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排序方式: 共有652条查询结果,搜索用时 15 毫秒
1.
Suzanne Camus Sergio Menéndez Kenneth Fernandes Nelly Kua Geng Liu Dimitris P. Xirodimas David P. Lane Jean-Christophe Bourdon 《Cell cycle (Georgetown, Tex.)》2012,11(8):1646-1655
The discovery that the single p53 gene encodes several different p53 protein isoforms has initiated a flurry of research into the function and regulation of these novel p53 proteins. Full-length p53 protein level is primarily regulated by the E3-ligase Mdm2, which promotes p53 ubiquitination and degradation. Here, we report that all of the novel p53 isoforms are ubiquitinated and degraded to varying degrees in an Mdm2-dependent and -independent manner, and that high-risk human papillomavirus can degrade some but not all of the novel isoforms, demonstrating that full-length p53 and the p53 isoforms are differentially regulated. In addition, we provide the first evidence that Mdm2 promotes the NEDDylation of p53β. Altogether, our data indicates that Mdm2 can distinguish between the p53 isoforms and modify them differently. 相似文献
2.
Six bisindole alkaloids have been isolated from the leaves of Dyera costulata (Apocynaceae). One is the known ochrolifuanine A and the others are the novel ochrolifuanines E and F, and the 18-dehydroochrolifuanines A, E and F. 相似文献
3.
Valeriy A. Sterligov Iaroslav O. Grytsaienko Yakiv Men Maxim L. Nesterov Alexey Yu. Nikitin 《Plasmonics (Norwell, Mass.)》2014,9(2):219-226
The enhancement factor for surface plasmon–polaritons scattering by a thin dielectric grating was measured experimentally. Scattering of a p-polarized wave may be up to 30,000 times stronger than the non-resonant scattering of an s-polarized wave by the same grating. A detailed comparison between the theoretical calculations and experimental measurements was performed. Strong localization of the scattered field near the edges of diffraction grating grooves was found. Such localization is very promising for numerous applications, e.g., biological sensors, optical tweezers for catching particles, or viruses, etc. 相似文献
4.
Knockdown of mediator subunit Med19 suppresses bladder cancer cell proliferation and migration by downregulating Wnt/β‐catenin signalling pathway 下载免费PDF全文
Changping Men Diandong Yang Zhenli Gao Zhe Zhu Jitao Wu 《Journal of cellular and molecular medicine》2017,21(12):3254-3263
Mediator complex subunit 19 (Med19), a RNA polymerase II‐embedded coactivator, is reported to be involved in bladder cancer (BCa) progression, but its functional contribution to this process is poorly understood. Here, we investigate the effects of Med19 on malignant behaviours of BCa, as well as to elucidate the possible mechanisms. Med19 expression in 15 BCa tissues was significantly higher than adjacent paired normal tissues using real‐time PCR and Western blot analysis. Immunohistochemical staining of 167 paraffin‐embedded BCa tissues was performed, and the results showed that high Med19 protein level was positively correlated with clinical stages and histopathological grade. Med19 was knocked down in BCa cells using short‐hairpin RNA. Functional assays showed that knocking‐down of Med19 can suppress cell proliferation and migration in T24, UM‐UC3 cells and 5637 in vitro, and inhibited BCa tumour growth in vivo. TOP/FOPflash reporter assay revealed that Med19 knockdown decreased the activity of Wnt/β‐catenin pathway, and the target genes of Wnt/β‐catenin pathway were down‐regulated, including Wnt2, β‐catenin, Cyclin‐D1 and MMP‐9. However, protein levels of Gsk3β and E‐cadherin were elevated. Our data suggest that Med19 expression correlates with aggressive characteristics of BCa and Med19 knockdown suppresses the proliferation and migration of BCa cells through down‐regulating the Wnt/β‐catenin pathway, thereby highlighting Med19 as a potential therapeutic target for BCa treatment. 相似文献
5.
Reference values for TSH may be inadequate to define hypothyroidism in persons with morbid obesity: Di@bet.es study 下载免费PDF全文
Sergio Valdés Cristina Maldonado‐Araque Ana Lago‐Sampedro Juan Antonio Lillo‐Muñoz Eduardo Garcia‐Fuentes Vidal Perez‐Valero Carolina Gutiérrez‐Repiso Eva Garcia‐Escobar Albert Goday Inés Urrutia Laura Peláez Alfonso Calle‐Pascual Elena Bordiú Luis Castaño Conxa Castell Elias Delgado Edelmiro Menéndez Josep Franch‐Nadal Sonia Gaztambide Joan Girbés Emilio Ortega Joan Vendrell Matilde R. Chacón F. Javier Chaves Federico Soriguer Gemma Rojo‐Martínez 《Obesity (Silver Spring, Md.)》2017,25(4):788-793
6.
O Domínguez-López A Fernández-Riestra B Marín A Menéndez-Patterson 《Revista Espanola de Fisiología》1985,41(2):151-159
The effects of interaction of the thyroid function with the submaxillary glands on the female rat, on the body growth, weight evolution of adrenal glands, ovary and parotid glands, puberty onset, estrous cycle, fertility, gestation and offspring size, plasmatic levels of glucose, glucagon, Na+, K+ and total protein have been studied. The effects of sialadenectomy and thyroid function are both independent in all parameters, except in ovary growth, which is depressed by thyroid function when submaxillary glands are removed, and in circulating glucose, since sialadenectomy stabilizes to hypoglycemia which results from thyroidectomy. 相似文献
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9.
Jiangang Yang Chaoyu Tian Tong Zhang Chenxi Ren Yueming Zhu Yan Zeng Yan Men Yuanxia Sun Yanhe Ma 《Biotechnology and bioengineering》2019,116(4):745-756
D -Allulose 3-epimerase (DAE) has been applied to produce D -allulose, a low-calorie and functional sweetener. In this study, a new DAE from Paenibacillus senegalensis was characterized in Escherichia coli. Furthermore, we presented a tandem isoenzyme gene expression strategy to express multiple DAEs in one cell and construct food-grade expression systems based on Corynebacterium glutamicum. Seventeen expression cassettes based on three DAE genes from different organisms were constructed. Among all recombinant strains, DAE16 harboring three DAE genes in an expression vector exhibited the highest enzyme activity with 22.7 U/mg. Whole-cell transformation of DAE16 produced 225 g/L D -allulose with a volumetric productivity of 353 g·g −1·hr −1. The catalytic efficiency of strain C-DAE9 integrating total 11 DAE genes in chromosome was 16.4-fold higher than strains carrying one DAE. Fed-batch culture of C-DAE9 gave enzyme activity of 44,700 U/L. We also expressed a thermostable invertase in C. glutamicum and obtained enzyme activity of 29 U/mg. Immobilized cells expressing DAE or invertase exhibited 80% of retained activity after 30 cycles of catalytic reactions. Those immobilized cells were coupled to produce 61.2 g/L D -allulose from cane molasses in a two-step reaction process. This study provided an efficient approach for enzyme preparation and allowed access to produce D -allulose from other abundant and low-cost feedstock enriched with sucrose. 相似文献
10.
Manfred Accrombessi Sma?la Ouédraogo Gino Cédric Agbota Raquel Gonzalez Achille Massougbodji Clara Menéndez Michel Cot 《PloS one》2015,10(6)