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排序方式: 共有5146条查询结果,搜索用时 15 毫秒
1.
Joyce S. F. D. de Almeida Teobaldo R. Cuya Guizado Ana P. Guimarães Teodorico C. Ramalho Arlan S. Gonçalves Martijn C. de Koning 《Journal of biomolecular structure & dynamics》2016,34(12):2632-2642
In the present work, we performed docking and molecular dynamics simulations studies on two groups of long-tailored oximes designed as peripheral site binders of acetylcholinesterase (AChE) and potential penetrators on the blood brain barrier. Our studies permitted to determine how the tails anchor in the peripheral site of sarin-inhibited human AChE, and which aminoacids are important to their stabilization. Also the energy values obtained in the docking studies corroborated quite well with the experimental results obtained before for these oximes. 相似文献
2.
Surface Plasmon Resonance Imaging-Based Protein Array Chip System for Monitoring a Hexahistidine-Tagged Protein during Expression and Purification 下载免费PDF全文
Hyeon-Su Ro Sun Ok Jung Byung Hoon Kho Hyung Pyo Hong Jae Sung Lee Yong-Beom Shin Min Gon Kim Bong Hyun Chung 《Applied microbiology》2005,71(2):1089-1092
A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins. 相似文献
3.
Natalia Cadaxo Rochael Luize Gon?alves Lima Sandra Maria Pereira de Oliveira Marcello André Barcinski Elvira Maria Saraiva Robson Queiroz Monteiro Lucia Helena Pinto-da-Silva 《Memórias do Instituto Oswaldo Cruz》2013,108(6):679-685
Leishmania parasites expose phosphatidylserine (PS) on their
surface, a process that has been associated with regulation of host''s immune
responses. In this study we demonstrate that PS exposure by metacyclic
promastigotes of Leishmania amazonensis favours blood
coagulation. L. amazonensis accelerates in vitro coagulation of
human plasma. In addition, L. amazonensis supports the assembly
of the prothrombinase complex, thus promoting thrombin formation. This process
was reversed by annexin V which blocks PS binding sites. During blood meal,
Lutzomyia longipalpis sandfly inject saliva in the bite
site, which has a series of pharmacologically active compounds that inhibit
blood coagulation. Since saliva and parasites are co-injected in the host during
natural transmission, we evaluated the anticoagulant properties of sandfly
saliva in counteracting the procoagulant activity of L.
amazonensis . Lu. longipalpis saliva reverses
plasma clotting promoted by promastigotes. It also inhibits thrombin formation
by the prothrombinase complex assembled either in phosphatidylcholine (PC)/PS
vesicles or in L. amazonensis . Sandfly saliva inhibits factor
X activation by the intrinsic tenase complex assembled on PC/PS vesicles and
blocks factor Xa catalytic activity. Altogether our results show that metacyclic
promastigotes of L. amazonensis are procoagulant due to PS
exposure. Notably, this effect is efficiently counteracted by sandfly
saliva. 相似文献
4.
5.
Carlo L. Seifert Martin Volf Leonardo R. Jorge Tomokazu Abe Grace Carscallen Pavel Drozd Rajesh Kumar Greg P.A. Lamarre Martin Libra Maria E. Losada Scott E. Miller Masashi Murakami Geoffrey Nichols Petr Pyszko Martin igut David L. Wagner Vojtch Novotný 《Ecology and evolution》2020,10(24):14137
- Assemblages of insect herbivores are structured by plant traits such as nutrient content, secondary metabolites, physical traits, and phenology. Many of these traits are phylogenetically conserved, implying a decrease in trait similarity with increasing phylogenetic distance of the host plant taxa. Thus, a metric of phylogenetic distances and relationships can be considered a proxy for phylogenetically conserved plant traits and used to predict variation in herbivorous insect assemblages among co‐occurring plant species.
- Using a Holarctic dataset of exposed‐feeding and shelter‐building caterpillars, we aimed at showing how phylogenetic relationships among host plants explain compositional changes and characteristics of herbivore assemblages.
- Our plant–caterpillar network data derived from plot‐based samplings at three different continents included >28,000 individual caterpillar–plant interactions. We tested whether increasing phylogenetic distance of the host plants leads to a decrease in caterpillar assemblage overlap. We further investigated to what degree phylogenetic isolation of a host tree species within the local community explains abundance, density, richness, and mean specialization of its associated caterpillar assemblage.
