全文获取类型
收费全文 | 2445篇 |
免费 | 207篇 |
国内免费 | 4篇 |
出版年
2022年 | 9篇 |
2021年 | 42篇 |
2020年 | 18篇 |
2019年 | 19篇 |
2018年 | 25篇 |
2017年 | 20篇 |
2016年 | 56篇 |
2015年 | 104篇 |
2014年 | 122篇 |
2013年 | 119篇 |
2012年 | 181篇 |
2011年 | 183篇 |
2010年 | 106篇 |
2009年 | 88篇 |
2008年 | 122篇 |
2007年 | 106篇 |
2006年 | 118篇 |
2005年 | 97篇 |
2004年 | 115篇 |
2003年 | 101篇 |
2002年 | 87篇 |
2001年 | 78篇 |
2000年 | 83篇 |
1999年 | 59篇 |
1998年 | 28篇 |
1997年 | 24篇 |
1996年 | 21篇 |
1995年 | 13篇 |
1994年 | 23篇 |
1993年 | 8篇 |
1992年 | 31篇 |
1991年 | 44篇 |
1990年 | 22篇 |
1989年 | 36篇 |
1988年 | 27篇 |
1987年 | 47篇 |
1986年 | 27篇 |
1985年 | 36篇 |
1984年 | 30篇 |
1983年 | 19篇 |
1982年 | 16篇 |
1981年 | 12篇 |
1980年 | 7篇 |
1979年 | 22篇 |
1978年 | 15篇 |
1977年 | 17篇 |
1976年 | 8篇 |
1975年 | 12篇 |
1974年 | 15篇 |
1973年 | 9篇 |
排序方式: 共有2656条查询结果,搜索用时 31 毫秒
1.
2.
K. Y. E. Leung M. van Stralen M. G. Danilouchkine G. van Burken M. L. Geleijnse J. H. C. Reiber N. de Jong A. F. W. van der Steen J. G. Bosch 《Netherlands heart journal》2011,19(6):307-310
Real-time three-dimensional (3D) ultrasound imaging has been proposed as an alternative for two-dimensional stress echocardiography for assessing myocardial dysfunction and underlying coronary artery disease. Analysis of 3D stress echocardiography is no simple task and requires considerable expertise. In this paper, we propose methods for automated analysis, which may provide a more objective and accurate diagnosis. Expert knowledge is incorporated via statistical modelling of patient data. Methods for identifying anatomical views, detecting endocardial borders, and classification of wall motion are described and shown to provide favourable results. We also present software developed especially for analysis of 3D stress echocardiography in clinical practice. Interobserver agreement in wall motion scoring is better using the dedicated software (96%) than commercially available software not dedicated for this purpose (79%). The developed tools may provide useful quantitative and objective parameters to assist the clinical expert in the diagnosis of left ventricular function. 相似文献
3.
It is hypothesized that since protein α-amylase inhibitor (α-AI) and stimulator might be present together in red kidney bean
(Phaseolus vulgaris L.) seeds, their in vitro interactions might influence their detection and quantification. Assay of α-AI using extracts from the embryonic axes revealed
an unexpected finding in that the extracts stimulated rather than inhibited α-amylase activity. The cotyledon extracts exhibited
inhibitory or enhancement effect on α-amylase activity depending on whether prior to the α-amylase assay they had been boiled
for 10 min or not. Phytohemagglutinin (PHA-L in particular) is implicated in the present study as a stimulator of α-amylase
activity co-extracted with α-AI from red kidney bean cotyledons. The importance of these findings is discussed in relation
to the possible widespread occurrence of protein α-amylase stimulator in seeds and other plant parts. 相似文献
4.
Amy Chapman Gonzalo Gonzales Wayne R. Burrowes Paul Assanah Bernadette Iannone Michael K. Leung George B. Stefano 《Cellular and molecular neurobiology》1984,4(2):143-155
In Mytilus and Leucophaea the high-affinity binding site density is significantly lower in old animals than in young animals, whereas the low-affinity site density remains unchanged. In Mytilus the estimated met-enkephalin and met-enkephalin-Arg6-Phe7 levels are significantly higher in old than in young animals. In Leucophaea only the met-enkephalin level can be determined, and it is also higher in old animals. The decrease in the high-affinity binding site density and the corresponding increase in endogenous enkephalin levels suggest the existence of an opioid compensatory mechanism associated with the aging process. In Mytilus there is a demonstrated decrease with age in intraganglionic dopamine levels in response to applied opiates. In addition, the inhibition of dopamine-stimulated adenylate cyclase activity by opiates also decreases in older animals. In Leucophaea the sex difference in opioid binding densities diminishes with age. 相似文献
5.
