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1.
Isolation and characterization of maize genes coding for zein proteins of the 21000 dalton size class 总被引:4,自引:2,他引:2 下载免费PDF全文
Cloning in lambda gt WES phage of EcoRI fragments from maize seedlings DNA led to the isolation of four fragments containing genes coding for 21000 dalton zein proteins. The zein genes, identified by electron microscopic analysis, do not contain intervening sequences detectable by this method. The flanking sequences were analyzed by restriction sites mapping and hybridization and showed areas of homology between each other and with sequences surrounding a previously isolated gene of the 19000 dalton zein class. 相似文献
2.
James I. Langridge A. Gareth Brenton Terry J. Walton Frank M. Harris Russell P. Newton 《Nucleosides, nucleotides & nucleic acids》2013,32(3-5):739-742
Abstract Fast atom bombardment mass spectrometry in concert with collisionally-induced dissociation and mass-analysed kinetic energy spectrum scanning has enabled unequivocal identification of putative cyclic nucleotides in tissue extracts; quantitative analyses have enabled kinetic studies of cyclic nucleotide-related enzymes with multi-component monitoring, and dynamic spectrometric systems provide a potential means of continuous assay for these enzymes. 相似文献
3.
Irwin D. Kuntz Jeffrey M. Blaney Stuart J. Oatley Robert Langridge Thomas E. Ferrin 《Journal of molecular biology》1982,161(2):269-288
We describe a method to explore geometrically feasible alignments of ligands and receptors of known structure. Algorithms are presented that examine many binding geometries and evaluate them in terms of steric overlap. The procedure uses specific molecular conformations. A method is included for finding putative binding sites on a macromolecular surface.Results are reported for two systems: the heme-myoglobin interaction and the binding of thyroid hormone analogs to prealbumin. In each case the program finds structures within 1 Å of the X-ray results and also finds distinctly different geometries that provide good steric fits. The approach seems well-suited for generating starting conformations for energy refinement programs and interactive computer graphics routines. 相似文献
4.
Multi-environment analysis and improved mapping of a yield-related QTL on chromosome 3B of wheat 总被引:1,自引:0,他引:1
Julien Bonneau Julian Taylor Boris Parent Dion Bennett Matthew Reynolds Catherine Feuillet Peter Langridge Diane Mather 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(3):747-761
Improved mapping, multi-environment quantitative trait loci (QTL) analysis and dissection of allelic effects were used to define a QTL associated with grain yield, thousand grain weight and early vigour on chromosome 3BL of bread wheat (Triticum aestivum L.) under abiotic stresses. The QTL had pleiotropic effects and showed QTL x environment interactions across 21 diverse environments in Australia and Mexico. The occurrence and the severity of water deficit combined with high temperatures during the growing season affected the responsiveness of this QTL, resulting in a reversal in the direction of allelic effects. The influence of this QTL can be substantial, with the allele from one parent (RAC875) increasing grain yield by up to 12.5 % (particularly in environments where both heat and drought stress occurred) and the allele from the other parent (Kukri) increasing grain yield by up to 9 % in favourable environments. With the application of additional markers and the genotyping of additional recombinant inbred lines, the genetic map in the QTL region was refined to provide a basis for future positional cloning. 相似文献
5.
6.
Amanda T. Langridge Emma J. Glasson Natasha Nassar Peter Jacoby Craig Pennell Ronald Hagan Jenny Bourke Helen Leonard Fiona J. Stanley 《PloS one》2013,8(1)
Background
As well as being highly comorbid conditions, autism spectrum disorders (ASD) and intellectual disability (ID) share a number of clinically-relevant phenomena. This raises questions about similarities and overlap in diagnosis and aetiological pathways that may exist for both conditions.Aims
To examine maternal conditions and perinatal factors for children diagnosed with an ASD, with or without ID, and children with ID of unknown cause, compared with unaffected children.Methods
The study population comprised all live singleton births in Western Australia (WA) between January 1984 and December 1999 (N = 383,153). Univariate and multivariate multinomial logistic regression models were applied using a blocked modelling approach to assess the effect of maternal conditions, sociodemographic factors, labour and delivery characteristics and neonatal outcomes.Results
In univariate analyses mild-moderate ID was associated with pregnancy hypertension, asthma, urinary tract infection, some types of ante-partum haemorrhage, any type of preterm birth, elective C-sections, breech presentation, poor fetal growth and need for resuscitation at birth, with all factors showing an increased risk. Severe ID was positively associated with poor fetal growth and need for resuscitation, as well as any labour or delivery complication. In the multivariate analysis no maternal conditions or perinatal factors were associated with an increased risk of ASD without ID. However, pregnancy hypertension and small head circumference were associated with a reduced risk (OR = 0.64, 95% CI: 0.43, 0.94; OR = 0.58, 95% CI: 0.34, 0.96, respectively). For ASD with ID, threatened abortion before 20 weeks gestation and poor fetal growth were associated with an increased risk.Conclusion
Findings show that indicators of a poor intrauterine environment are associated with an elevated risk of ID, while for ASD, and particularly ASD without ID, the associations are much weaker. As such, these findings highlight the importance of accounting for the absence or presence of ID when examining ASD, if we are to improve our understanding of the causal pathways associated with these conditions. 相似文献7.
