全文获取类型
收费全文 | 436篇 |
免费 | 36篇 |
国内免费 | 1篇 |
出版年
2022年 | 4篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2016年 | 12篇 |
2015年 | 17篇 |
2014年 | 13篇 |
2013年 | 19篇 |
2012年 | 18篇 |
2011年 | 22篇 |
2010年 | 18篇 |
2009年 | 14篇 |
2008年 | 19篇 |
2007年 | 10篇 |
2006年 | 17篇 |
2005年 | 13篇 |
2004年 | 19篇 |
2003年 | 16篇 |
2002年 | 15篇 |
2001年 | 22篇 |
2000年 | 17篇 |
1999年 | 13篇 |
1998年 | 4篇 |
1997年 | 9篇 |
1996年 | 14篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1993年 | 3篇 |
1992年 | 9篇 |
1991年 | 6篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1981年 | 6篇 |
1980年 | 3篇 |
1979年 | 6篇 |
1978年 | 3篇 |
1977年 | 5篇 |
1973年 | 6篇 |
1972年 | 4篇 |
1971年 | 3篇 |
1970年 | 4篇 |
1967年 | 3篇 |
1918年 | 2篇 |
排序方式: 共有473条查询结果,搜索用时 41 毫秒
1.
2.
3.
4.
RNA and protein synthesis by sterile rat-liver mitochondria 总被引:2,自引:0,他引:2
5.
Protease accessibility experiments were employed to localize a PhoE-LacZ hybrid protein, encompassing a large N-terminal fragment of the outer membrane PhoE protein of E. coli, fused to beta-galactosidase, at the subcellular level. In previous studies, this protein was shown to co-fractionate with the outer membrane, whereas immunocytochemical methods suggested a cytoplasmic location. The present results confirm the latter localization. Moreover, it appears that a minor amount of hybrid protein spans the inner membrane, with the PhoE moiety in the periplasm and the beta-galactosidase moiety in the cytoplasm. These membrane-spanning proteins might be responsible for the lethal jamming of the export machinery, observed upon induction of synthesis of the protein. 相似文献
6.
NMR studies of the low-density lipoprotein receptor-binding peptide of apolipoprotein E bound to dodecylphosphocholine micelles. 下载免费PDF全文
D. Clayton I. M. Brereton P. A. Kroon R. Smith 《Protein science : a publication of the Protein Society》1999,8(9):1797-1805
Circular dichroism and NMR spectroscopy have been used to determine the structure of the low-density lipoprotein (LDL) receptor-binding peptide, comprising residues 130-152, of the human apolipoprotein E. This peptide has little persistent three-dimensional structure in solution, but when bound to micelles of dodecylphosphocholine (DPC) it adopts a predominantly alpha-helical structure. The three-dimensional structure of the DPC-bound peptide has been determined by using 1H-NMR spectroscopy: the structure derived from NOE-based distance constraints and restrained molecular dynamics is largely helical. The derived phi and psi angle order parameters show that the helical structure is well defined but with some flexibility that causes the structures not to be superimposable over the full peptide length. Deuterium exchange experiments suggest that many peptide amide groups are readily accessible to the solvent, but those associated with hydrophobic residues exchange more slowly, and this helix is thus likely to be positioned on the surface of the DPC micelles. In this conformation the peptide has one hydrophobic face and two that are rich in basic amino acid side chains. The solvent-exposed face of the peptide contains residues previously shown to be involved in binding to the LDL receptor. 相似文献
7.
8.
Kristina Wallenius Tobias Kroon Therese Hagstedt Lars Löfgren Maria Sörhede-Winzell Jeremie Boucher Daniel Lindén Nicholas D. Oakes 《Journal of lipid research》2022,63(3):100176
Sodium-glucose cotransporter 2 (SGLT2) inhibitors have been shown to increase ketone bodies in patients with type 2 diabetes; however, the underlying mechanisms have not been fully elucidated. Here we examined the effect of the SGLT2 inhibitor dapagliflozin (1 mg/kg/day, formulated in a water, PEG400, ethanol, propylene glycol solution, 4 weeks) on lipid metabolism in obese Zucker rats. Fasting FFA metabolism was assessed in the anesthetized state using a [9,10-3H(N)]-palmitic acid tracer by estimating rates of plasma FFA appearance (Ra), whole-body FFA oxidation (Rox), and nonoxidative disposal (Rst). In the liver, clearance (Kβ-ox) and flux (Rβ-ox) of FFA into β-oxidation were estimated using [9,10-3H]-(R)-bromopalmitate/[U-14C]palmitate tracers. As expected, dapagliflozin induced glycosuria and a robust antidiabetic effect; treatment reduced fasting plasma glucose and insulin, lowered glycated hemoglobin, and increased pancreatic insulin content compared with vehicle controls. Dapagliflozin also increased plasma FFA, Ra, Rox, and Rst with enhanced channeling toward oxidation versus storage. In the liver, there was also enhanced channeling of FFA to β-oxidation, with increased Kβ-ox, Rβ-ox and tissue acetyl-CoA, compared with controls. Finally, dapagliflozin increased hepatic HMG-CoA and plasma β-hydroxybutyrate, consistent with a specific enhancement of ketogenesis. Since ketogenesis has not been directly measured, we cannot exclude an additional contribution of impaired ketone body clearance to the ketosis. In conclusion, this study provides evidence that the dapagliflozin-induced increase in plasma ketone bodies is driven by the combined action of FFA mobilization from adipose tissue and diversion of hepatic FFA toward β-oxidation. 相似文献
9.
Ohne Zusammenfassungmit einem Stammbaum 相似文献
10.