全文获取类型
收费全文 | 100211篇 |
免费 | 9111篇 |
国内免费 | 2383篇 |
出版年
2023年 | 489篇 |
2022年 | 524篇 |
2021年 | 2318篇 |
2020年 | 1490篇 |
2019年 | 1886篇 |
2018年 | 2112篇 |
2017年 | 1740篇 |
2016年 | 2780篇 |
2015年 | 4696篇 |
2014年 | 5263篇 |
2013年 | 6081篇 |
2012年 | 8079篇 |
2011年 | 7852篇 |
2010年 | 5149篇 |
2009年 | 4690篇 |
2008年 | 6419篇 |
2007年 | 6289篇 |
2006年 | 6012篇 |
2005年 | 5666篇 |
2004年 | 5439篇 |
2003年 | 5164篇 |
2002年 | 4831篇 |
2001年 | 1073篇 |
2000年 | 811篇 |
1999年 | 1128篇 |
1998年 | 1368篇 |
1997年 | 907篇 |
1996年 | 741篇 |
1995年 | 702篇 |
1994年 | 693篇 |
1993年 | 704篇 |
1992年 | 593篇 |
1991年 | 542篇 |
1990年 | 479篇 |
1989年 | 411篇 |
1988年 | 428篇 |
1987年 | 364篇 |
1986年 | 327篇 |
1985年 | 409篇 |
1984年 | 522篇 |
1983年 | 410篇 |
1982年 | 507篇 |
1981年 | 492篇 |
1980年 | 415篇 |
1979年 | 300篇 |
1978年 | 321篇 |
1977年 | 281篇 |
1976年 | 264篇 |
1975年 | 203篇 |
1974年 | 242篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
1.
The chi-square periodogram (CSP), developed over 40 years ago, continues to be one of the most popular methods to estimate the period of circadian (circa 24-h) rhythms. Previous work has indicated the CSP is sometimes less accurate than other methods, but understanding of why and under what conditions remains incomplete. Using simulated rhythmic time-courses, we found that the CSP is prone to underestimating the period in a manner that depends on the true period and the length of the time-course. This underestimation bias is most severe in short time-courses (e.g., 3 days), but is also visible in longer simulated time-courses (e.g., 12 days) and in experimental time-courses of mouse wheel-running and ex vivo bioluminescence. We traced the source of the bias to discontinuities in the periodogram that are related to the number of time-points the CSP uses to calculate the observed variance for a given test period. By revising the calculation to avoid discontinuities, we developed a new version, the greedy CSP, that shows reduced bias and improved accuracy. Nonetheless, even the greedy CSP tended to be less accurate on our simulated time-courses than an alternative method, namely the Lomb-Scargle periodogram. Thus, although our study describes a major improvement to a classic method, it also suggests that users should generally avoid the CSP when estimating the period of biological rhythms. 相似文献
2.
The effects of 5-methoxy-N, N-dimethyltryptamine (5-MeODMT), a serotonin agonist with a preferential action on presynaptic autoreceptors, on prolactin release in male rats was determined. Basal serum prolactin levels were not altered after administration of 1.0, 2.0, 5.0, 10.0 or 20.0 mg/kg of 5-MeODMT.Pretreatment with 5-MeODMT reduced prolactin release by agents that depend on serotonergic neurotransmission for part of their prolactin release stimulation. Prolactin release in response to L-5-hydroxytryptophan (5-HTP) or morphine was significantly reduced by pretreatment of the rats with 5-MeODMT.The results of this experiment indicate that 5-MeODMT act as a presynaptic serotonin autoreceptor stimulant and not as a postsynaptic serotonin agonist on the neuronal systems that control prolactin release. 相似文献
3.
Plant somatic cells have the capability to switch their cell fates from differentiated to undifferentiated status under proper
culture conditions, which is designated as totipotency. As a result, plant cells can easily regenerate new tissues or organs
from a wide variety of explants. However, the mechanism by which plant cells have such remarkable regeneration ability is
still largely unknown. In this study, we used a set of meristem-specific marker genes to analyze the patterns of stem cell
differentiation in the processes of somatic embryogenesis as well as shoot or root organogenesis in vitro. Our studies furnish preliminary and important information on the patterns of the de novo stem cell differentiation during various types of in vitro organogenesis. 相似文献
4.
Vinogradov Michael E.; Shushkina Elvira A.; Nezlin Nikolay P.; Arnautov Genrikh N. 《Journal of plankton research》1998,20(1):85-103
Zooplankton data collected during September 1995 in the NorthWest Atlantic at 4139'N, 4958'W (the location of the siteof the Titanic wreck) were analysed. The regioninvestigated was characterized by a very sharp frontal zonebetween the Gulf Stream and the main stream of the LabradorCurrent. The total plankton biomass in the water column wasvery high. The macroplankton biomass values below the 600 mlayer were significantly higher as compared with the similarvalues measured before in other productive boreal regions ofthe Atlantic and Pacific oceans. A lot of dead mesoplanktonanimals occurred in the deep layers. The reason was that thecold-water mesoplankton advected by the Labrador Current diedoff intensively within the deep layers of the frontal zone andwere used as a food resource by the macroplankton carnivoresand scavengers that were very abundant there. 相似文献
5.
Michael Marmot 《CMAJ》2012,184(11):1231-1232
6.
7.
8.
9.
10.
Ting‐Hang Liu Chia‐Lin Chyan Feng‐Yin Li Ying‐Jie Chen Jason T. C. Tzen 《Biotechnology progress》2011,27(6):1760-1767
It has been demonstrated that caleosin alone is sufficient to stabilize artificial oil bodies. A series of recombinant caleosins, mutated with 3, 5, 8, 11, 13, 15, and 17 extra Lys residues and over‐expressed in Escherichia coli, were used as carrier proteins to render biotin as a hapten on the surface of artificial oil bodies for antibody production. Biotinylation levels of the recombinant caleosins were step‐wisely elevated as the number of extra Lys residues increased, and the biotinylated Lys residues were identified by mass spectrometric analysis. Polyclonal antibodies against biotin were successfully generated in rats injected with artificial oil bodies constituted with each of the biotinylated caleosins. Moreover, those generated via the biotinylated caleosins with eight or more extra Lys residues no longer recognized caleosin. It appears that engineered Lys‐rich caleosins are suitable carrier proteins for the production of antibodies against small molecules. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011 相似文献