8.
Mutation spectra and the neutrality of mutations 总被引:3,自引:0,他引:3
J Langridge 《Australian journal of biological sciences》1974,27(3):309-319
9.
Entomopoxvirus (EPV) occlusion bodies were isolated from virus infected nymphs of the grasshoppers Melanoplus sanguinipes, Arphia conspirsa, and Phoetaliotes nebrascensis. Separation of the viral structural proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis gave unique protein patterns for each of the three viruses. An occlusion body protein of approximately 100,000 MW was isolated from each virus. Cleavage of viral DNA with HinddIII and BamHI restriction endonucleases and separation of the fragments by agarose gel electrophoresis gave different DNA fragment patterns for each of the three entomopoxviruses. Molecular weight estimates of 120 × 106 for M. sanguinipes EPV DNA, 129 × 106 for A. conspirsa EPV DNA, and 125 × 106 for P. nebrascensis EPV DNA were calculated from the sizes of the viral DNA fragments. Approximately 55% base sequence homology was detected by Southern hybridization of α-32P-labeledM. sanguinipes EPV DNA with P. nebrascensis DNA. No base sequence homology was detected by Southern hybridization of labeled M. sanguinipes EPV DNA to Othnonius batesi EPV DNA (Coleoptera), Amsacta moorei EPV DNA (Lepidoptera), Euxoa auxiliaris EPV DNA (Lepidoptera), and vaccinia virus DNA fragments. 相似文献
10.
Eun-Ah Shin Yong Keun Park Kang Oh Lee William H. R. Langridge Jin-Yong Lee 《Molecular biotechnology》2009,43(2):138-147
Periodontal disease caused by the gram-negative oral anaerobic bacterium Porphyromonas gingivalis is thought to be initiated by the binding of P. gingivalis fimbrial protein to saliva-coated oral surfaces. To assess whether biologically active fimbrial antigen can be synthesized
in edible plants, a cDNA fragment encoding the C-terminal binding portion of P. gingivalis fimbrial protein, fimA (amino acids 266–337), was cloned behind the mannopine synthase promoter in plant expression vector pPCV701. The plasmid
was transferred into potato (Solanum tuberosum) leaf cells by Agrobacterium tumefaciens in vivo transformation methods. The fimA cDNA fragment was detected in transformed potato leaf genomic DNA by PCR amplification methods. Further, a novel immunoreactive
protein band of ~6.5 kDa was detected in boiled transformed potato tuber extracts by acrylamide gel electrophoresis and immunoblot
analysis methods using primary antibodies to fimbrillin, a monomeric P. gingivalis fimbrial subunit. Antibodies generated against native P. gingivalis fimbriae detected a dimeric form of bacterial-synthesized recombinant FimA(266–337) protein. Further, a protein band of ~160 kDa
was recognized by anti-FimA antibodies in undenatured transformed tuber extracts, suggesting that oligomeric assembly of plant-synthesized
FimA may occur in transformed plant cells. Based on immunoblot analysis, the maximum amount of FimA protein synthesized in
transformed potato tuber tissues was approximately 0.03% of total soluble tuber protein. Biosynthesis of immunologically detectable
FimA protein and assembly of fimbrial antigen subunits into oligomers in transformed potato tuber tissues demonstrate the
feasibility of producing native FimA protein in edible plant cells for construction of plant-based oral subunit vaccines against
periodontal disease caused by P. gingivalis. 相似文献