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排序方式: 共有118条查询结果,搜索用时 37 毫秒
1.
K W Culver W T Hsieh Y Huyen V Chen J Liu Y Khripine A Khorlin 《Nature biotechnology》1999,17(10):989-993
A sequence-specific genomic delivery system for the correction of chromosomal mutations was designed by incorporating two different binding domains into a single-stranded oligonucleotide. A repair domain (RD) contained the native sequence of the target region. A third strand-forming domain (TFD) was designed to form a triplex by Hoogsteen interactions. The design was based upon the premise that the RD will rapidly form a heteroduplex that is anchored synergistically by the TFD. Deoxyoligonucleotides were designed to form triplexes in the human adenosine deaminase (ADA) and p53 genes adjacent to known point mutations. Transfection of ADA-deficient human lymphocytes corrected the mutant sequence in 1-2% of cells. Neither the RD or TFD individually corrected the mutation. Transfection of p53 mutant human glioblastoma cells corrected the mutation and induced apoptosis in 7.5% of cells. 相似文献
2.
Viacheslav N. Kachalov Huyen Nguyen Suraj Balakrishna Luisa Salazar-Vizcaya Rami Sommerstein Stefan P. Kuster Anthony Hauser Pia Abel zur Wiesch Eili Klein Roger D. Kouyos 《PLoS computational biology》2021,17(1)
Beta-lactam- and in particular carbapenem-resistant Enterobacteriaceae represent a major public health threat. Despite strong variation of resistance across geographical settings, there is limited understanding of the underlying drivers. To assess these drivers, we developed a transmission model of cephalosporin- and carbapenem-resistant Klebsiella pneumoniae. The model is parameterized using antibiotic consumption and demographic data from eleven European countries and fitted to the resistance rates for Klebsiella pneumoniae for these settings. The impact of potential drivers of resistance is then assessed in counterfactual analyses. Based on reported consumption data, the model could simultaneously fit the prevalence of extended-spectrum beta-lactamase-producing and carbapenem-resistant Klebsiella pneumoniae (ESBL and CRK) across eleven European countries over eleven years. The fit could explain the large between-country variability of resistance in terms of consumption patterns and fitted differences in hospital transmission rates. Based on this fit, a counterfactual analysis found that reducing nosocomial transmission and antibiotic consumption in the hospital had the strongest impact on ESBL and CRK prevalence. Antibiotic consumption in the community also affected ESBL prevalence but its relative impact was weaker than inpatient consumption. Finally, we used the model to estimate a moderate fitness cost of CRK and ESBL at the population level. This work highlights the disproportionate role of antibiotic consumption in the hospital and of nosocomial transmission for resistance in gram-negative bacteria at a European level. This indicates that infection control and antibiotic stewardship measures should play a major role in limiting resistance even at the national or regional level. 相似文献
3.
Hui Wu Charles J. Kelley Alejandro Pino-Figueroa Huyen D. Vu Timothy J. Maher 《Bioorganic & medicinal chemistry》2013,21(17):5188-5197
Maca (Lepidium meyenii), a traditional food crop of the Peruvian Andes is now widely touted as a dietary supplement. Among the various chemical constituents isolated from the plant are a unique series of non-polar, long-chain fatty acid N-benzylamides known as macamides. We have synthesized 11 of the 19 reported macamides and have tested each as potential inhibitors of the human enzyme, fatty acid amide hydrolase (FAAH). The five most potent macamides were FAAH inhibitors (IC50 = 10–17 μM). These amides were derivatives of oleic, linoleic and linolenic acids and benzylamine or 3-methoxybenzylamine. Of the three compounds evaluated in a pre-incubation time study, two macamides were not irreversible inhibitors of FAAH. The third, a carbamate structurally related to macamides, was shown to be an irreversible inhibitor of FAAH (IC50 = 0.153 μM). 相似文献
4.
Andrew Wilby La Pham Lan Kong Luen Heong Nguyen Phi Dieu Huyen Nguyen Huu Quang Nguyen Viet Minh Matt. B Thomas 《Ecosystems》2006,9(4):538-549
Declining biodiversity in agro-ecosystems, caused by intensification of production or expansion of monocultures, is associated
with the emergence of agricultural pests. Understanding how land-use and management control crop-associated biodiversity is,
therefore, one of the key steps towards the prediction and maintenance of natural pest-control. Here we report on relationships
between land-use variables and arthropod community attributes (for example, species diversity, abundance and guild structure)
across a diversification gradient in a rice-dominated landscape in the Mekong delta, Vietnam. We show that rice habitats contained
the most diverse arthropod communities, compared with other uncultivated and cultivated land-use types. In addition, arthropod
species density and Simpson’s diversity in flower, vegetable and fruit habitats was positively related to rice cover in the
local landscape. However, across the landscape as a whole, reduction in heterogeneity and the amount of uncultivated cover
was associated, generally, with a loss of diversity. Furthermore, arthropod species density in tillering and flowering stages
of rice was positively related to crop and vegetation richness, respectively, in the local landscape. Differential effects
on feeding guilds were also observed in rice-associated communities with the proportional abundance of predators increasing
and the proportional abundance of detritivores decreasing with increased landscape rice cover. Thus, we identify a range of
rather complex, sometimes contradictory patterns concerning the impact of rice cover and landscape heterogeneity on arthropod
community attributes. Importantly, we conclude that that land-use change associated with expansion of monoculture rice need
not automatically impact diversity and functioning of the arthropod community. 相似文献
5.
