全文获取类型
收费全文 | 595篇 |
免费 | 59篇 |
国内免费 | 1篇 |
出版年
2021年 | 8篇 |
2018年 | 5篇 |
2017年 | 5篇 |
2016年 | 12篇 |
2015年 | 19篇 |
2014年 | 24篇 |
2013年 | 21篇 |
2012年 | 30篇 |
2011年 | 21篇 |
2010年 | 25篇 |
2009年 | 14篇 |
2008年 | 24篇 |
2007年 | 21篇 |
2006年 | 24篇 |
2005年 | 20篇 |
2004年 | 22篇 |
2003年 | 19篇 |
2002年 | 17篇 |
2001年 | 9篇 |
2000年 | 23篇 |
1999年 | 8篇 |
1998年 | 9篇 |
1997年 | 6篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1994年 | 6篇 |
1993年 | 5篇 |
1992年 | 10篇 |
1991年 | 15篇 |
1990年 | 17篇 |
1989年 | 13篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 7篇 |
1980年 | 8篇 |
1979年 | 11篇 |
1975年 | 8篇 |
1974年 | 6篇 |
1971年 | 4篇 |
1969年 | 5篇 |
1968年 | 5篇 |
1958年 | 8篇 |
1957年 | 4篇 |
1956年 | 4篇 |
1954年 | 6篇 |
1953年 | 5篇 |
1951年 | 9篇 |
1949年 | 4篇 |
排序方式: 共有655条查询结果,搜索用时 31 毫秒
1.
2.
3.
4.
5.
6.
7.
8.
Daiane D. Hartwig Kátia L. Bacelo Patrícia D. de Oliveira Thaís L. Oliveira Fabiana K. Seixas Marta G. Amaral Cláudia P. Hartleben Alan J. A. McBride Odir A. Dellagostin 《Current microbiology》2014,68(4):524-530
The C-terminal region of the Leptospiral immunoglobulin-like A protein (LigA) contains six carboxy-terminal Ig-like repeat domains (LigANI). Subunit vaccine preparations based on recombinant LigANI produced in Escherichia coli, are promising vaccine candidates, albeit with variable efficacy. In the present study, LigANI was expressed in the methylotrophic yeast Pichia pastoris using a 12 L bioreactor to produce mannosylated LigANI (mLigANI) for use in a vaccine preparation against leptospirosis. Hamsters immunized with a mLigANI vaccine preparation produced a significant IgG antibody response (P < 0.001) and were protected (83.3 %; P < 0.001) against lethal challenge with 36× LD50 of a virulent strain of L. interrogans serovar Copenhageni. A vaccine preparation based on demannosylated mLigANI (nmLigANI) elicited an immune response in hamsters, but did not afford protection. The production of mLigANI in bioreactor by P. pastoris yielded ~50 mg L?1 of recombinant protein. P. pastoris is a potential platform for the production of leptospiral antigens on an industrial scale. The results demonstrate that LigANI secreted by P. pastoris on mannosylated form (mLigANI) protect hamsters as subunit vaccine of L. interrogans lethal infection. 相似文献
9.
10.
Gerald Drews Kay Kohlhaw Daniel Palmes Petra Madaj-Sterba Thomas Hartwig Johann Hauss Hans-Ullrich Spiegel 《Journal of Experimental Animal Science》2000,41(3)
Sampling of rat hepatic tissue for histomorphological analysis is usually performed in two different ways: either by killing the animals or by minilaparotomy.In this study we describe a percutaneous core biopsy technique which has been used on day 7, 14 and 21 after allogeneic rat liver transplantation (DA → LEW) in order to examine grafts for rejection in different treatment groups. Fifty-two liver biopsies were performed in 24 animals using a 16-gauge intravenous cannula. Forty-five provided usable specimens which were sufficient for both light or electron microscopy and immunohistochemical analyses to determine the degree of graft rejection. In 7 cases (13.5%) sampling was unsuccessful, especially on day 21 after transplantation, as the plastic cannula could not penetrate the hardened tissue. In 3 animals (5.8%) puncture was immediately followed by death due to perforation of the diaphragm or ether intoxication.In conclusion, this technique is a reliable method for providing ample tissue samples from the rat liver with a low risk of complications. 相似文献