Four myeloid cell lines (M1, WEHI-3B D+, FDC-P1, and 32D) were screened for the presence of J11d antigen. One of these cell lines, the myeloid leukemia M1, was found to express a high level of J11d antigen on the cell surface. Recombinant mouse leukemic inhibitory factor (rm-LIF), recombinant human LIF (rh-LIF), and steroids (hydrocortisone and dexamethasone) could induce M1 cells to undergo monocytic differentiation. The level of J11d antigen was greatly reduced after treatment of the cells with LIF or steroids. Western blotting revealed that the apparent molecular weight of the J11d antigen on M1 cells was 45-48 kDa. Furthermore, the level of J11d mRNA was also reduced during LIF-induced differentiation of M1 cells. 相似文献
6.
Q Yuan W M Jiang D Hollander E Leung J D Watson G W Krissansen 《Biochemical and biophysical research communications》1991,176(3):1443-1449
A cDNA clone encoding the N-terminal sequence of the murine integrin beta 7 subunit, a novel member of the leukocyte cell adhesion molecule subset (Leu-CAM), has been isolated. An N-terminal region of 13 contiguous amino acids deduced from the cDNA shows complete identity with the N-terminus of the 120 kDa subunit of the M290 antigen, a surface molecule found highly expressed on mouse intestinal intraepithelial lymphocytes (IEL). This unexpected result focuses two previously unconnected areas of research and suggests that integrins may have a special role to play in the defence of the gut mucosa. 相似文献
7.
A simple and rapid method for preparing [32P]adenosine 3'5'-cyclic monophosphate (cAMP) is described. A culture of an Escherichia coli mutant which excretes cAMP about 150 times faster than does a wild-type strain was incubated overnight with [32P]orthophosphate of high specific activity (e.g., 4000 Ci/mol (1 Ci = 37 GBq). The [32P]cAMP which accumulated extracellularly was then purified to 99.9% radiochemical purity in less than 4 h by adsorption to charcoal and alumina column chromatography. A two-dimensional chromatography system using a PEI-cellulose plate is also described which should prove useful for studying cAMP metabolism with 32P- or 3H-labeled cAMP or ATP. 相似文献
8.
In vivo-induced suppression of T cell proliferation: the relationship between the specificity of induction and control 总被引:1,自引:0,他引:1
We have previously shown that sc immunization of C57BL/10 (H-2b) mice with the tobacco mosaic virus protein (TMVP) or with its tryptic peptide number 8, representing residues 93-112 of TMVP, induces T cells which proliferate in vitro in response to TMVP and to peptide 8. In contrast, immunization of B10.BR (H-2k) mice either with TMVP or with peptide 8 induces T cells which respond in vitro to the homologous but not the heterologous Ag. In the present article , we report that in the B10.BR (H-2k) strain, ip prepriming with (TMVP) 7 days prior to sc immunization with peptide 8 causes a drastic reduction in the in vitro proliferative response of peptide 8-specific T cells while no such effect is seen in the congenic C57BL/10 (H-2b) strain. This suppression of T cell responsiveness can be transferred with TMVP-primed spleen cells. Moreover, deleting T cells from the transferred spleen cells abrogates the suppressive effect. In both H-2 haplotypes, ip prepriming with peptide 8 causes suppression of the proliferative T cell response induced by subsequent immunization with peptide 8. This prepriming has no effect on the response to TMVP immunization of B10.BR mice but does effect the response of C57BL/10 mice. Using various synthetic peptides to analyze the specificity of the suppression, we have determined that (1) T cells involved in the suppression of the proliferative T cell response to a single peptide determinant do not suppress the proliferative T cell response to other determinants on the protein antigen and (2) these T cells with suppressor function, and proliferating T cells which are ultimately regulated, can exhibit specificity for the same epitope. These studies suggest that there may exist fundamental differences as to how T cells which participate in suppression an proliferating T cells (which include mainly T helper cells) recognize protein antigens. 相似文献
9.
G A Dunaway G L Leung J R Thrasher M D Cooper 《The Journal of biological chemistry》1978,253(20):7460-7463
Earlier work demonstrated that the activity of liver phosphofructokinase (PFK-L2) and immunoreactive PFK-L2 were decreased in diabetic rats and increased to normal or super-normal amounts following insulin treatment (Dunaway, G.A., and Weber, G., (1974) Arch. Biochem. Biophys. 162, 629-637). This report indicates that the decrease in levels of PFK-L2 in diabetic rats is a result of an accelerated degradation rate while the synthetic rate remains nearly normal. Following insulin treatment, the rate of PFK-L2 synthesis is enhanced 2-fold, whereas the rate of degradation appears to be greatly diminished. An inverse relationship is shown to exist between the PFK-L2 levels and the rates of PFK-L2 degradation, suggesting that the levels of PFK-L2 are primarily regulated by degradation rate. In addition, the levels of the PFK-L2 peptide stabilizing factor are inversely proportional to rates of PFK-L2 degradation. These results indicate that insulin mediates the rate of degradation of PFK-L2 by controlling the level of the peptide stabilizing factor. 相似文献
10.