The identification of the membrane proteins of human and animal viruses]

The review deals with the methods of identification of virus-specific proteins on virion and infected cell surface. The isotopic labeling of membrane proteins, their extraction by proteolytic enzymes and selective solubilization by detergents are considered. The plasmatic membrane isolation by centrifugation and by means of microcarriers is described. The methods of membrane protein localization using monoclonal antibodies and radioimmunoprecipitation are presented.     

External control of the Drosophila melanogaster lifespan by combination of 3D oscillating low-frequency electric and magnetic fields

We demonstrate that the lifespan of Drosophila melanogaster population is controllable by a combination of external three-dimensional oscillating low-frequency electric and magnetic fields (3D OLFEMFs). The lifespan was decreased or increased in dependence of the parameters of the external 3D OLFEMFs. We propose that metabolic processes in D. melanogaster’s body are either accelerated (in the case of reduced lifespan) or slowed down (in the case of increased lifespan) in function of 3D OLFEMFs that induce vibrational motions on sub-cellular and larger scales.     

Anti-amyloid Therapy of Alzheimer’s Disease: Current State and Prospects

Drug development for the treatment of Alzheimer’s disease (AD) has been for a long time focused on agents that were expected to support endogenous β-amyloid (Aβ) in a monomeric state and destroy soluble Aβ oligomers and insoluble Aβ aggregates. However, this strategy has failed over the last 20 years and was eventually abandoned. In this review, we propose a new approach to the anti-amyloid AD therapy based on the latest achievements in understanding molecular causes of cerebral amyloidosis in AD animal models.     

Development and structure of epithelial formations of the connective tissue stroma of human gingiva

Processes of development and structure of epithelial formations, localized in the connective tissue framework of the gingival mucous membrane have been studied. During the prenatal period of development they are presented as epithelial pearls of Serra, and after birth--as remnants of ++tooth-forming epithelium (RTE). They are remnants of sheaths of the destroyed pearls. The latter, like RTE in the gingival stroma, have a genetic connection with the epithelial islets of Malasser and can cause some diseases in the gingiva.     

Immunoenzyme analysis of ovarian metastatic antigen-8]

Immunoenzymatic method for the determination of new ovarian-metastatic antigen-8 in human blood serum was worked out. OMA-8 level was studied in blood serum of 40 healthy women, 40 healthy men, 16 neonates, 33 pregnant women, 103 patients with genital tumours. It was found out that OMA-8 level in blood serum of healthy women changed between 2 to 15.4 micrograms/l, in men between 5-17.0 microgramS/l. OMA-8 concentration higher than 15.4 micrograms/ was considered elevated. The elevated OMA-8 level was marked in neonates (100%) and in pregnant (39.3%). High level was discovered in the amniotic fluid.     

The chromatin fiber: structure and conformational transitions as revealed by optical anisotropy studies

Structure and conformational transitions of the chromatin fiber as revealed by optical anisotropy studies are reviewed. The data in the literature do not allow a definite interpretation; in fact some of them are contradictory. The major findings are reported here and an attempt is made to analyse them with respect to the internal dynamics and the various models suggested for the organization of the chromatin fiber.