Le Cong Huyen Bao Phan Tran Crepin Nathalie Rolland Filip Van Dijck Patrick 《Plant molecular biology》2022,108(6):531-547
Plant Molecular Biology - Alternative translation initiation of the unique Arabidopsis trehalase gene allows for the production of two isoforms with different subcellular localization, providing... 相似文献
6.
A functionally conserved Zn2Cys6 binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat 下载免费PDF全文
7.
Nguyen Van Tho Le Thi Huyen Trang Yoshitaka Murakami Emiko Ogawa Yasushi Ryujin Rie Kanda Hiroaki Nakagawa Kenichi Goto Kentaro Fukunaga Yuichi Higami Ruriko Seto Taishi Nagao Tetsuya Oguma Masafumi Yamaguchi Le Thi Tuyet Lan Yasutaka Nakano 《PloS one》2014,9(5)
Background
It is time-consuming to obtain the square root of airway wall area of the hypothetical airway with an internal perimeter of 10 mm (√Aaw at Pi10), a comparable index of airway dimensions in chronic obstructive pulmonary disease (COPD), from all airways of the whole lungs using 3-dimensional computed tomography (CT) analysis. We hypothesized that √Aaw at Pi10 differs among the five lung lobes and √Aaw at Pi10 derived from one certain lung lobe has a high level of agreement with that derived from the whole lungs in smokers.Methods
Pulmonary function tests and chest volumetric CTs were performed in 157 male smokers (102 COPD, 55 non-COPD). All visible bronchial segments from the 3rd to 5th generations were segmented and measured using commercially available 3-dimensional CT analysis software. √Aaw at Pi10 of each lung lobe was estimated from all measurable bronchial segments of that lobe.Results
Using a mixed-effects model, √Aaw at Pi10 differed significantly among the five lung lobes (R2 = 0.78, P<0.0001). The Bland-Altman plots show that √Aaw at Pi10 derived from the right or left upper lobe had a high level of agreement with that derived from the whole lungs, while √Aaw at Pi10 derived from the right or left lower lobe did not.Conclusion
In male smokers, CT-derived airway wall area differs among the five lung lobes, and airway wall area derived from the right or left upper lobe is representative of the whole lungs. 相似文献8.
MN Kaggwa N Straubinger-Gansberger M Schagerl 《African Journal of Aquatic Science》2018,43(2):123-129
Nine small artificial dams located in different climatic regions of Kenya were studied. The local communities use the stored water for various purposes, such as irrigation, domestic use, watering of livestock and cage fish farming. Such intense use is commonly accompanied by eutrophication, including fast growth of cyanobacteria, which at times produce cyanotoxins threatening human and animal life. We studied the pelagic community, analysed abiotic variables and identified microcystins by means of high performance liquid chromatography and ELISA kits at monthly intervals over a period of one year. Mass spectrometry (MALDI-TOF MS) was used to identify structural variants of microcystins by their protonated masses (M + H). Three dams contained microcystins, with the highly toxic Microcystin-LR being identified as the most prominent substance. Cell content of the toxin varied from 7.2 to 686.7 fg cell?1. Basic limnological variables that indicate the probability of toxin presence were also recorded. Non-parametric Mann–Whitney U-test revealed significant differences in soluble reactive phosphorous, nitrate-N, water depth, total hardness and post-Nauplii stages sampled between toxin-producing and non-toxin-producing dams. Although most of the samples did not contain high amounts of cyanobacteria, the cyanotoxin-problem was evident, suggesting the need for regular cyanotoxin monitoring programs. 相似文献
9.
10.
Heiman F. L. Wertheim Huyen Nguyen Nguyen Walter Taylor Trinh Thi Minh Lien Hoa Thi Ngo Thai Quoc Nguyen Bich Ngoc Thi Nguyen Ha Hong Nguyen Ha Minh Nguyen Cap Trung Nguyen Trinh Tuyet Dao Trung Vu Nguyen Annette Fox Jeremy Farrar Constance Schultsz Hien Duc Nguyen Kinh Van Nguyen Peter Horby 《PloS one》2009,4(6)