- The overlap of caterpillar assemblages decreased with increasing phylogenetic distance among the host tree species. Phylogenetic isolation of a host plant within the local plant community was correlated with lower richness and mean specialization of the associated caterpillar assemblages. Phylogenetic isolation had no effect on caterpillar abundance or density. The effects of plant phylogeny were consistent across exposed‐feeding and shelter‐building caterpillars.
- Our study reveals that distance metrics obtained from host plant phylogeny are useful predictors to explain compositional turnover among hosts and host‐specific variations in richness and mean specialization of associated insect herbivore assemblages in temperate broadleaf forests. As phylogenetic information of plant communities is becoming increasingly available, further large‐scale studies are needed to investigate to what degree plant phylogeny structures herbivore assemblages in other biomes and ecosystems.
6.
Biogeographical analyses are applied to skipper (Hesperioidea) presence/absence data from the Western Mediterranean mainland
and the three largest islands (Sardinia, Corsica and Sicily) in order to identify potential conservation issues. The analyses
performed on species, both collectively and individually, indicate that regional species richness and occurrence in the Mediterranean
zone are largely predicted by latitude and area but that islands have impoverished faunas. Several species, predicted to be
present on these islands from logistic regression of their continental distributions, are actually absent. The number of species
predicted to be present from logistic regression analyses for each island, closely matched the number of species predicted
to occur in regional-focused multiple regression analysis. This suggests that missing species have been identified. When compared
with species that occur in Sicily and Corsica, the missing species are shown to differ for ecological traits, mainly those
linked to altitudinal tolerance. No ecological distinctions were disclosed for Sardinian skippers suggesting a mainly stochastic
colonisation. These results, and those from an analogous study carried out on Papilionoidea, point to Hesperioidea having
(i) overall more impoverished faunas on islands and (ii) being subject to stochastic or historical colonisation events more
than Papilionoidea. Species not predicted to occur on islands based on their mainland distributions and ecological traits,
are foci for conservation attention. However, as many species becoming extinct on the islands may be irreplaceable, all species,
in particular the Sardinian ones, deserve to be conserved. 相似文献
7.
Sacramento Doralice Rodrigues Coelho Rosalie Reed Rodrigues Wigg Márcia Dutra Toledo Luna Linhares Luiz Fernando de Matos dos Santos Marta Gonçalves Azevedo Soares Semêdo Luzia Teixeira de Ribeiro da Silva Antonio Jorge 《World journal of microbiology & biotechnology》2004,20(3):225-229
A promising producer of bioactive compounds isolated from a Brazilian tropical soil was tested for its range of antimicrobial
activities. Strain 606, classified as Streptomyces sp., could not be identified up to species level, suggesting a possible new taxon. The supernatant and 10 extracts and fractions,
obtained by extraction and chromatographic techniques, presented antimicrobial activity using antibiograms. The methanolic
fraction was highly active against pathogenic bacteria, phytopathogenic fungi and the human pathogenic yeast Candida albicans. It also possessed high antiviral activity inhibiting the propagation of an acyclovir-resistant herpes simplex virus type
1 strain on HEp-2 cells at non-cytotoxic concentration. The strong cytotoxic effect suggests an antitumour action.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
8.
Claudia N. Gonzalez-Brambila Leonardo Reyes-Gonzalez Francisco Veloso Miguel Angel Perez-Angón 《PloS one》2016,11(3)
This paper analyzes science productivity for nine developing countries. Results show that these nations are reducing their science gap, with R&D investments and scientific impact growing at more than double the rate of the developed world. But this “catching up” hides a very uneven picture among these nations, especially on what they are able to generate in terms of impact and output relative to their levels of investment and available resources. Moreover, unlike what one might expect, it is clear that the size of the nations and the relative scale of their R&D investments are not the key drivers of efficiency. 相似文献
9.
10.
The occurrence of a pre-imaginal caste determination represents a sort of “point of no return” to eusociality. In some social
insect taxa, including Polistes species, the occurrence of distinct queen and worker castes is still debated. Before this report, no clear morphological
differences between reproductive and non-reproductive individuals were known. Here we show that several pre-imaginal morphological
differences of the Van der Vecht organ occur between foundresses (queens) and workers. Geometric morphometrics revealed that
queens are characterized by shape deformations of this organ, which is responsible for a typical allometric growth of the
secretory area. This organ is predicted to be larger in foundresses compared to workers because its secretion is involved
in defense against ants, in nestmate recognition and in preventing workers from challenging for direct reproduction. The results
presented here indicate the existence of an incipient morphological caste determination Polistes gallicus and suggest that this species may have passed the “point of no return” for eusociality. 相似文献