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Olga Maslova Oleg Fedevych Nadiia Shuvalova Olena Deryabina Volodymyr Zhovnir Miroslav Novak Peter Kruzliak 《Cell and tissue banking》2016,17(2):335-339
The need for selection of the optimal material for the manufacturing of cardio-patches can be resolved by the use of cryostored autologous pericardial tissue. This short communication is a concise fragment of a large-scale research and demonstrates only the efficiency of cell culturing before and after pericardial preservation in the low temperature conditions. 相似文献
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Marker-Dependent Recombination in T4 Bacteriophage. II. the Evaluation of Mismatch Repairabilities in Crosses within Indicator Distances 总被引:2,自引:1,他引:1 下载免费PDF全文
V. P. Shcherbakov L. A. Plugina E. A. Kudryashova O. I. Efremova S. T. Sizova Oleg G. Toompuu 《Genetics》1982,102(4):627-637
The contribution of mismatch repair to genetic recombination in T4 phage has been evaluated by three independent approaches: (1) testing for non-additivity of recombinant frequencies; (2) measurements of double exchange frequencies in three-factor crosses: (3) comparisons of recombination abilities of mutations occupying the same site. Quantitative agreement among the results of these approaches suggests that within distances much less than the mean length of hybrid regions, mismatch repair accounts perfectly for high negative interference as measured in three-factor crosses and as manifested by non-additivity in two-factor crosses. The mismatch repair mechanism readily recognizes only particular mismatches, the repair frequency being dependent on the base sequence in both strands of the mismatched region. 相似文献
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L. S. Mel’nikova I. V. Krivega P. G. Georgiev A. K. Golovnin 《Doklady biological sciences》2007,415(1):313-316
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Ricardo Gonzalez-Mendez David Wemmer George Hahn Norma Wade-Jardetzky Oleg Jardetzky 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1982,720(3):274-280
A continuous-flow NMR culture system for mammalian cells has been developed on which 31P-NMR experiments under complete and strictly physiologic conditions have been performed. Observations on the response of the cellular metabolism to stresses such as starvation, low temperature and changes in environmental pH monitored by 31P-NMR are reported. The response of the intracellular pH relative to the external pH of the growth medium is studied. We find that under the experimental conditions used there exists a ΔpH varying between less than 0.2 and more than 0.6 pH units. These results are compatible with those obtained using other techniques. 相似文献
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Maria Kuppner Stephen Wilkinson Elizabeth Casson Oleg Eremin 《Cancer immunology, immunotherapy : CII》1987,25(3):209-214
Summary Purified tumour cells and normal mucosa cells from fresh human colorectal cancer resection specimens, and T-cell-enriched autologous peripheral blood lymphocytes, were mixed in short-term (6 day) mixed lymphocytetumour cell (MLTC) microcultures. Lymphocyte stimulation was measured by 3H-thymidine uptake, and a stimulation index (SI=[lymphocytes vs tumour cells (cpm)–tumour cells (cpm)]/[lymphocytes (cpm)])>3 was regarded as significant. Significant lymphocyte reactivity was found in 10/15 patients with colon carcinoma. However, 1 patient with autologous tumour reactivity, also showed significant stimulation against autologous normal mucosa cells, suggesting tumour-associated reactivity. Maximum stimulation occurred most frequently at a lymphocyte:tumour cell ratio of 2:1 and with nylon wool-passaged lymphocytes.Project was supported by a grant from the Cancer Research CampaignSupported by New South Wales Cancer Council Fellowship 相似文献