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1.
Kazuya Shirato 《Microbiology and immunology》2019,63(10):407-412
Loop‐mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real‐time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA. Recently, real‐time monitoring of fluorescence signals using a quenching primer and probe has improved the reliability of amplification signals. Here, methods of detecting LAMP amplicons are reviewed. 相似文献
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Kazuya Hayashi Masaru Terada Koya Mogi 《Bioscience, biotechnology, and biochemistry》2013,77(4):627-637
Some enzymatic properties of purified alkaline proteinase from Aspergillus sojae were investigated. The optimum pH for casein digestion was 11.0. The enzyme activity was almost completely lost at 60°C within ten minutes. At low temperature, the enzyme was highly stable at the range of pH 4.5 to 10.0. At 50°C, the most stable pH was around 6.0. None of metallic ions tested promoted the activity, but Hg2+ showed a remarkable inhibition. The Hg2+-treatment seemed to cause a large unfolding of the enzyme molecule. The enzyme was inhibited by potato inhibitor and a number of animal sera. Metal chelating reagents and sulfhydryl reagents tested had no effect on the activity, but DFP caused a marked inhibition. The sensitivity to DFP of the enzyme was about 1/300 of that of α-chymotrypsin. The enzyme was inhibited neither by TPCK nor by TLCK. As the result it was assumed that the structure of the active site of the enzyme is fairly different from that of trypsin, or of chymotrypsin. 相似文献
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Shingo Marumo Kazuya Sasaki Saburo Suzuki 《Bioscience, biotechnology, and biochemistry》2013,77(12):1931-1935
The relative stereochemistry of cervicarcin, an antitumor antibiotic, was determined as shown in 1, which represents the absolute stereochemistry also. 相似文献
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