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1.
Maximilian Schmid Bianca Dufner Julius Dürk Konstanze Bedal Kristina Stricker Lukas Ali Prokoph Christoph Koch Anja K. Wege Henner Zirpel Ger van Zandbergen Rupert Ecker Bogdan Boghiu Uwe Ritter 《PloS one》2015,10(10)
Characterization of host-pathogen interactions is a fundamental approach in microbiological and immunological oriented disciplines. It is commonly accepted that host cells start to change their phenotype after engulfing pathogens. Techniques such as real time PCR or ELISA were used to characterize the genes encoding proteins that are associated either with pathogen elimination or immune escape mechanisms. Most of such studies were performed in vitro using primary host cells or cell lines. Consequently, the data generated with such approaches reflect the global RNA expression or protein amount recovered from all cells in culture. This is justified when all host cells harbor an equal amount of pathogens under experimental conditions. However, the uptake of pathogens by phagocytic cells is not synchronized. Consequently, there are host cells incorporating different amounts of pathogens that might result in distinct pathogen-induced protein biosynthesis. Therefore, we established a technique able to detect and quantify the number of pathogens in the corresponding host cells using immunofluorescence-based high throughput analysis. Paired with multicolor staining of molecules of interest it is now possible to analyze the infection profile of host cell populations and the corresponding phenotype of the host cells as a result of parasite load. 相似文献
2.
Amanda L. McGuire Sophia C. Bennett Sally M. Lansley Natalia D. Popowicz Julius F. Varano della Vergiliana Daniel Wong Y. C. Gary Lee Aron Chakera 《PloS one》2015,10(3)
A major complication of peritoneal dialysis is the development of peritonitis, which is associated with reduced technique and patient survival. The inflammatory response elicited by infection results in a fibrin and debris-rich environment within the peritoneal cavity, which may reduce the effectiveness of antimicrobial agents and predispose to recurrence or relapse of infection. Strategies to enhance responses to antimicrobial agents therefore have the potential to improve patient outcomes. This study presents pre-clinical data describing the compatibility of tPA and DNase in combination with antimicrobial agents used for the treatment of PD peritonitis. tPA and DNase were stable in standard dialysate solution and in the presence of antimicrobial agents, and were safe when given intraperitoneally in a mouse model with no evidence of local or systemic toxicity. Adjunctive tPA and DNase may have a role in the management of patients presenting with PD peritonitis. 相似文献
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Ohne Zusammenfassung 相似文献
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Julius Głowacki 《Plant Systematics and Evolution》1909,59(2):51-53
Ohne Zusammenfassung 相似文献
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The present investigation assesses a number of explanations for the patterns of variability in dental dimensions. Coefficients of variation were calculated for mesiodistal and buccolingual diameters in a sample of 105 Papio ursinus crania (52 male, 53 female). Variability profiles consisting of arrays of values of coefficients of variation were evaluated by means of Friedman's two-way analysis of variance and Kendall's coefficient of concordance. Although molar teeth were found to be the most dimensionally stable, our results failed to support either the morphogenetic field theory or the occlusal complexity hypothesis. The data presented here are generally supportive of Pengilly's phenotypic complexity theory. However, speciesspecific clustering patterns found in our regressions of dimensional variability on mean tooth size suggest that differences in variability levels might be related to differences in selective pressures. 相似文献
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U Julius W Leonhardt H Schneider K Schollberg M Hanefeld J Schulze H Haller 《Endokrinologie》1979,73(2):214-220
In 17 non-selected, non-hyperlipoproteinemic subjects without overt diabetes both adipose tissue biopsy and an oral glucose tolerance test (50 g) were performed. All persons were weight-stable at the time of investigation. A significant correlation between fasting insulin concentration and mean adipocyte volume was observed, whereas no correlation existed between ideal body weight index and fasting insulin level. Persons with larger adipocytes had elevated basal insulin levels as well as higher and longer lasting increments following the glucose challenge. They also exhibited significantly higher mean glucose levels during the OGTT. When these patients were matched for glucose tolerance with the subgroup having smaller mean adipocyte volumes, the difference in insulin levels was still demonstrable. This study underlines the importance of adipose-cell enlargement regulating basal and stimulated insulin output. 相似文献
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Jacqueline Knobloch Daniel K. Suhendro Julius L. Zieleniecki Joseph G. Shapter Ingo K?per 《Saudi Journal of Biological Sciences》2015,22(6):714-718
The direct interaction of drugs with the cell membrane is often neglected when drug effects are studied. Systematic investigations are hindered by the complexity of the natural membrane and model membrane systems can offer a useful alternative. Here some examples are reviewed of how model membrane architectures including vesicles, Langmuir monolayers and solid supported membranes can be used to investigate the effects of drug molecules on the membrane structure, and how these interactions can translate into effects on embedded membrane proteins. 相似文献