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排序方式: 共有5278条查询结果,搜索用时 156 毫秒
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Yoshinari Maeda Kiyoshi Yoshimura Hiroto Matsui Yoshitaro Shindo Takao Tamesa Yukio Tokumitsu Noriaki Hashimoto Yoshihiro Tokuhisa Kazuhiko Sakamoto Kouhei Sakai Yutaka Suehiro Yuji Hinoda Koji Tamada Shigefumi Yoshino Shoichi Hazama Masaaki Oka 《Cancer immunology, immunotherapy : CII》2015,64(8):1047-1056
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East Asian species of the genera Hybrizon and Ghilaromma are reviewed. Four species of Hybrizon, H. buccatus (Brébisson 1825), H. ghilarovi Tobias, 1988, H. juncoi (Ceballos 1957) and H. flavofacialis Tobias, 1988 and two species of Ghilaromma, G. orientalis Tobias, 1988 and G. ussuriensis Tobias, 1988, were recognized. H. ghilarovi was recorded from Korea, Japan and China, while H. juncoi was recorded from Korea, for the first time. The specimens recorded from Japan as G. fuliginosi (Wilkinson, 1930) by Watanabe (1984) are referred to G. orientalis herein. This species is newly recorded from Korea and Japan. Keys to East Asian species of Hybrizon and the world species of Ghilaromma are also provided. 相似文献
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G Matsuda Y Suzuyama M Setoguchi T Maita B Watanabe T Maekawa T Umegane 《Hoppe-Seyler's Zeitschrift für physiologische Chemie》1978,359(3):357-368
The light chain fraction was separated from rabbit skeletal muscle myosin and four kinds of light chains, L-1, L-2, L-3 and L-4 in the fraction were further isolated by column chromatography using DEAE-cellulose DE-52. After amino-ethylation, the L-2 light chain was digested with trypsin. It was also digested with chymotrypsin and pepsin, respectively, after carboxymethylation. Each of the tryptic, chymotryptic and peptic peptides thus obtained was separated and purified and their amino acid compositions were analyzed. 相似文献
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The reactions of four anti-sex hormone (Estrone, Estradiol, Estriol and Testosterone) antisera were immunohistochemically examined in 109 cancerous and 80 normal and benign thyroid tissues. Four kinds of sex hormones were detected in the tumour cells of 61 cases (56%) of thyroid cancer and in the follicular epithelial cells of 4 cases (5%) of normal and benign thyroid tissues. Among the thyroid cancers, 54 female (61%) and 7 cases in males (33%) were positive for sex hormones. Furthermore, estrogen binding activity was screened histochemically in 36 thyroid tissues of various types, and detected not only in thyroid cancer (6/15 cases), but in normal and benign thyroid tissues (4/21 cases) as well. It was concluded that endogenous estradiol was located in thyroid cancers more frequently in females than in males and that there was estrogen binding activity in the cells of not only thyroid cancers, but also normal and benign thyroid tissues. This is the first report of the demonstration of endogenous sex hormones in thyroid cancer. 相似文献
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Purification and mechanism of activation of a nerve growth factor-sensitive S6 kinase from PC12 cells 总被引:16,自引:0,他引:16
A nerve growth factor (NGF)-sensitive S6 kinase was purified by alkaline lysis of PC12 cells. The activity in lysates from NGF-treated cells was 10-20-fold higher than that from controls. Half-maximal stimulation of the S6 kinase by NGF treatment occurred in approximately 5 min, and the activity returned almost to basal levels by 2 h. A rapid purification method was devised in which crude extract was applied directly to a PBE 94 column after buffer exchange on a PD-10 column (Sephadex G-25 M). The activated S6 kinase was purified at least 673-fold with a recovery of approximately 70%. The S6 kinase has an apparent molecular weight of 45,000 and is highly specific for S6. It is not inhibited by the specific inhibitor of cAMP-dependent protein kinases, or by chlorpromazine or sodium vanadate, nor is it activated by Ca2+/calmodulin. It was inhibited by EGTA, beta-glycerophosphate, or NaF. Phosphorylation occurred solely on serine residues. The S6 kinase activity from control cells and from NGF-treated cells eluted at pH 5.69 and 5.58, respectively, during PBE 94 column chromatography. Pretreatment of crude extract from NGF-stimulated cells with alkaline phosphatase resulted in an elution of the enzyme at the position of S6 kinase from control cells and a concomitant decrease in activity. These results indicate that phosphorylation is involved in the mechanism of S6 kinase activation. 相似文献
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Y Wataya H Satake O Hiraoka T Aji K Morishige J Y Kimura A Ishii A Matsuda T Ueda K Fukukawa 《Nucleic acids symposium series》1986,(17):149-151
Carbocyclic inosine is a potent inhibitor for the growth of the promastigote form of Leishmania tropica and Leishmania donovani. In culture, the EC50 values of carbocyclic inosine are 8.3 X 10(-8) and 1.3 X 10(-7) M for the promastigotes of L. tropica and L. donovani, respectively. On the other hand, it is less toxic towards mouse mammary tumor FM3A cells: the EC50 value is 2.7 X 10(-4)M. Carbocyclic inosine is metabolized by Leishmania promastigotes to give carbocyclic adenosine-5'-triphosphate(aristeromycin-5'-triphosphate) and carbocyclic guanosine-5'-triphosphate. This metabolic conversion provides a mechanism for the parasite-selective toxicity of carbocyclic inosine. Carbocyclic inosine was found to be active against L. donovani amastigotes in an in vivo-like cultivation in vitro. 相似文献