全文获取类型
收费全文 | 2298篇 |
免费 | 103篇 |
国内免费 | 3篇 |
出版年
2024年 | 2篇 |
2023年 | 33篇 |
2022年 | 34篇 |
2021年 | 143篇 |
2020年 | 87篇 |
2019年 | 98篇 |
2018年 | 124篇 |
2017年 | 103篇 |
2016年 | 135篇 |
2015年 | 180篇 |
2014年 | 170篇 |
2013年 | 230篇 |
2012年 | 216篇 |
2011年 | 185篇 |
2010年 | 99篇 |
2009年 | 78篇 |
2008年 | 66篇 |
2007年 | 73篇 |
2006年 | 50篇 |
2005年 | 55篇 |
2004年 | 39篇 |
2003年 | 35篇 |
2002年 | 27篇 |
2000年 | 11篇 |
1999年 | 7篇 |
1998年 | 7篇 |
1997年 | 8篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1994年 | 6篇 |
1993年 | 5篇 |
1992年 | 7篇 |
1991年 | 9篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1985年 | 4篇 |
1984年 | 10篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1970年 | 1篇 |
1966年 | 1篇 |
1965年 | 2篇 |
1964年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有2404条查询结果,搜索用时 15 毫秒
1.
2.
3.
Azemi Nur Farah Hani Misnan Rosmilah Keong Bun Poh Mokhtar Marina Kamaruddin Nurhaida Fah Wong Chee Yadzir Zailatul Hani Mohamad Yadzir Hani Mohamad Bakhtiar Faizal Abdullah Noormalin Arip Masita Ateshan Haidr Msahir 《Molecular biology reports》2021,48(10):6709-6718
Molecular Biology Reports - Tropomyosin is a major allergen in crustaceans, including mud crab species, but its molecular and allergenic properties in Scylla olivacea are not well known. Thus, this... 相似文献
4.
Isolation and screening of potential actinobacteria for rapid composting of rice straw 总被引:2,自引:0,他引:2
Rice straw is produced as a by-product from rice cultivation, which is composed largely of lignocellulosic materials amenable
to general biodegradation. Lignocellulolytic actinobacteria can be used as a potential agent for rapid composting of bulky
rice straw. Twenty-five actinobacteria isolates were isolated from various in situ and in vitro rice straw compost sources.
Isolates A2, A4, A7, A9 and A24 were selected through enzymatic degradation of starch, cellulose and lignin followed by the
screening for their adaptability on rice straw powder amended media. The best adapted isolate (A7) was identified as Micromonospora
carbonacea. It was able to degrade cellulose, hemicelluloses and carbon significantly (P ≤ 0.05) over the control. C/N ratio was reduced to 18.1 from an initial value of 29.3 in 6 weeks of composting thus having
the potential to be used in large scale composting of rice straw. 相似文献
5.
Hasidah Mohd. Sidek Cynthia Nyquist-Battie Garret Vanderkooi 《Biochimica et Biophysica Acta (BBA)/General Subjects》1984,801(1):26-31
The effects of tertiary amine local anesthetics (procaine, lidocaine, tetracaine and dibucaine) and chlorpromazine were investigated for three enzyme activities associated with rat brain synaptosomal membranes, i.e., (Na+ + K+)-ATPase (ouabain-sensitive), Mg2+-ATPase (ouabain-insensitive) and acetylcholinesterase. Approximately the same concentrations of each agent gave 50% inhibition of both ATPase, for example 7.9 and 10 mM tetracaine for Mg2+-ATPase and (Na+ + K+)-ATPase, respectively; these concentrations are 10-fold higher than required for inhibition of mitochondrial F1-ATPase. The relative inhibitory potency of the several agents was proportional to their octanol/water partition coefficients. Acetylcholinesterase was inhibited by all agents tested, but the ester anesthetics (procaine and tetracaine) were considerably more potent than the others after correction for partition coefficient differences. For tetracaine, 0.18 mM gave 50% inhibition and showed competitive inhibition on a Lineweaver-Burk plot, but for dibucaine a mixed type of inhibition was observed, and 0.63 mM was required for 50% inhibition. Tetracaine evidently binds at the active site, and dibucaine at the peripheral or modulator site, on this enzyme. 相似文献
6.
7.
8.
In some circumstances, like driving a motor vehicle, group membership is irrelevant. In others, individual interest motivates group alignment. Sometimes choices have to be made between individual responses and group alignment, or between alignment on the basis of class, or ethnicity, or religion. The authors describe a technique used to assess the strength of ethnic loyalty relative to self‐interest and personal obligation among an urban sample in Malaysia. Predictions of how a representative Malay would react in situations of value conflict made possible the measurement of pluralistic ignorance. The technique could be used elsewhere. It could be used to generate predictions about trends in the strengthening, maintenance or weakening of ethnic boundaries. 相似文献
9.
10.
Azzmer Azzar Abdul Hamid Tengku Haziyamin Tengku Abdul Hamid Roswanira Abdul Wahab Mohd. Shahir Shamsir Omar Fahrul Huyop 《PloS one》2015,10(3)
The non-stereospecific α-haloalkanoic acid dehalogenase E (DehE) degrades many halogenated compounds but is ineffective against β-halogenated compounds such as 3-chloropropionic acid (3CP). Using molecular dynamics (MD) simulations and site-directed mutagenesis we show here that introducing the mutation S188V into DehE improves substrate specificity towards 3CP. MD simulations showed that residues W34, F37, and S188 of DehE were crucial for substrate binding. DehE showed strong binding ability for D-2-chloropropionic acid (D-2CP) and L-2-chloropropionic acid (L-2CP) but less affinity for 3CP. This reduced affinity was attributed to weak hydrogen bonding between 3CP and residue S188, as the carboxylate of 3CP forms rapidly interconverting hydrogen bonds with the backbone amide and side chain hydroxyl group of S188. By replacing S188 with a valine residue, we reduced the inter-molecular distance and stabilised bonding of the carboxylate of 3CP to hydrogens of the substrate-binding residues. Therefore, the S188V can act on 3CP, although its affinity is less strong than for D-2CP and L-2CP as assessed by Km. This successful alteration of DehE substrate specificity may promote the application of protein engineering strategies to other dehalogenases, thereby generating valuable tools for future bioremediation technologies. 相似文献