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排序方式: 共有104条查询结果,搜索用时 78 毫秒
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A total of 55 mycoplasma strains, isolated from the vagina of goats, were examined. Three strains, being arginine positive and glucose negative, could not be finally classified. Five isolates were identified as Acholeplasma laidlawii. Fourty-seven strains were phosphatase positive, glucose and arginine negative. Nine of these formed “film and spots” on standard growth medium, and reduced tetrazolium aerobically. Serological examination identified 7 as M. agalactiae, while 2 were M. bovis. The remaining 38 isolates did not reduce tetrazolium aerobically, and did not produce “film and spots.” on standard growth medium. All these except one were identified as M. bovigenitalium by immunofluorescence. Their relationship to group 11 otf Al-Aubaidi is discussed. 相似文献
3.
Oluwole A. F. Ojo J. O. Durosinmi M. A. Asubiojo O. I. Akanle O. A. Spyrou N. M. Filby R. H. 《Biological trace element research》1994,43(1):443-452
Biological Trace Element Research - In this article, we present the elemental concentrations determined by INAA for 30 elements measured in some or all head hair samples of 100 Nigerian subjects... 相似文献
4.
Durosinmi M. A. Ojo J. O. Oluwole A. F. Ononye A. F. Akanle O. A. Spyrou N. M. 《Biological trace element research》1994,(1):357-361
The concentrations of a number of elements are determined in the plasma and erythrocytes of 21 Nigerians (11 females, 10 males)
with symptomatic affective disorders (11 depressives, 10 manics) and in 40 normal controls using proton-induced X-ray emission
(PIXE) analysis. The study shows that there is significant elevation of plasma K and Zn, as well as the erythrocyte S in the
patients relative to the controls. The plasma and erythrocyte Cu, and the erythrocyte P, Ca, Fe, and Zn are significantly
lower in the patients compared to the controls. However, the plasma levels of Ca, S, Fe, and Br are similar in both the patients
and the controls. Similarly, the concentrations of K, Br, and Rb show no significant difference in the erythrocytes of patients
and controls. 相似文献
5.
Study of trace elements in blood of cancer patients by proton-induced X-ray emission (PIXE) analysis
Durosinmi M. A. Ojo J. O. Oluwole A. F. Akanle O. A. Spyrou N. M. 《Biological trace element research》1994,(1):351-355
Proton-induced X-ray emission (PIXE) analysis was employed to determine the concentrations of six elements in the plasma and
erythrocytes of 18 cancer subjects (15 males and 3 females) with neoplastic disorders and in 70 controls (35 males and 35
females). It was found that the concentrations of Br, K, and Zn were significantly elevated in the erythrocytes of the cancer
subjects compared to the controls, whereas the concentration of Fe was significantly depressed, but with no difference observed
in the concentration of Ca. In the plasma, the concentrations of Br, Cu, Ca, and K were significantly elevated, whereas the
concentrations of Fe and Zn were found to be significantly depressed compared to the controls. 相似文献
6.
Kayode K. Ojo Ranae M. Ranade Zhongsheng Zhang David M. Dranow Janette B. Myers Ryan Choi Steve Nakazawa Hewitt Thomas E. Edwards Douglas R. Davies Donald Lorimer Stephen M. Boyle Lynn K. Barrett Frederick S. Buckner Erkang Fan Wesley C. Van Voorhis 《PloS one》2016,11(8)
We investigated Brucella melitensis methionyl-tRNA-synthetase (BmMetRS) with molecular, structural and phenotypic methods to learn if BmMetRS is a promising target for brucellosis drug development. Recombinant BmMetRS was expressed, purified from wild type Brucella melitensis biovar Abortus 2308 strain ATCC/CRP #DD-156 and screened by a thermal melt assay against a focused library of one hundred previously classified methionyl-tRNA-synthetase inhibitors of the blood stage form of Trypanosoma brucei. Three compounds showed appreciable shift of denaturation temperature and were selected for further studies on inhibition of the recombinant enzyme activity and cell viability against wild type B. melitensis strain 16M. BmMetRS protein complexed with these three inhibitors resolved into three-dimensional crystal structures and was analyzed. All three selected methionyl-tRNA-synthetase compounds inhibit recombinant BmMetRS enzymatic functions in an aminoacylation assay at varying concentrations. Furthermore, growth inhibition of B. melitensis strain 16M by the compounds was shown. Inhibitor-BmMetRS crystal structure models were used to illustrate the molecular basis of the enzyme inhibition. Our current data suggests that BmMetRS is a promising target for brucellosis drug development. However, further studies are needed to optimize lead compound potency, efficacy and safety as well as determine the pharmacokinetics, optimal dosage, and duration for effective treatment. 相似文献
7.
