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1.
New methods of analysing genetic data provide powerful tools for quantifying dispersal patterns and reconstructing population
histories. Here we examine the population structure of the bumblebee Bombus hortorum in a model island system, the Western Isles of Scotland, using microsatellite markers. Following declines in other species,
B. hortorum is the only remaining long-tongued bumblebee species found in much of Europe, and thus it is of particular ecological importance.
Our data suggest that populations of B. hortorum in western Scotland exist as distinct genetic clusters occupying groups of nearby islands. Population structuring was higher
than for other bumblebee species which have previously been studied in this same island group (Fst = 0.16). Populations showed significant isolation by distance. This relationship was greatly improved by using circuit theory
to allow dispersal rates to differ over different landscape features; as we would predict, sea appears to provide far higher
resistance to dispersal than land. Incorporating bathymetry data improved the fit of the model further; populations separated
by shallow seas are more genetically similar than those separated by deeper seas. We argue that this probably reflects events
following the last ice age when the islands were first colonized by this bee species (8,500–5,000 ybp), when the sea levels
were lower and islands separated by shallow channels would have been joined. In the absence of significant gene flow these
genetic clusters appear to have since diverged over the following 5,000 years and arguably may now represent locally adapted
races, some occurring on single islands. 相似文献
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N. J. Silk S. Denby G. Lewis M. Kuiper D. Hatton R. Field F. Baganz Gary J. Lye 《Biotechnology letters》2010,32(1):73-78
Recently we have demonstrated batch suspension culture of mammalian cells in microwell plates. Here we describe a method for
fed-batch culture of an industrially relevant GS-CHO (Glutamine Synthetase-Chinese Hamster Ovary) cell line in shaken 24-standard
round well (24-SRW) plates. Use of a commercially available ‘sandwich lid’ and appropriate dilution of the bolus feeds counteracted
liquid evaporation from the wells resulting in similar cell growth and antibody formation kinetics in both 24-SRW plates (800 μl)
and shaken flasks (50 ml). Peak viable cell densities obtained were 8 ± 0.5 × 106 and 9 ± 1.3 × 106 ml−1, respectively, while comparable final titres of a whole IgG of approximately 1.5 g l−1 were recorded. Use of microwells provides at least a 50-fold reduction in medium requirements compared to shake-flask and
other culture devices currently used in early stage cell culture process development. The ability to run multiple wells in
parallel and to automate culture operation also offers considerable enhancements in experimental throughput. 相似文献
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Guinever E. Imperio Mohsen Javam Phetcharawan Lye Andrea Constantinof Caroline E. Dunk Fernando M. Reis Stephen J. Lye William Gibb Stephen G. Matthews Tania Maria Ortiga‐Carvalho Enrrico Bloise 《Journal of cellular and molecular medicine》2019,23(1):610-618
The ATP‐binding cassette (ABC) transporters control placental transfer of several nutrients, steroids, immunological factors, chemicals, and drugs at the maternal‐fetal interface. We and others have demonstrated a gestational age‐dependent expression pattern of two ABC transporters, P‐glycoprotein and breast cancer resistance protein throughout pregnancy. However, no reports have comprehensively elucidated the expression pattern of all 50 ABC proteins, comparing first trimester and term human placentae. We hypothesized that placental ABC transporters are expressed in a gestational‐age dependent manner in normal human pregnancy. Using the TaqMan® Human ABC Transporter Array, we assessed the mRNA expression of all 50 ABC transporters in first (first trimester, n = 8) and third trimester (term, n = 12) human placentae and validated the resulting expression of selected ABC transporters using qPCR, Western blot and immunohistochemistry. A distinct gene expression profile of 30 ABC transporters was observed comparing first trimester vs. term placentae. Using individual qPCR in selected genes, we validated the increased expression of ABCA1 (P < 0.01), ABCA6 (P < 0.001), ABCA9 (P < 0.001) and ABCC3 (P < 0.001), as well as the decreased expression of ABCB11 (P < 0.001) and ABCG4 (P < 0.01) with advancing gestation. One important lipid transporter, ABCA6, was selected to correlate protein abundance and characterize tissue localization. ABCA6 exhibited increased protein expression towards term and was predominantly localized to syncytiotrophoblast cells. In conclusion, expression patterns of placental ABC transporters change as a function of gestational age. These changes are likely fundamental to a healthy pregnancy given the critical role that these transporters play in the regulation of steroidogenesis, immunological responses, and placental barrier function and integrity. 相似文献
6.
