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Oligonucleotide phosphorothioates have been synthesized using procaryotic DNA polymerase and oligonucleotide template/primer.
The method facilitates the recovery of DNA polymerase and template/primer and is successful at the milligram scale.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
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Recombinant monoclonal antibodies (mAbs) have become an important category of biological therapeutics. mAbs share the same structures and biological functions as endogenous IgG molecules. One function is complement-dependent cytotoxicity (CDC) initiation by binding of C1q. Traditionally, ELISA methods have been utilized to measure C1q binding. A new robust capture method was established in this study to measure the binding affinity of C1q to antibodies by surface plasmon resonance (SPR). The utility of this method was demonstrated by determination of the difference in IgG subclass specificity of C1q binding. 相似文献
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Priya Gogoi Saedeh Sepehri Yi Zhou Michael A. Gorin Carmela Paolillo Ettore Capoluongo Kyle Gleason Austin Payne Brian Boniface Massimo Cristofanilli Todd M. Morgan Paolo Fortina Kenneth J. Pienta Kalyan Handique Yixin Wang 《PloS one》2016,11(1)
Current analysis of circulating tumor cells (CTCs) is hindered by sub-optimal sensitivity and specificity of devices or assays as well as lack of capability of characterization of CTCs with clinical biomarkers. Here, we validate a novel technology to enrich and characterize CTCs from blood samples of patients with metastatic breast, prostate and colorectal cancers using a microfluidic chip which is processed by using an automated staining and scanning system from sample preparation to image processing. The Celsee system allowed for the detection of CTCs with apparent high sensitivity and specificity (94% sensitivity and 100% specificity). Moreover, the system facilitated rapid capture of CTCs from blood samples and also allowed for downstream characterization of the captured cells by immunohistochemistry, DNA and mRNA fluorescence in-situ hybridization (FISH). In a subset of patients with prostate cancer we compared the technology with a FDA-approved CTC device, CellSearch and found a higher degree of sensitivity with the Celsee instrument. In conclusion, the integrated Celsee system represents a promising CTC technology for enumeration and molecular characterization. 相似文献
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A 15-year-old female rhesus monkey was observed to have bilaterally thinned and prominently curved corneas. Slit lamp observations, pachymetry, keratometry, and corneoscopy were consistent with a diagnosis of keratoconus, a relatively common corneal dystrophy in humans heretofore not described in a subhuman primate. 相似文献
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Besides vobtusine and vobtusine-lactone, deoxyvobtusine was isolated from the leaves of Voacanga grandifolia (Miq. Rolfe. Spectral and chemical evi 相似文献