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Matthew Glick Perry BiddleJosh Jantzi Samantha WeaverDoug Schirch 《Biochemical and biophysical research communications》2014
Clinical research is currently exploring the validity of the anti-tumor candidate 3-bromopyruvate (3-BP) as a novel treatment for several types of cancer. However, recent publications have overlooked rarely-cited earlier work about the instability of 3-BP and its decay to 3-hydroxypyruvate (3-HP) which have obvious implications for its mechanism of action against tumors, how it is administered, and for precautions when preparing solutions of 3-BP. This study found the first-order decay rate of 3-BP at physiological temperature and pH has a half-life of only 77 min. Lower buffer pH decreases the decay rate, while choice of buffer and concentration do not affect it. A method for preparing more stable solutions is also reported. 相似文献
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Cobalamin inactivation induces formyltetrahydrofolate synthetase 总被引:1,自引:0,他引:1
Loss of cobalamin function produces profound changes in the metabolism of formate. There is impaired synthesis of formyltetrahydropteroylglutamate synthetase (CHO-H4PteGlu), accumulation of endogenous formate and impaired utilization of [14C]formate. There are contradictory reports on the effect of cobalamin inactivation on CHO-H4PteGlu synthetase. This study confirms a significant increase in synthetase activity following cobalamin inactivation. 相似文献
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Perry S Brignola Karen Lackey Sue H Kadwell Christine Hoffman Earnest Horne H Luke Carter J Darren Stuart Kevin Blackburn Mary B Moyer Krystal J Alligood Wilson B Knight Edgar R Wood 《The Journal of biological chemistry》2002,277(2):1576-1585
Epidermal growth factor receptor (EGFR), ErbB-2, and ErbB-4 are members of the type 1 receptor tyrosine kinase family. Overexpression of these receptors, especially ErbB-2 and EGFR, has been implicated in multiple forms of cancer. Inhibitors of EGFR tyrosine kinase activity are being evaluated clinically for cancer therapy. The potency and selectivity of these inhibitors may affect the efficacy and toxicity of therapy. Here we describe the expression, purification, and biochemical comparison of EGFR, ErbB-2, and ErbB-4 intracellular domains. Despite their high degree of sequence homology, the three enzymes have significantly different catalytic properties and substrate kinetics. For example, the catalytic activity of ErbB-2 is less stable than that of EGFR. ErbB-2 uses ATP-Mg as a substrate inefficiently compared with EGFR and ErbB-4. The three enzymes have very similar substrate preferences for three optimized peptide substrates, but differences in substrate synergies were observed. We have used the biochemical and kinetic parameters determined from these studies to develop an assay system that accurately measures inhibitor potency and selectivity between the type 1 receptor family. We report that the selectivity profile of molecules in the 4-anilinoquinazoline series can be modified through specific aniline substitutions. Moreover, these compounds have activity in whole cells that reflect the potency and selectivity of target inhibition determined with this assay system. 相似文献
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Pectolytic Clostridium spp. in soils and rhizospheres of carrot and other arable crops in east Scotland 总被引:3,自引:3,他引:0
D.A. Perry 《Journal of applied microbiology》1982,52(3):403-408
Carrot root tissue discs rotted rapidly in anaerobic conditions after inoculation with soil from 40 different carrot fields and from ten fields of a mixed arable farm. Significant differences occurred in rotting potential between the soils and pectolytic Clostridium spp. were isolated from the rotted discs. Direct counts of pectolytic clostridia on selective pectate plates detected populations of 44–23.5 × 103 viable propagules/g dry soil in field soils. Significant increases in viable propagules were found in the rhizosphere soils of most carrot crops and in a range of other arable crops examined. The ratios of populations in the rhizospheres and surrounding soils varied between 1.8 and 8.2. Irrigating to excess and inter-row cultivation of carrot crops did not consistently affect populations in soil or on roots. 相似文献
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G. Pellegrino D. Caimi M. E. Noce A. Musacchio 《Plant Systematics and Evolution》2005,251(2-4):119-129
The rewardless orchid Dactylorhiza sambucina shows a stable flower colour polymorphism, with both yellow- and red-flowered morphs growing sympatrically. Pollination biology and breeding system were investigated to examine the effects of density of plants, colour polymorphism, inflorescence dimension, and flower position within inflorescence on male and female reproductive success in three natural populations of D. sambucina. There were significant differences among sites in the number of pollinia removed and in fruit set per inflorescence. Number of removed pollinia and capsule production in D. sambucina were independent from flower and inflorescence size or flower position. As a whole, the red morphs showed the highest number of capsules produced, while the yellow morphs had the greatest male success. The relative male and female reproductive success were independent from plant density but were significantly correlated with the yellow morph frequency at the population level. Overall, our findings show that the contribution to the total reproductive success deriving from the two colour morphs does not conform with the predictions of negative frequency-dependent selection. 相似文献
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