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1.
Yoshinari Maeda Kiyoshi Yoshimura Hiroto Matsui Yoshitaro Shindo Takao Tamesa Yukio Tokumitsu Noriaki Hashimoto Yoshihiro Tokuhisa Kazuhiko Sakamoto Kouhei Sakai Yutaka Suehiro Yuji Hinoda Koji Tamada Shigefumi Yoshino Shoichi Hazama Masaaki Oka 《Cancer immunology, immunotherapy : CII》2015,64(8):1047-1056
2.
K Negishi T Yamashita W Zacharias R D Wells T Bessho H Hayatsu 《Nucleic acids symposium series》1990,(22):109-110
Salt-induced and supercoiling-induced B-Z junctions in pWR756, a plasmid containing (GC)16, were probed with bisulfite-methoxyamine, a modification reagent specific for single-stranded nucleic acids. The modification sites were analyzed with S1 nuclease and the modified cytosines were determined from termination sites of DNA chain elongation by DNA polymerase. The results showed that most accessible cytosines are the same for both types of B-Z junctions. 相似文献
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East Asian species of the genera Hybrizon and Ghilaromma are reviewed. Four species of Hybrizon, H. buccatus (Brébisson 1825), H. ghilarovi Tobias, 1988, H. juncoi (Ceballos 1957) and H. flavofacialis Tobias, 1988 and two species of Ghilaromma, G. orientalis Tobias, 1988 and G. ussuriensis Tobias, 1988, were recognized. H. ghilarovi was recorded from Korea, Japan and China, while H. juncoi was recorded from Korea, for the first time. The specimens recorded from Japan as G. fuliginosi (Wilkinson, 1930) by Watanabe (1984) are referred to G. orientalis herein. This species is newly recorded from Korea and Japan. Keys to East Asian species of Hybrizon and the world species of Ghilaromma are also provided. 相似文献
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Tetsuya Kawai Iwase Kazuhiko Noriko Takaya Yuko Yamaguchi Ryuta Kishii Yasushi Kohno Haruaki Kurasaki 《Bioorganic & medicinal chemistry letters》2017,27(4):1045-1049
We attempted to optimize sulfonamide-based non-alkyne LpxC inhibitors by focusing on improvements in enzyme inhibitory and antibacterial activity. It was discovered that inhibitors possessing 2-aryl benzofuran as a hydrophobe exhibited good activity. In particular, compound 21 displayed impressive antibacterial activity (E. coli MIC = 0.063 μg/mL, K. pneumoniae MIC = 0.5 μg/mL, and P. aeruginosa MIC = 0.5 μg/mL), and is a promising lead for further exploration as an antibacterial agent. 相似文献
7.
H Kasai Z Yamaizumi F Yamamoto T Bessho S Nishimura M Berger J Cadet 《Nucleic acids symposium series》1992,(27):181-182
Potosensitized formation of 8-hydroxyguanine in DNA by riboflavin was observed. A reaction mechanism involving guanine radical cation and hydration reaction was proposed. This hypothesis was confirmed by the incorporation of [18O]-atom within guanine moiety in isotopic experiments using [18O]-H2O. Photosensitized formation of oh8Gua by riboflavin was also observed in cellular DNA. 相似文献
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The AtXTH28 Gene, a Xyloglucan Endotransglucosylase/Hydrolase, is Involved in Automatic Self-Pollination in Arabidopsis thaliana 总被引:1,自引:0,他引:1
Kurasawa Kasumi; Matsui Akihiro; Yokoyama Ryusuke; Kuriyama Tomoko; Yoshizumi Takeshi; Matsui Minami; Suwabe Keita; Watanabe Masao; Nishitani Kazuhiko 《Plant & cell physiology》2009,50(2):413-422
Successful automatic self-pollination in flowering plants isdependent on the correct development of reproductive organs.In the stamen, the appropriate growth of the filament, whichlargely depends on the mechanical properties of the cell wall,is required to position the anther correctly close to the stigmaat the pollination stage. Xyloglucan endotransglucosylase/hydrolases(XTHs) are a family of enzymes that mediate the constructionand restructuring of xyloglucan cross-links, thereby controllingthe extensibility or mechanical properties of the cell wallin a wide variety of plant tissues. Our reverse genetic analysishas revealed that a loss-of-function mutation of an ArabidopsisXTH family gene, AtXTH28, led to a decrease in capability forself-pollination, probably due to inhibition of stamen filamentgrowth. Our results also suggest that the role of AtXTH28 inthe development of the stamen is not functionally redundantwith its closest paralog, AtXTH27. Thus, our finding indicatesthat AtXTH28 is specifically involved in the growth of stamenfilaments, and is required for successful automatic self-pollinationin certain flowers in Arabidopsis thaliana. 相似文献
10.
Regulatory relationship and gain control between cytosolic free Ca2+ concentration (Cai) and cytosolic pH (pHi) were evaluated by two different cell types, gastric parietal cells, and blood platelets. Studies were carried out in both single cells and populations of cells, using Ca2+-indicative probe fura-2 (1-(2-(5′-carboxyoxazol-2′-yl)-6-aminobenzofuran-5-oxy)-2-(2′-amino-5′-methylphenoxy) ethane-N,N,N′,N′-tetraacetic acid) and pH-indicative probe BCECF (2′,7′-bis(carboxyethyl) carboxyfluorescein). Stimulation of single and populational parietal cells and platelets with gastrin and thrombin, respectively, resulted in an increase in Cai. In both populational cell types, an initial change in pHi during agonist stimulation occurred almost simultaneously with the mobilization of Ca2+; an initial transient decrease in pHi was followed by a slower increase in pHi above the prestimulation level. When populational platelets were preloaded with the Ca2+ chelator BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′tetraacetic acid), the thrombin-induced initial large increase in Cai was apparently inhibited, whereas the pHi decrease induced by thrombin was not altered. This suggests that the initial Cai change is not a prerequisite for the pHi change. The effect of pHi on Cai was examined next. In both single and populational cell types, application of the K+-H+ ionophore nigericin, which induced a transient decrease in pHi, led to the release of Ca2+ from intracellular stores. In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin. A decrease in pHi, and an increase in Cai occurred at 1.5 and 4 s, respectively. In single parietal cells, replacement of medium Na+ with N-methyl-
-glucamine (NMG+), which also induced a decrease in pHi, resulted in repetitive Ca2+ spike oscillations. The source of Ca2+ utilized for the Ca2+ oscillation that was induced by NMG+ originated from the agonist-sensitive pool. Thus, several maneuvers, which were capable of decreasing pHi, led to an increase in Cai. Cytosolic acidification may be a part of the trigger for Ca2+ mobilization from intracellular stores in both parietal cells and platelets. 相似文献