全文获取类型
收费全文 | 2193篇 |
免费 | 147篇 |
出版年
2023年 | 18篇 |
2022年 | 16篇 |
2021年 | 47篇 |
2020年 | 45篇 |
2019年 | 36篇 |
2018年 | 51篇 |
2017年 | 50篇 |
2016年 | 74篇 |
2015年 | 118篇 |
2014年 | 148篇 |
2013年 | 175篇 |
2012年 | 187篇 |
2011年 | 164篇 |
2010年 | 90篇 |
2009年 | 99篇 |
2008年 | 127篇 |
2007年 | 107篇 |
2006年 | 112篇 |
2005年 | 85篇 |
2004年 | 80篇 |
2003年 | 80篇 |
2002年 | 68篇 |
2001年 | 20篇 |
2000年 | 14篇 |
1999年 | 29篇 |
1998年 | 20篇 |
1997年 | 18篇 |
1996年 | 19篇 |
1995年 | 23篇 |
1994年 | 21篇 |
1993年 | 16篇 |
1992年 | 22篇 |
1991年 | 14篇 |
1990年 | 22篇 |
1989年 | 10篇 |
1988年 | 11篇 |
1987年 | 6篇 |
1986年 | 14篇 |
1985年 | 5篇 |
1984年 | 12篇 |
1982年 | 6篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 5篇 |
1974年 | 6篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1971年 | 3篇 |
排序方式: 共有2340条查询结果,搜索用时 15 毫秒
1.
Cem Sievers Tommy Schlumpf Ritwick Sawarkar Federico Comoglio Renato Paro 《Nucleic acids research》2012,40(20):e160
The Photo-Activatable Ribonucleoside-enhanced CrossLinking and ImmunoPrecipitation (PAR-CLIP) method was recently developed for global identification of RNAs interacting with proteins. The strength of this versatile method results from induction of specific T to C transitions at sites of interaction. However, current analytical tools do not distinguish between non-experimentally and experimentally induced transitions. Furthermore, geometric properties at potential binding sites are not taken into account. To surmount these shortcomings, we developed a two-step algorithm consisting of a non-parametric two-component mixture model and a wavelet-based peak calling procedure. Our algorithm can reduce the number of false positives up to 24% thereby identifying high confidence interaction sites. We successfully employed this approach in conjunction with a modified PAR-CLIP protocol to study the functional role of nuclear Moloney leukemia virus 10, a putative RNA helicase interacting with Argonaute2 and Polycomb. Our method, available as the R package wavClusteR, is generally applicable to any substitution-based inference problem in genomics. 相似文献
2.
Joana Darc Souza Chaves Jaqueline Lopes Damasceno Marcela Cristina Ferreira Paula Pollyanna Francielli de Oliveira Gustavo Chevitarese Azevedo Renato Camargo Matos Maria Cristina S. Lourenço Denise Crispim Tavares Heveline Silva Ana Paula Soares Fontes Mauro Vieira de Almeida 《Biometals》2015,28(5):845-860
3.
4.
5.
6.
7.
8.
Glucoraphasatin is an atypical glucosinolate mainly found in Raphanus sativus roots and sprouts. This review focuses on the chemistry, the occurrence, and the biological properties of glucoraphasatin. 相似文献
9.
10.
Antonio Hernandes Chaves Neto Karla Cristiana Queiroz Renato Milani Edgar Julian Paredes‐Gamero Giselle Zenker Justo Maikel P. Peppelenbosch Carmen Veríssima Ferreira 《Journal of cellular biochemistry》2011,112(1):71-77
Despite numerous reports on the ability of ascorbic acid and β‐glycerophosphate (AA/β‐GP) to induce osteoblast differentiation, little is known about the molecular mechanisms involved in this phenomenon. In this work, we used a peptide array containing specific consensus sequences (potential substrates) for protein kinases and traditional biochemical techniques to examine the signaling pathways modulated during AA/β‐GP‐induced osteoblast differentiation. The kinomic profile obtained after 7 days of treatment with AA/β‐GP identified 18 kinase substrates with significantly enhanced or reduced phosphorylation. Peptide substrates for Akt, PI3K, PKC, BCR, ABL, PRKG1, PAK1, PAK2, ERK1, ERBB2, and SYK showed a considerable reduction in phosphorylation, whereas enhanced phosphorylation was observed in substrates for CHKB, CHKA, PKA, FAK, ATM, PKA, and VEGFR‐1. These findings confirm the potential usefulness of peptide microarrays for identifying kinases known to be involved in bone development in vivo and in vitro and show that this technique can be used to investigate kinases whose function in osteoblastic differentiation is poorly understood. J. Cell. Biochem. 112: 71–77, 2011. © 2010 Wiley‐Liss, Inc. 相似文献