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A H Neilson A S Allard C Lindgren M Remberger 《Applied and environmental microbiology》1987,53(10):2511-2519
Metabolically stable consortia of anaerobic bacteria obtained by enrichment of sediment samples with 3,4,5-trimethoxybenzoate (TMBA), 3,4,5-trihydroxybenzoate (gallate [GA]), or 5-chlorovanillin (CV) were used to study the anaerobic transformation of a series of chloroveratroles, chloroguaiacols, and chlorocatechols used as cosubstrates. Experiments were carried out with growing cultures, and the following pathways were demonstrated for metabolism of the growth substrates: (i) TMBA produced GA, which was further degraded without the formation of aromatic intermediates; (ii) GA formed pyrogallol, which was stable to further transformation; and (iii) CV was degraded by a series of steps involving de-O-methylation, oxidation of the aldehyde group, and decarboxylation to 3-chlorocatechol before ring cleavage. Mono-de-O-methylation of the cosubstrates occurred rapidly in the order 4,5,6-trichloroguaiacol greater than 3,4,5-trichloroguaiacol approximately 3,4,5-trichloroveratrole approximately tetrachloroveratrole greater than tetrachloroguaiacol and was concomitant with degradation of the growth substrates. For the polymethoxy compounds--chloroveratroles, 1,2,3-trichloro-4,5,6-trimethoxybenzene, and 4,5,6-trichlorosyringol--de-O-methylation took place sequentially. The resulting chlorocatechols were stable to further transformation until the cultures had exhausted the growth substrates; selective dechlorination then occurred with the formation of 3,5-dichlorocatechol from 3,4,5-trichlorocatechol and of 3,4,6-trichlorocatechol from tetrachlorocatechol. 2,4,5-, 2,4,6-, and 3,4,5-trichoroanisole and 2,3,4,5-tetrachloroanisole were de-O-methylated, but the resulting chlorophenols were resistant to dechlorination. These results extend those of a previous study with spiked sediment samples and their endogenous microflora and illustrate some of the transformations of chloroguaiacols and chlorocatechols which may be expected to occur in anaerobic sediments. 相似文献
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This study was designed to investigate spatial and temporal variation in Gelidium canariensis populations at two shores in
northern Gran Canaria during two years. Spatial scales ranged from some hundred meters (distance between shores), 10 to 30
m (distance between plots) to less than 3 m (distance between quadrats). Gelidium individuals were defined as distinct Gelidium
clumps. The results show a significant difference in size of clumps between shores, but not on the smaller spatial scales.
No significant temporal variation was found. There was no significant temporal or spatial variation in standing crop or density
(counts made in quadrats where Gelidium was present, rather than counts for the total shore). Sporophytic and gametophytic
clumps were also distinguished by identifying reproductive structures in the field. The total proportion of sporophytes was
larger than the proportion of gametophytes, but at a smaller scale there could be a shift in dominance. The survival rate
of clumps was similar between shores with a mean survival rate of 85%, but there was a significant difference in recruitment
between shores. The results indicate a stable population structure.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Receiver operating characteristic (ROC) curves are used to describe the performance of diagnostic procedures. This paper proposes a simple method for the statistical comparison of two ROC curves derived from the same set of patients and the same set of healthy subjects. Generalization to studies involving more than two screening factors is straightforward. This method does not require the calculation of variances of the areas or difference of areas under the curves. 相似文献
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