全文获取类型
收费全文 | 1421篇 |
免费 | 157篇 |
出版年
2021年 | 16篇 |
2020年 | 11篇 |
2019年 | 12篇 |
2018年 | 14篇 |
2017年 | 14篇 |
2016年 | 33篇 |
2015年 | 32篇 |
2014年 | 55篇 |
2013年 | 56篇 |
2012年 | 65篇 |
2011年 | 72篇 |
2010年 | 50篇 |
2009年 | 39篇 |
2008年 | 70篇 |
2007年 | 64篇 |
2006年 | 50篇 |
2005年 | 59篇 |
2004年 | 58篇 |
2003年 | 47篇 |
2002年 | 49篇 |
2001年 | 38篇 |
2000年 | 50篇 |
1999年 | 40篇 |
1998年 | 20篇 |
1997年 | 14篇 |
1995年 | 11篇 |
1994年 | 16篇 |
1992年 | 37篇 |
1991年 | 39篇 |
1990年 | 38篇 |
1989年 | 28篇 |
1988年 | 31篇 |
1987年 | 24篇 |
1986年 | 25篇 |
1985年 | 21篇 |
1984年 | 16篇 |
1983年 | 16篇 |
1982年 | 7篇 |
1981年 | 16篇 |
1980年 | 12篇 |
1979年 | 17篇 |
1978年 | 22篇 |
1976年 | 12篇 |
1975年 | 13篇 |
1974年 | 13篇 |
1973年 | 17篇 |
1971年 | 20篇 |
1970年 | 7篇 |
1969年 | 10篇 |
1968年 | 10篇 |
排序方式: 共有1578条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
5.
Free alpha-like material from bovine pituitaries. Removal of its O-linked oligosaccharide permits combination with lutropin-beta 总被引:2,自引:0,他引:2
Further characterization of the free alpha subunit immunoreactive material, not combined with beta subunit in extracts of bovine pituitaries, shows that the only significant modifications, relative to alpha subunits themselves, are the oligosaccharide O-linked to threonine-43, and heterogeneity of the carboxyl terminus. Removal of the O-linked carbohydrate with a mixture of glycosidases from Streptococcus pneumoniae results in an alpha-like material capable of combining with lutropin beta subunit and, thus, the presence of the oligosaccharide is responsible for the inability of the free alpha-like material to combine with beta subunits. Amino acid compositions of tryptic peptides spanning the entire sequence indicate no change in amino acid sequence of the free alpha-like material as compared to lutropin alpha. Further, based on the similar behavior reverse phase high performance liquid chromatography of the tryptic peptides as compared to their lutropin alpha counterparts, it is concluded that no additional post-translational modifications are present. The N-linked oligosaccharides of the free alpha-like material most likely contain terminal O-sulfated N-acetylhexosamines (as do the asparagine-linked carbohydrates from the pituitary hormones) as indicated by the presence of 3 mol of sulfate/mol of free alpha-like material and the resistance of these oligosaccharides to enzymatic deglycosylation. The O-linked oligosaccharide does not contain sulfated residues. 相似文献
6.
Effects of menstrual cycle on metabolic responses to exercise 总被引:1,自引:0,他引:1
7.
8.
9.
D R Webb K Mason G Semenuk T M Aune C W Pierce 《Journal of immunology (Baltimore, Md. : 1950)》1985,135(5):3238-3242
Soluble immune response suppressor (SIRS) isolated from the T cell hybrid 393D2.6 was originally reported to exist as at least two m.w. forms and to migrate on reverse-phase high-performance liquid chromatography columns as three separate species. In experiments presented here, a further analysis of the different chromatographic forms of SIRS has been carried out. SIRS-alpha elutes from C-18 reverse-phase columns in 20% propanol. When SIRS-alpha is subjected to isoelectric focusing, three biologically active species are isolated at approximately pH7, approximately pH6, and approximately pH5 (SIRS-alpha 7, SIRS-alpha 6, and SIRS-alpha 5, respectively). SIRS-beta elutes in 30% propanol, and on isoelectric focusing the biologic activity is found only at approximately pH7 (SIRS-beta 7). Both the alpha and beta forms of SIRS have nearly identical m.w. when subjected to molecular sieve chromatography and migrate with a m.w. of 11,000. The molecular basis for these isoforms is not yet clear but is consistent with earlier studies showing two separate messenger RNA species coding for SIRS. 相似文献
10.
Identification of the insulin receptor tyrosine residues undergoing insulin-stimulated phosphorylation in intact rat hepatoma cells 总被引:8,自引:0,他引:8
H E Tornqvist J R Gunsalus R A Nemenoff A R Frackelton M W Pierce J Avruch 《The Journal of biological chemistry》1988,263(1):350-359
Tyr(P)-containing proteins were purified from extracts of insulin-treated rat hepatoma cells (H4-II-E-C3) by antiphosphotyrosine immunoaffinity chromatography. Two major insulin-stimulated, Tyr(P) proteins were recovered: an Mr 95,000 protein (identified as the insulin receptor beta subunit by its immunoprecipitation by a patient-derived anti-insulin receptor serum and several anti-insulin receptor (peptide) antisera) and an Mr 180,000 protein (which was unreactive with all anti-insulin receptor antibodies). After purification and tryptic digestion of the Mr 95,000 protein, tryptic peptides containing Tyr(P) were purified by sequential antiphosphotyrosine immunoaffinity, reversed-phase, anion-exchange chromatography. The partial amino acid sequence obtained by gas- and solid-phase Edman degradation was compared to the amino acid sequence of the intracellular extension of the rat insulin receptor deduced from the genomic sequence. Approximately 80% of all beta subunit [32P]Tyr(P) resides on two tryptic peptides: 50-60% of [32P]Tyr(P) is found on the tryptic peptide Asp-Ile-Tyr-Glu-Thr-Asp-Tyr-Tyr-Arg from the tyrosine kinase domain, which is recovered mainly as the double phosphorylated species (predominantly in the form with Tyr(P) at residues 3 and 7 from the amino terminus; the remainder with Tyr(P) at residues 3 and 8), with 10-15% as the triple phosphorylated species. A second tryptic peptide is located near the carboxyl terminus, contains 2 tyrosines, and has the sequence, Thr-Tyr-Asp-Glu-His-Ile-Pro-Tyr-Thr-; this contains 20-30% of beta subunit [32P]Tyr(P) and is identified primarily in a double phosphorylated form. Approximately 10% of beta subunit [32P]Tyr(P) resides on an unidentified tryptic peptide of Mr 4,000-5,000. The insulin-stimulated tyrosine phosphorylation of the insulin receptor in intact rat hepatoma cells thus involves at least 6 of the 13 tyrosine residues located on the beta subunit intracellular extension. These tyrosines are clustered in several domains in a distribution virtually identical to that previously found for partially purified human insulin receptor autophosphorylated in vitro in the presence of insulin. This multisite regulatory tyrosine phosphorylation is the initial intracellular event in insulin action. 相似文献