell functions. Here, we demonstrate a novel label‐free multimodal optical imaging system with the capability of providing comprehensive morphological and molecular attributes of live cells. Our morpho‐molecular microscopy (3M) system draws on the combined strength of quantitative phase microscopy (QPM) and Raman microscopy to probe the morphological features and molecular fingerprinting characteristics of each cell under observation. While the commonr‐path geometry of our QPM system allows for highly sensitive phase measurement, the Raman microscopy is equipped with dual excitation wavelengths and utilizes the same detection and dispersion system, making it a distinctive multi‐wavelength system with a small footprint. We demonstrate the applicability of the 3M system by investigating nucleated and nonnucleated cells. This integrated label‐free platform has a promising potential in preclinical research, as well as in clinical diagnosis in the near future. 相似文献
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Marcella Willemsen Gabrielle Krebbers Marcel W. Bekkenk Marcel B.M. Teunissen Rosalie M. Luiten 《The journal of histochemistry and cytochemistry》2021,69(5):339
The Opal multiplex technique is an established methodology for the detection of multiple biomarkers in one section. The protocol encompasses iterative single stainings and heating-mediated removal of the primary and secondary antibodies after each staining round, leaving untouched the Opal fluorophores which are deposited onto the antigen of interest. According to our experience, repetitive heating of skin sections often results in tissue damage, indicating an urgent need for milder alternatives to strip immunoglobulins. In this study, we demonstrate that considerable heating-related damage was found not only in skin but also in tissues of different origin, mostly characterized by low cell density. Importantly, the morphology remained fully intact when sections were repetitively exposed to β-mercaptoethanol-containing stripping buffer instead of multiple heating cycles. However, target epitopes appeared sensitive at a differential degree to multiple treatments with stripping buffer, as shown by loss in staining intensity, but in all cases, the staining intensity could be restored by increment of the primary antibody concentrations. Application of β-mercaptoethanol-containing stripping buffer instead of heating for antibody removal markedly improved the quality of the Opal multiplex technique, as a substantial higher number of differently colored cells could be visualized within a well-conserved morphological context: 相似文献
9.
Pesticide ecotoxicological effect factors and their uncertainties for freshwater ecosystems 总被引:1,自引:1,他引:0
Rosalie van Zelm Mark A. J. Huijbregts Leo Posthuma Arjen Wintersen Dik van de Meent 《The International Journal of Life Cycle Assessment》2009,14(1):43-51
Background, aim, and scope Characterization factors for ecotoxicity in the Life Cycle Impact Assessment (LCIA) are used to convert emissions into ecotoxicological
impacts. Deriving them involves a fate and an effect analysis step. The fate factor quantifies the change in environmental
concentration per unit of emission, while the effect factor quantifies the change in impact on the ecosystem per unit of environmental
concentration. This paper calculates freshwater ecotoxicological effect factors for 397 pesticides belonging to 11 pesticide-specific
toxic modes of action (TMoA), such as acetylcholinesterase inhibition and photosynthesis inhibition. Moreover, uncertainties
in the effect factors due to uncertain background concentrations and due to limited toxicity data are quantified.
Methods To calculate median ecotoxicological effect factors (EEFs), toxic pressure assessments were made, based on the species sensitivity
distribution—and the multisubstance potentially affected fraction—concept. The EEF quantifies an estimate of the fraction
of species that is probably affected due to a marginal change in concentration of a pesticide. EEFs were divided into a TMoA-specific
and a chemical-specific part, which were calculated on the basis of physicochemical properties, emissions, and toxicity data.
Propagation of parameter uncertainty in the EEFs and the TMoA- and chemical-specific parts was quantified by Monte Carlo simulation
and results were reported as 90% confidence intervals.
Results Median EEFs range from 2·10−3 to 7·106 l/g. Uncertainty in the TMoA-specific part is dominated by uncertainty in the TMoA-specific spread in species sensitivity
and by uncertainty in the effective toxicity of a TMoA. Uncertainty in the chemical-specific part of the EEFs depends on the
number of species for which toxicity data are available to calculate average toxicity (n
s) and ranges from a median uncertainty of 2.6 orders of magnitude for n
s = 2 to one order of magnitude for n
s ≥ 4. The TMoA-specific effect factor for systemic fungicides shows the largest uncertainty range. For seven TMoAs, uncertainty
ranges of the TMoA-specific effect factor are less than two orders of magnitude. For the other four TMoAs, the EEF uncertainty
range is between two and eight orders of magnitude. For the chemical-specific part of the EEFs, we found that variation in
uncertainty readily decreases for pesticides for which toxicity data are available for at least three species.
Discussion The same parameters that contributed most to uncertainty were found for pesticides as were found before for high-production-volume
chemicals. However, uncertainty in concentrations of pesticides was lower. TMoA-specific factors obtained with the applied
nonlinear method differ up to nine orders of magnitude from the factor of 0.5, which is used in the linear method. With the
applied method, a distinction in EEFs can be made among different TMoAs.
Conclusions Ecotoxicological effect factors are presented, including overviews of their uncertainty ranges and the main contributors to
uncertainty. The applied nonlinear method provides the possibility to quantify parameter uncertainty in the TMoA-specific
part of the ecotoxicological effect factor, which is helpful to get more insight in how uncertainty in ecotoxicological characterization
factors can be reduced.
Recommendations and perspectives The calculated uncertainty ranges can be included in life cycle assessment (LCA) case studies, which allows for better interpretation
of LCA results obtained with the EEFs. To put the uncertainty in effect factors into perspective within LCIA, more information
on the uncertainty in fate factors should be derived.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
10.