Gregory J. Crowther Heidi K. Hillesland Katelyn R. Keyloun Molly C. Reid Maria Jose Lafuente-Monasterio Sonja Ghidelli-Disse Stephen E. Leonard Panqing He Jackson C. Jones Mallory M. Krahn Jack S. Mo Kartheek S. Dasari Anna M. W. Fox Markus Boesche Majida El Bakkouri Kasey L. Rivas Didier Leroy Raymond Hui Gerard Drewes Dustin J. Maly Wesley C. Van Voorhis Kayode K. Ojo 《PloS one》2016,11(3)
In 2010 the identities of thousands of anti-Plasmodium compounds were released publicly to facilitate malaria drug development. Understanding these compounds’ mechanisms of action—i.e., the specific molecular targets by which they kill the parasite—would further facilitate the drug development process. Given that kinases are promising anti-malaria targets, we screened ~14,000 cell-active compounds for activity against five different protein kinases. Collections of cell-active compounds from GlaxoSmithKline (the ~13,000-compound Tres Cantos Antimalarial Set, or TCAMS), St. Jude Children’s Research Hospital (260 compounds), and the Medicines for Malaria Venture (the 400-compound Malaria Box) were screened in biochemical assays of Plasmodium falciparum calcium-dependent protein kinases 1 and 4 (CDPK1 and CDPK4), mitogen-associated protein kinase 2 (MAPK2/MAP2), protein kinase 6 (PK6), and protein kinase 7 (PK7). Novel potent inhibitors (IC50 < 1 μM) were discovered for three of the kinases: CDPK1, CDPK4, and PK6. The PK6 inhibitors are the most potent yet discovered for this enzyme and deserve further scrutiny. Additionally, kinome-wide competition assays revealed a compound that inhibits CDPK4 with few effects on ~150 human kinases, and several related compounds that inhibit CDPK1 and CDPK4 yet have limited cytotoxicity to human (HepG2) cells. Our data suggest that inhibiting multiple Plasmodium kinase targets without harming human cells is challenging but feasible. 相似文献
8.
Omotayo Sarafadeen Amuda Olumuyiwa Idowu Ojo Theresa Ibibia Edewor 《Bioremediation Journal》2007,11(4):183-194
The shell of the seed of Chrysophyllum albidum carbon was used to adsorb lead (Pb) from aqueous solution, the sorption process with respect to its equilibria and kinetics as well as the effects of pH, contact time, adsorbent mass, adsorbate concentration, and particle size on adsorption were also studied. The most effective pH range was found to be between 4.5 and 5 for the sorption of the metal ion. The first-order rate equation by Lagergren was tested on the kinetic data and the adsorption process followed first-order rate kinetics. Isotherm data were analyzed for possible agreement with the Langmuir and Freundlich adsorption isotherms; the Freundlich and Langmuir models for dynamics of metal ion uptake proposed in this work fitted the experimental data reasonably well. However, equilibrium sorption data were better represented by Langmuir model than Freundlich. The adsorption capacity calculated from Langmuir isotherm was 72.1 mg Pb (II) g- 1 at initial pH of 5.0 at 30°C for the particle size of 1.00 to 1.25 mm with the use of 2.0 g/100 ml adsorbent mass. The structural features of the adsorbent were characterized by Fourier transform infrared (FTIR) spectrometry; the presence of hydroxyl, carbonyl, amide, and phosphate groups confirms the potential mechanism adsorption of the adsorbent. This readily available adsorbent is efficient in the uptake of Pb (II) ion in aqueous solution, thus, it could be an excellent alternative for the removal of heavy metals and organic matter from water and wastewater. 相似文献
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10.
Ponnada Suresh Kumar KK Pulicherla Mrinmoy Ghosh Anmol Kumar KRS Sambasiva Rao 《Bioinformation》2011,6(8):311-314
Enzymes from psychrophiles catalyze the reactions at low temperatures with higher specific activity. Among all the psychrophilic enzymes produced, cold active
β-galactosidase from marine psychrophiles revalorizes a new arena in numerous areas at industrial level. The hydrolysis of lactose in to glucose and galactose by
cold active β-galactosidase offers a new promising approach in removal of lactose from milk to overcome the problem of lactose intolerance. Herein we propose, a
3D structure of cold active β-galactosidase enzyme sourced from Pseudoalteromonas haloplanktis by using Modeler 9v8 and best model was developed having
88% of favourable region in ramachandran plot. Modelling was followed by docking studies with the help of Auto dock 4.0 against the three substrates lactose,
ONPG and PNPG. In addition, comparative docking studies were also performed for the 3D model of psychrophilic β-galactosidase with mesophilic and
thermophilic enzymes. Docking studies revealed that binding affinity of enzyme towards the three different substrates is more for psychrophilic enzyme when
compared with mesophilic and thermophilic enzymes. It indicates that the enzyme has high specific activity at low temperature when compared with mesophilic
and thermophilic enzymes. 相似文献