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A systematic analysis of the RNA splice junction sequences of eukaryotic protein coding genes was carried out using the GENBANK databank. Nucleotide frequencies obtained for the highly conserved regions around the splice sites for different categories of organisms closely agree with each other. A striking similarity among the rare splice junctions which do not contain AG at the 3' splice site or GT at the 5' splice site indicates the existence of special mechanisms to recognize them, and that these unique signals may be involved in crucial gene-regulation events and in differentiation. A method was developed to predict potential exons in a bare sequence, using a scoring and ranking scheme based on nucleotide weight tables. This method was used to find a majority of the exons in selected known genes, and also predicted potential new exons which may be used in alternative splicing situations.  相似文献
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Automated preparation of DNA sequences for publication.   总被引:9,自引:0,他引:9       下载免费PDF全文
A computer program which draws DNA sequences is described. A simple method is used which enables the user to highlight or annotate specific parts of a sequence. The sizes of the characters in the sequence to be drawn are specified by the user. In addition, vertical spacing between lines and horizontal spacing between characters can be specified. Sequences can be prepared and high quality output produced on a plotter in a short period of time, making the program advantageous to use over typing, computer printing, or preparation by a graphics department.  相似文献
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The problem of determining the sequence of a biopolymer from its fragments is stated in mathematical terms. Using concrete properties of a free monoid, certain general classes of biopolymers are shown to be insolvable from fragment data produced by complete digestion where enzymes specific for any possible combination of chemical bonds are employed. The authors are grateful to Dr. Malcolm W. Oliphant, Department of Mathematics, Georgetown University, for his comments and suggestions.  相似文献
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