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Methyl mercury uptake in free cells and different immobilizates of the cyanobacteriumNostoc calcicola has been examined. The general growth of the immobilized cyanobacterial cells could be negatively correlated with methyl mercury uptake. Alginate spheres proved most efficient in terms of uptake rate (0.48 nmol mg protein–1 min–1, 10 min) and total bioaccumulation (10.71 nmol mg protein–1, 1 h) with a bioconcentration factor of 3.3×103. Alginate biofilms showed a faster methyl mercury accumulation rate (0.83 nmol mg protein–1 min–1, 10 min) with a saturation of 10.28 nmol mg protein–1 reached within only 30 min (bioconcentration factor, 3.1×103). Foam preparations with a slow initial uptake approximated biofilms but were characterized by a lower bioconcentration factor (2.8×103). Free cells, in comparison, maintained the initial slow rate of uptake (0.62 nmol mg protein–1 min–1, 10 min), saturating at 30 min (8.81 nmol mg protein–1), and the resultant lowest bioconcentration factor (2.7×103). Cell ageing (30 days) brought a drastic reduction (3-fold) in organomercury uptake by free cells while alginate spheres maintained the same potential. Foam preparations of the same age showed a significant improvement in methyl mercury uptake followed by only a marginal decline in alginate biofilms. Data are discussed in the light of the physiological efficiency and longevity of immobilized cells. 相似文献
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Calcium-Dependent 4-aminopyridine stimulation of protein phosphorylation in squid optic lobe synaptosomes 总被引:1,自引:0,他引:1
Harish C. Pant Paul E. Gallant Rochelle Cohen Joseph T. Neary Harold Gainer 《Cellular and molecular neurobiology》1983,3(3):223-238
When intact synaptosomes were incubated with [gamma-32P]ATP, maximal protein phosphorylation was attained 2 min after the start of incubation. Protein phosphorylation under basal conditions was dependent on external Ca2+, and the dominant peak of phosphorylation was a 50-kd protein. Incubation of intact synaptosomes in the presence of 3-6 mM 4-aminopyridine (4-AP) caused a markedly enhanced phosphorylation of high molecular weight proteins of 90, 100, 130, and 180 kd, with no increase in the 50 or 38 kd proteins. This effect of 4-AP was dependent on external calcium ions in the incubation medium. The 4-AP effect on the high molecular weight proteins was also found in synaptosomal plasma membranes isolated from the synaptosomes. Tetraethylammonium (TEA) ions did not produce this enhancement of phosphorylation. 相似文献
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Thirty-six cases of solitary and scintigraphically "cold" thyroid nodules were studied by fine needle aspiration (FNA) cytology, ultrasonography, radionuclide perfusion study (RPS) and xeroradiography with the aim of differentiating the neoplastic from the nonneoplastic nodules. Histologic study of the excised specimens provided the definitive diagnosis in all cases. Of the techniques used in this study, FNA cytology and RPS had the highest sensitivities and specificities. Ultrasonography and xeroradiography were of limited use due to their low sensitivity rates. 相似文献
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Summary The susceptibility of 50 drug resistant strains of Escherichia coli of human gut was determined against ciprofloxacin, acridine orange (AO) and sodium dodecyl sulphate (SDS). Curing efficacy of these agents were worked out at subminimal inhibitory concentrations. Ciprofloaxacin was found a better curing agent for E coli R-plasmids, eliminating R-factors from 48% of the strains followed by SDS and AO which eliminated 24% and 20% of the drug resistance determinants, respectively. Elimination of R-plasmids was found dependent on the concentrations of curing agents and nature of R-plasmids. 相似文献
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The effect of population size, redox potential, exogenous ATP and complexing agents on Cu uptake by free and immobilized cyanobacteriumNostoc calcicola
Bréb. has been studied. Cu uptake was regulated by the population size. In such comparisons, the immobilized cells had a greater
longevity. Low pH conditions enhanced Cu uptake. Exogenous ATP (10 μmol/L) supplied to dark-grown free and immobilized cells
did not support Cu uptake to the extent of light-grown cells. Experiments involving natural as well as synthetic complexing
agents clearly established the superiority of soil extract and spent medium over EDTA (10 μmol/L), in sequestering Cu in free
as well as immobilized cells. 相似文献
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Changes in carbohydrate and lipid metabolism during embryonic development inAntheraea mylitta were studied. While carbohydrates were metabolized during early embryogenesis, lipids were catabolised at the later stages.
A significant increase in both total carbohydrates and glycogen on days 5 and 6 suggested the concurrent occurrence of both
gluconeogenesis and glycogenesis. As the development of the embryo proceeds, both lipids and carbohydrates were utilised,
resulting in the increase in the concentration of citrate, pyruvate and lactate. 相似文献