Maino James L. Schouten Rafael Lye Jessica C. Umina Paul A. Reynolds Olivia L. 《Biological invasions》2022,24(7):2155-2167
Biological Invasions - The Spotted Lantern Fly (SLF), Lycorma delicatula (Hemiptera: Fulgoridae), is a sap feeding pest native to southeast Asia that has become a global biosecurity threat... 相似文献
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Paula H Suss Luiz Guilherme A Capriglione Fabiane Barchiki Lye Miyague Danielle Jackowski Letícia Fracaro Andressa V Schittini Alexandra C Senegaglia Carmen LK Rebelatto Márcia Olandoski Alejandro Correa Paulo RS Brofman 《Experimental biology and medicine (Maywood, N.J.)》2015,240(7):969-978
The development of new therapeutic strategies is necessary to reduce the worldwide social and economic impact of cardiovascular disease, which produces high rates of morbidity and mortality. A therapeutic option that has emerged in the last decade is cell therapy. The aim of this study was to compare the effect of transplanting human umbilical cord-derived stromal cells (UCSCs), human umbilical cord blood-derived endothelial cells (UCBECs) or a combination of these two cell types for the treatment of ischemic cardiomyopathy (IC) in a Wistar rat model. IC was induced by left coronary artery ligation, and baseline echocardiography was performed seven days later. Animals with a left ventricular ejection fraction (LVEF) of ≤40% were selected for the study. On the ninth day after IC was induced, the animals were randomized into the following experimental groups: UCSCs, UCBECs, UCSCs plus UCBECs, or vehicle (control). Thirty days after treatment, an echocardiographic analysis was performed, followed by euthanasia. The animals in all of the cell therapy groups, regardless of the cell type transplanted, had less collagen deposition in their heart tissue and demonstrated a significant improvement in myocardial function after IC. Furthermore, there was a trend of increasing numbers of blood vessels in the infarcted area. The median value of LVEF increased by 7.19% to 11.77%, whereas the control group decreased by 0.24%. These results suggest that UCSCs and UCBECs are promising cells for cellular cardiomyoplasty and can be an effective therapy for improving cardiac function following IC. 相似文献
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The ability of taxa to cross/hybridize is useful information for plant systematists and breeders. Crossability reflects reproductive isolation and the biological species concept stresses the need for reproductive isolation between species to maintain morphological distincness. For plant breeders knowledge on crossing ability facilitate selection of taxa for character improvement breeding. In this study, the crossing relationships and chromosome numbers within and among Ugandan species of Solanum sect. Solanum is studied by making 800 crosses involving 246 combinations. Less than half of these combinations were successful, producing F1 offspring. All studied accessions are self‐compatible and most accessions crossed readily with accessions of their own species. Interspecific crossings failed either to yield seeds, yielded F1 seeds that did not germinate, or resulted in F1s that did not have stainable pollen – implying a crossing barrier; or stainable pollen, but with chromosome numbers that indicated reproduction by apomixis. The results support the taxonomic treatment of Solanum based on classical, numerical and partly molecular evidences. The material studied represents eight Ugandan taxa: S. americanum, a diploid (2n = 2x = 24); five tetraploids (2n = 4x = 48) S. florulentum, S. memphiticum, S. tarderemotum, S. villosum ssp. villosum and S. villosum ssp. miniatum; and two hexaploids (2n = 6x = 72) S. scabrum subsp. scabrum and S. scabrum subsp. laevis. In addition to confirming the ploidy levels of the Ugandan accessions, the ploidy levels of S. florulentum, S. memphiticum and S. tarderemotum are reported for the first time. Non‐Ugandan material of Solanum sarrachoides was found to be diploid. Knowledge of the crossing behaviour and ploidy levels in Solanum will facilitate breeding for character improvement in these important species that are used commonly as food and/or medicine in eastern Africa. 相似文献
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